Pan-Enterovirus Amplicon-Based High-Throughput Sequencing Detects the Complete Capsid of a EVA71 Genotype C1 Variant via Wastewater-Based Epidemiology in Arizona

We describe the complete capsid of a genotype C1-like Enterovirus A71 variant recovered from wastewater in a neighborhood in the greater Tempe, Arizona area (Southwest United States) in May 2020 using a pan-enterovirus amplicon-based high-throughput sequencing strategy. The variant seems to have bee...

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Autores principales: Faleye, Temitope O. C., Driver, Erin, Bowes, Devin, Adhikari, Sangeet, Adams, Deborah, Varsani, Arvind, Halden, Rolf U., Scotch, Matthew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827028/
https://www.ncbi.nlm.nih.gov/pubmed/33430521
http://dx.doi.org/10.3390/v13010074
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author Faleye, Temitope O. C.
Driver, Erin
Bowes, Devin
Adhikari, Sangeet
Adams, Deborah
Varsani, Arvind
Halden, Rolf U.
Scotch, Matthew
author_facet Faleye, Temitope O. C.
Driver, Erin
Bowes, Devin
Adhikari, Sangeet
Adams, Deborah
Varsani, Arvind
Halden, Rolf U.
Scotch, Matthew
author_sort Faleye, Temitope O. C.
collection PubMed
description We describe the complete capsid of a genotype C1-like Enterovirus A71 variant recovered from wastewater in a neighborhood in the greater Tempe, Arizona area (Southwest United States) in May 2020 using a pan-enterovirus amplicon-based high-throughput sequencing strategy. The variant seems to have been circulating for over two years, but its sequence has not been documented in that period. As the SARS-CoV-2 pandemic has resulted in changes in health-seeking behavior and overwhelmed pathogen diagnostics, our findings highlight the importance of wastewater-based epidemiology (WBE ) as an early warning system for virus surveillance.
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spelling pubmed-78270282021-01-25 Pan-Enterovirus Amplicon-Based High-Throughput Sequencing Detects the Complete Capsid of a EVA71 Genotype C1 Variant via Wastewater-Based Epidemiology in Arizona Faleye, Temitope O. C. Driver, Erin Bowes, Devin Adhikari, Sangeet Adams, Deborah Varsani, Arvind Halden, Rolf U. Scotch, Matthew Viruses Article We describe the complete capsid of a genotype C1-like Enterovirus A71 variant recovered from wastewater in a neighborhood in the greater Tempe, Arizona area (Southwest United States) in May 2020 using a pan-enterovirus amplicon-based high-throughput sequencing strategy. The variant seems to have been circulating for over two years, but its sequence has not been documented in that period. As the SARS-CoV-2 pandemic has resulted in changes in health-seeking behavior and overwhelmed pathogen diagnostics, our findings highlight the importance of wastewater-based epidemiology (WBE ) as an early warning system for virus surveillance. MDPI 2021-01-07 /pmc/articles/PMC7827028/ /pubmed/33430521 http://dx.doi.org/10.3390/v13010074 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Faleye, Temitope O. C.
Driver, Erin
Bowes, Devin
Adhikari, Sangeet
Adams, Deborah
Varsani, Arvind
Halden, Rolf U.
Scotch, Matthew
Pan-Enterovirus Amplicon-Based High-Throughput Sequencing Detects the Complete Capsid of a EVA71 Genotype C1 Variant via Wastewater-Based Epidemiology in Arizona
title Pan-Enterovirus Amplicon-Based High-Throughput Sequencing Detects the Complete Capsid of a EVA71 Genotype C1 Variant via Wastewater-Based Epidemiology in Arizona
title_full Pan-Enterovirus Amplicon-Based High-Throughput Sequencing Detects the Complete Capsid of a EVA71 Genotype C1 Variant via Wastewater-Based Epidemiology in Arizona
title_fullStr Pan-Enterovirus Amplicon-Based High-Throughput Sequencing Detects the Complete Capsid of a EVA71 Genotype C1 Variant via Wastewater-Based Epidemiology in Arizona
title_full_unstemmed Pan-Enterovirus Amplicon-Based High-Throughput Sequencing Detects the Complete Capsid of a EVA71 Genotype C1 Variant via Wastewater-Based Epidemiology in Arizona
title_short Pan-Enterovirus Amplicon-Based High-Throughput Sequencing Detects the Complete Capsid of a EVA71 Genotype C1 Variant via Wastewater-Based Epidemiology in Arizona
title_sort pan-enterovirus amplicon-based high-throughput sequencing detects the complete capsid of a eva71 genotype c1 variant via wastewater-based epidemiology in arizona
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827028/
https://www.ncbi.nlm.nih.gov/pubmed/33430521
http://dx.doi.org/10.3390/v13010074
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