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Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition

Sleeping Beauty (SB) is a transposon system that has been widely used as a genetic engineering tool. Central to the development of any transposon as a research tool is the ability to integrate a foreign piece of DNA into the cellular genome. Driven by the need for efficient transposon-based gene vec...

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Detalles Bibliográficos
Autores principales: Ochmann, Matthias T., Ivics, Zoltán
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827188/
https://www.ncbi.nlm.nih.gov/pubmed/33429848
http://dx.doi.org/10.3390/v13010076
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author Ochmann, Matthias T.
Ivics, Zoltán
author_facet Ochmann, Matthias T.
Ivics, Zoltán
author_sort Ochmann, Matthias T.
collection PubMed
description Sleeping Beauty (SB) is a transposon system that has been widely used as a genetic engineering tool. Central to the development of any transposon as a research tool is the ability to integrate a foreign piece of DNA into the cellular genome. Driven by the need for efficient transposon-based gene vector systems, extensive studies have largely elucidated the molecular actors and actions taking place during SB transposition. Close transposon relatives and other recombination enzymes, including retroviral integrases, have served as useful models to infer functional information relevant to SB. Recently obtained structural data on the SB transposase enable a direct insight into the workings of this enzyme. These efforts cumulatively allowed the development of novel variants of SB that offer advanced possibilities for genetic engineering due to their hyperactivity, integration deficiency, or targeting capacity. However, many aspects of the process of transposition remain poorly understood and require further investigation. We anticipate that continued investigations into the structure–function relationships of SB transposition will enable the development of new generations of transposition-based vector systems, thereby facilitating the use of SB in preclinical studies and clinical trials.
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spelling pubmed-78271882021-01-25 Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition Ochmann, Matthias T. Ivics, Zoltán Viruses Review Sleeping Beauty (SB) is a transposon system that has been widely used as a genetic engineering tool. Central to the development of any transposon as a research tool is the ability to integrate a foreign piece of DNA into the cellular genome. Driven by the need for efficient transposon-based gene vector systems, extensive studies have largely elucidated the molecular actors and actions taking place during SB transposition. Close transposon relatives and other recombination enzymes, including retroviral integrases, have served as useful models to infer functional information relevant to SB. Recently obtained structural data on the SB transposase enable a direct insight into the workings of this enzyme. These efforts cumulatively allowed the development of novel variants of SB that offer advanced possibilities for genetic engineering due to their hyperactivity, integration deficiency, or targeting capacity. However, many aspects of the process of transposition remain poorly understood and require further investigation. We anticipate that continued investigations into the structure–function relationships of SB transposition will enable the development of new generations of transposition-based vector systems, thereby facilitating the use of SB in preclinical studies and clinical trials. MDPI 2021-01-08 /pmc/articles/PMC7827188/ /pubmed/33429848 http://dx.doi.org/10.3390/v13010076 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Ochmann, Matthias T.
Ivics, Zoltán
Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition
title Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition
title_full Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition
title_fullStr Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition
title_full_unstemmed Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition
title_short Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition
title_sort jumping ahead with sleeping beauty: mechanistic insights into cut-and-paste transposition
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827188/
https://www.ncbi.nlm.nih.gov/pubmed/33429848
http://dx.doi.org/10.3390/v13010076
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