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The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia (Oreochromis niloticus) and Atlantic Cod (Gadus morhua L.)
Francisellosis in fish is caused by the facultative intracellular Gram-negative bacterial pathogens Francisella noatunensis ssp. noatunensis and Francisella orientalis. The disease is affecting both farmed and wild fish worldwide and no commercial vaccines are currently available. In this study, we...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827370/ https://www.ncbi.nlm.nih.gov/pubmed/33435503 http://dx.doi.org/10.3390/vaccines9010034 |
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author | Mertes, Verena Bekkelund, Alexander Kashulin Lagos, Leidy Ciani, Elia Colquhoun, Duncan Haslene-Hox, Hanne Sletta, Håvard Sørum, Henning Winther-Larsen, Hanne Cecilie |
author_facet | Mertes, Verena Bekkelund, Alexander Kashulin Lagos, Leidy Ciani, Elia Colquhoun, Duncan Haslene-Hox, Hanne Sletta, Håvard Sørum, Henning Winther-Larsen, Hanne Cecilie |
author_sort | Mertes, Verena |
collection | PubMed |
description | Francisellosis in fish is caused by the facultative intracellular Gram-negative bacterial pathogens Francisella noatunensis ssp. noatunensis and Francisella orientalis. The disease is affecting both farmed and wild fish worldwide and no commercial vaccines are currently available. In this study, we tested isolated membrane vesicles (MVs) as possible vaccine candidates based on previous trials in zebrafish (Danio rerio) indicating promising vaccine efficacy. Here, the MV vaccine-candidates were tested in their natural hosts, Atlantic cod (Gadus morhua L.) and Nile tilapia (Oreochromis niloticus). Injection of MVs did not display any toxicity or other negative influence on the fish and gene expression analysis indicated an influence on the host immune response. However, unlike in other tested fish species, a protective immunity following vaccine application and immunization period could not be detected in the Atlantic cod or tilapia. Further in vivo studies are required to achieve a better understanding of the development of immunological memory in different fish species. |
format | Online Article Text |
id | pubmed-7827370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78273702021-01-25 The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia (Oreochromis niloticus) and Atlantic Cod (Gadus morhua L.) Mertes, Verena Bekkelund, Alexander Kashulin Lagos, Leidy Ciani, Elia Colquhoun, Duncan Haslene-Hox, Hanne Sletta, Håvard Sørum, Henning Winther-Larsen, Hanne Cecilie Vaccines (Basel) Article Francisellosis in fish is caused by the facultative intracellular Gram-negative bacterial pathogens Francisella noatunensis ssp. noatunensis and Francisella orientalis. The disease is affecting both farmed and wild fish worldwide and no commercial vaccines are currently available. In this study, we tested isolated membrane vesicles (MVs) as possible vaccine candidates based on previous trials in zebrafish (Danio rerio) indicating promising vaccine efficacy. Here, the MV vaccine-candidates were tested in their natural hosts, Atlantic cod (Gadus morhua L.) and Nile tilapia (Oreochromis niloticus). Injection of MVs did not display any toxicity or other negative influence on the fish and gene expression analysis indicated an influence on the host immune response. However, unlike in other tested fish species, a protective immunity following vaccine application and immunization period could not be detected in the Atlantic cod or tilapia. Further in vivo studies are required to achieve a better understanding of the development of immunological memory in different fish species. MDPI 2021-01-09 /pmc/articles/PMC7827370/ /pubmed/33435503 http://dx.doi.org/10.3390/vaccines9010034 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mertes, Verena Bekkelund, Alexander Kashulin Lagos, Leidy Ciani, Elia Colquhoun, Duncan Haslene-Hox, Hanne Sletta, Håvard Sørum, Henning Winther-Larsen, Hanne Cecilie The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia (Oreochromis niloticus) and Atlantic Cod (Gadus morhua L.) |
title | The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia (Oreochromis niloticus) and Atlantic Cod (Gadus morhua L.) |
title_full | The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia (Oreochromis niloticus) and Atlantic Cod (Gadus morhua L.) |
title_fullStr | The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia (Oreochromis niloticus) and Atlantic Cod (Gadus morhua L.) |
title_full_unstemmed | The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia (Oreochromis niloticus) and Atlantic Cod (Gadus morhua L.) |
title_short | The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia (Oreochromis niloticus) and Atlantic Cod (Gadus morhua L.) |
title_sort | use of extracellular membrane vesicles for immunization against francisellosis in nile tilapia (oreochromis niloticus) and atlantic cod (gadus morhua l.) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827370/ https://www.ncbi.nlm.nih.gov/pubmed/33435503 http://dx.doi.org/10.3390/vaccines9010034 |
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