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Electrically Conductive Micropatterned Polyaniline-Poly(ethylene glycol) Composite Hydrogel

Hydrogel substrate-based micropatterns can be adjusted using the pattern shape and size, affecting cell behaviors such as proliferation and differentiation under various cellular environment parameters. An electrically conductive hydrogel pattern system mimics the native muscle tissue environment. I...

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Detalles Bibliográficos
Autores principales: Noh, Soyoung, Gong, Hye Yeon, Lee, Hyun Jong, Koh, Won-Gun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827658/
https://www.ncbi.nlm.nih.gov/pubmed/33435614
http://dx.doi.org/10.3390/ma14020308
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author Noh, Soyoung
Gong, Hye Yeon
Lee, Hyun Jong
Koh, Won-Gun
author_facet Noh, Soyoung
Gong, Hye Yeon
Lee, Hyun Jong
Koh, Won-Gun
author_sort Noh, Soyoung
collection PubMed
description Hydrogel substrate-based micropatterns can be adjusted using the pattern shape and size, affecting cell behaviors such as proliferation and differentiation under various cellular environment parameters. An electrically conductive hydrogel pattern system mimics the native muscle tissue environment. In this study, we incorporated polyaniline (PANi) in a poly(ethylene glycol) (PEG) hydrogel matrix through UV-induced photolithography with photomasks, and electrically conductive hydrogel micropatterns were generated within a few seconds. The electrical conductance of the PANi/PEG hydrogel was 30.5 ± 0.5 mS/cm. C2C12 myoblasts were cultured on the resulting substrate, and the cells adhered selectively to the PANi/PEG hydrogel regions. Myogenic differentiation of the C2C12 cells was induced, and the alignment of myotubes was consistent with the arrangement of the line pattern. The expression of myosin heavy chain on the line pattern showed potential as a substrate for myogenic cell functionalization.
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spelling pubmed-78276582021-01-25 Electrically Conductive Micropatterned Polyaniline-Poly(ethylene glycol) Composite Hydrogel Noh, Soyoung Gong, Hye Yeon Lee, Hyun Jong Koh, Won-Gun Materials (Basel) Article Hydrogel substrate-based micropatterns can be adjusted using the pattern shape and size, affecting cell behaviors such as proliferation and differentiation under various cellular environment parameters. An electrically conductive hydrogel pattern system mimics the native muscle tissue environment. In this study, we incorporated polyaniline (PANi) in a poly(ethylene glycol) (PEG) hydrogel matrix through UV-induced photolithography with photomasks, and electrically conductive hydrogel micropatterns were generated within a few seconds. The electrical conductance of the PANi/PEG hydrogel was 30.5 ± 0.5 mS/cm. C2C12 myoblasts were cultured on the resulting substrate, and the cells adhered selectively to the PANi/PEG hydrogel regions. Myogenic differentiation of the C2C12 cells was induced, and the alignment of myotubes was consistent with the arrangement of the line pattern. The expression of myosin heavy chain on the line pattern showed potential as a substrate for myogenic cell functionalization. MDPI 2021-01-08 /pmc/articles/PMC7827658/ /pubmed/33435614 http://dx.doi.org/10.3390/ma14020308 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Noh, Soyoung
Gong, Hye Yeon
Lee, Hyun Jong
Koh, Won-Gun
Electrically Conductive Micropatterned Polyaniline-Poly(ethylene glycol) Composite Hydrogel
title Electrically Conductive Micropatterned Polyaniline-Poly(ethylene glycol) Composite Hydrogel
title_full Electrically Conductive Micropatterned Polyaniline-Poly(ethylene glycol) Composite Hydrogel
title_fullStr Electrically Conductive Micropatterned Polyaniline-Poly(ethylene glycol) Composite Hydrogel
title_full_unstemmed Electrically Conductive Micropatterned Polyaniline-Poly(ethylene glycol) Composite Hydrogel
title_short Electrically Conductive Micropatterned Polyaniline-Poly(ethylene glycol) Composite Hydrogel
title_sort electrically conductive micropatterned polyaniline-poly(ethylene glycol) composite hydrogel
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827658/
https://www.ncbi.nlm.nih.gov/pubmed/33435614
http://dx.doi.org/10.3390/ma14020308
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