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Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition
Sleeping Beauty (SB) is a transposon system that has been widely used as a genetic engineering tool. Central to the development of any transposon as a research tool is the ability to integrate a foreign piece of DNA into the cellular genome. Driven by the need for efficient transposon-based gene vec...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827188/ https://www.ncbi.nlm.nih.gov/pubmed/33429848 http://dx.doi.org/10.3390/v13010076 |
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author | Ochmann, Matthias T. Ivics, Zoltán |
author_facet | Ochmann, Matthias T. Ivics, Zoltán |
author_sort | Ochmann, Matthias T. |
collection | PubMed |
description | Sleeping Beauty (SB) is a transposon system that has been widely used as a genetic engineering tool. Central to the development of any transposon as a research tool is the ability to integrate a foreign piece of DNA into the cellular genome. Driven by the need for efficient transposon-based gene vector systems, extensive studies have largely elucidated the molecular actors and actions taking place during SB transposition. Close transposon relatives and other recombination enzymes, including retroviral integrases, have served as useful models to infer functional information relevant to SB. Recently obtained structural data on the SB transposase enable a direct insight into the workings of this enzyme. These efforts cumulatively allowed the development of novel variants of SB that offer advanced possibilities for genetic engineering due to their hyperactivity, integration deficiency, or targeting capacity. However, many aspects of the process of transposition remain poorly understood and require further investigation. We anticipate that continued investigations into the structure–function relationships of SB transposition will enable the development of new generations of transposition-based vector systems, thereby facilitating the use of SB in preclinical studies and clinical trials. |
format | Online Article Text |
id | pubmed-7827188 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78271882021-01-25 Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition Ochmann, Matthias T. Ivics, Zoltán Viruses Review Sleeping Beauty (SB) is a transposon system that has been widely used as a genetic engineering tool. Central to the development of any transposon as a research tool is the ability to integrate a foreign piece of DNA into the cellular genome. Driven by the need for efficient transposon-based gene vector systems, extensive studies have largely elucidated the molecular actors and actions taking place during SB transposition. Close transposon relatives and other recombination enzymes, including retroviral integrases, have served as useful models to infer functional information relevant to SB. Recently obtained structural data on the SB transposase enable a direct insight into the workings of this enzyme. These efforts cumulatively allowed the development of novel variants of SB that offer advanced possibilities for genetic engineering due to their hyperactivity, integration deficiency, or targeting capacity. However, many aspects of the process of transposition remain poorly understood and require further investigation. We anticipate that continued investigations into the structure–function relationships of SB transposition will enable the development of new generations of transposition-based vector systems, thereby facilitating the use of SB in preclinical studies and clinical trials. MDPI 2021-01-08 /pmc/articles/PMC7827188/ /pubmed/33429848 http://dx.doi.org/10.3390/v13010076 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Ochmann, Matthias T. Ivics, Zoltán Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition |
title | Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition |
title_full | Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition |
title_fullStr | Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition |
title_full_unstemmed | Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition |
title_short | Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition |
title_sort | jumping ahead with sleeping beauty: mechanistic insights into cut-and-paste transposition |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827188/ https://www.ncbi.nlm.nih.gov/pubmed/33429848 http://dx.doi.org/10.3390/v13010076 |
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