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Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates

Candida parapsilosis is a frequent cause of fungal bloodstream infections, especially in critically ill neonates or immunocompromised patients. Due to the formation of biofilms, the use of indwelling catheters and other medical devices increases the risk of infection and complicates treatment, as ce...

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Autores principales: Gómez-Molero, Emilia, De-la-Pinta, Iker, Fernández-Pereira, Jordan, Groß, Uwe, Weig, Michael, Quindós, Guillermo, de Groot, Piet W. J., Bader, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827155/
https://www.ncbi.nlm.nih.gov/pubmed/33430377
http://dx.doi.org/10.3390/jof7010033
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author Gómez-Molero, Emilia
De-la-Pinta, Iker
Fernández-Pereira, Jordan
Groß, Uwe
Weig, Michael
Quindós, Guillermo
de Groot, Piet W. J.
Bader, Oliver
author_facet Gómez-Molero, Emilia
De-la-Pinta, Iker
Fernández-Pereira, Jordan
Groß, Uwe
Weig, Michael
Quindós, Guillermo
de Groot, Piet W. J.
Bader, Oliver
author_sort Gómez-Molero, Emilia
collection PubMed
description Candida parapsilosis is a frequent cause of fungal bloodstream infections, especially in critically ill neonates or immunocompromised patients. Due to the formation of biofilms, the use of indwelling catheters and other medical devices increases the risk of infection and complicates treatment, as cells embedded in biofilms display reduced drug susceptibility. Therefore, biofilm formation may be a significant clinical parameter, guiding downstream therapeutic choices. Here, we phenotypically characterized 120 selected isolates out of a prospective collection of 215 clinical C. parapsilosis isolates, determining biofilm formation, major emerging colony morphotype, and antifungal drug susceptibility of the isolates and their biofilms. In our isolate set, increased biofilm formation capacity was independent of body site of isolation and not predictable using standard or modified European Committee on Antimicrobial Susceptibility Testing (EUCAST) drug susceptibility testing protocols. In contrast, biofilm formation was strongly correlated with the appearance of non-smooth colony morphotypes and invasiveness into agar plates. Our data suggest that the observation of non-smooth colony morphotypes in cultures of C. parapsilosis may help as an indicator to consider the initiation of anti-biofilm-active therapy, such as the switch from azole- to echinocandin- or polyene-based strategies, especially in case of infections by potent biofilm-forming strains.
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spelling pubmed-78271552021-01-25 Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates Gómez-Molero, Emilia De-la-Pinta, Iker Fernández-Pereira, Jordan Groß, Uwe Weig, Michael Quindós, Guillermo de Groot, Piet W. J. Bader, Oliver J Fungi (Basel) Article Candida parapsilosis is a frequent cause of fungal bloodstream infections, especially in critically ill neonates or immunocompromised patients. Due to the formation of biofilms, the use of indwelling catheters and other medical devices increases the risk of infection and complicates treatment, as cells embedded in biofilms display reduced drug susceptibility. Therefore, biofilm formation may be a significant clinical parameter, guiding downstream therapeutic choices. Here, we phenotypically characterized 120 selected isolates out of a prospective collection of 215 clinical C. parapsilosis isolates, determining biofilm formation, major emerging colony morphotype, and antifungal drug susceptibility of the isolates and their biofilms. In our isolate set, increased biofilm formation capacity was independent of body site of isolation and not predictable using standard or modified European Committee on Antimicrobial Susceptibility Testing (EUCAST) drug susceptibility testing protocols. In contrast, biofilm formation was strongly correlated with the appearance of non-smooth colony morphotypes and invasiveness into agar plates. Our data suggest that the observation of non-smooth colony morphotypes in cultures of C. parapsilosis may help as an indicator to consider the initiation of anti-biofilm-active therapy, such as the switch from azole- to echinocandin- or polyene-based strategies, especially in case of infections by potent biofilm-forming strains. MDPI 2021-01-07 /pmc/articles/PMC7827155/ /pubmed/33430377 http://dx.doi.org/10.3390/jof7010033 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gómez-Molero, Emilia
De-la-Pinta, Iker
Fernández-Pereira, Jordan
Groß, Uwe
Weig, Michael
Quindós, Guillermo
de Groot, Piet W. J.
Bader, Oliver
Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates
title Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates
title_full Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates
title_fullStr Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates
title_full_unstemmed Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates
title_short Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates
title_sort candida parapsilosis colony morphotype forecasts biofilm formation of clinical isolates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827155/
https://www.ncbi.nlm.nih.gov/pubmed/33430377
http://dx.doi.org/10.3390/jof7010033
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