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Concurrent Prebiotic Intake Reverses Insulin Resistance Induced by Early-Life Pulsed Antibiotic in Rats

Pulsed antibiotic treatment (PAT) early in life increases risk of obesity. Prebiotics can reduce fat mass and improve metabolic health. We examined if co-administering prebiotic with PAT reduces obesity risk in rat pups weaned onto a high fat/sucrose diet. Pups were randomized to (1) control [CTR],...

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Autores principales: Klancic, Teja, Laforest-Lapointe, Isabelle, Wong, Jolene, Choo, Ashley, Nettleton, Jodi E., Chleilat, Faye, Arrieta, Marie-Claire, Reimer, Raylene A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827688/
https://www.ncbi.nlm.nih.gov/pubmed/33445530
http://dx.doi.org/10.3390/biomedicines9010066
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author Klancic, Teja
Laforest-Lapointe, Isabelle
Wong, Jolene
Choo, Ashley
Nettleton, Jodi E.
Chleilat, Faye
Arrieta, Marie-Claire
Reimer, Raylene A.
author_facet Klancic, Teja
Laforest-Lapointe, Isabelle
Wong, Jolene
Choo, Ashley
Nettleton, Jodi E.
Chleilat, Faye
Arrieta, Marie-Claire
Reimer, Raylene A.
author_sort Klancic, Teja
collection PubMed
description Pulsed antibiotic treatment (PAT) early in life increases risk of obesity. Prebiotics can reduce fat mass and improve metabolic health. We examined if co-administering prebiotic with PAT reduces obesity risk in rat pups weaned onto a high fat/sucrose diet. Pups were randomized to (1) control [CTR], (2) antibiotic [ABT] (azithromycin), (3) prebiotic [PRE] (10% oligofructose (OFS)), (4) antibiotic + prebiotic [ABT + PRE]. Pulses of antibiotics/prebiotics were administered at d19–21, d28–30 and d37–39. Male and female rats given antibiotics (ABT) had higher body weight than all other groups at 10 wk of age. The PAT phenotype was stronger in ABT males than females, where increased fat mass, hyperinsulinemia and insulin resistance were present and all reversible with prebiotics. Reduced hypothalamic and hepatic expression of insulin receptor substrates and ileal tight junction proteins was seen in males only, explaining their greater insulin resistance. In females, insulin resistance was improved with prebiotics and normalized to lean control. ABT reduced Lactobacillaceae and increased Bacteroidaceae in both sexes. Using a therapeutic dose of an antibiotic commonly used for acute infection in children, PAT increased body weight and impaired insulin production and insulin sensitivity. The effects were reversed with prebiotic co-administration in a sex-specific manner.
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spelling pubmed-78276882021-01-25 Concurrent Prebiotic Intake Reverses Insulin Resistance Induced by Early-Life Pulsed Antibiotic in Rats Klancic, Teja Laforest-Lapointe, Isabelle Wong, Jolene Choo, Ashley Nettleton, Jodi E. Chleilat, Faye Arrieta, Marie-Claire Reimer, Raylene A. Biomedicines Article Pulsed antibiotic treatment (PAT) early in life increases risk of obesity. Prebiotics can reduce fat mass and improve metabolic health. We examined if co-administering prebiotic with PAT reduces obesity risk in rat pups weaned onto a high fat/sucrose diet. Pups were randomized to (1) control [CTR], (2) antibiotic [ABT] (azithromycin), (3) prebiotic [PRE] (10% oligofructose (OFS)), (4) antibiotic + prebiotic [ABT + PRE]. Pulses of antibiotics/prebiotics were administered at d19–21, d28–30 and d37–39. Male and female rats given antibiotics (ABT) had higher body weight than all other groups at 10 wk of age. The PAT phenotype was stronger in ABT males than females, where increased fat mass, hyperinsulinemia and insulin resistance were present and all reversible with prebiotics. Reduced hypothalamic and hepatic expression of insulin receptor substrates and ileal tight junction proteins was seen in males only, explaining their greater insulin resistance. In females, insulin resistance was improved with prebiotics and normalized to lean control. ABT reduced Lactobacillaceae and increased Bacteroidaceae in both sexes. Using a therapeutic dose of an antibiotic commonly used for acute infection in children, PAT increased body weight and impaired insulin production and insulin sensitivity. The effects were reversed with prebiotic co-administration in a sex-specific manner. MDPI 2021-01-12 /pmc/articles/PMC7827688/ /pubmed/33445530 http://dx.doi.org/10.3390/biomedicines9010066 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Klancic, Teja
Laforest-Lapointe, Isabelle
Wong, Jolene
Choo, Ashley
Nettleton, Jodi E.
Chleilat, Faye
Arrieta, Marie-Claire
Reimer, Raylene A.
Concurrent Prebiotic Intake Reverses Insulin Resistance Induced by Early-Life Pulsed Antibiotic in Rats
title Concurrent Prebiotic Intake Reverses Insulin Resistance Induced by Early-Life Pulsed Antibiotic in Rats
title_full Concurrent Prebiotic Intake Reverses Insulin Resistance Induced by Early-Life Pulsed Antibiotic in Rats
title_fullStr Concurrent Prebiotic Intake Reverses Insulin Resistance Induced by Early-Life Pulsed Antibiotic in Rats
title_full_unstemmed Concurrent Prebiotic Intake Reverses Insulin Resistance Induced by Early-Life Pulsed Antibiotic in Rats
title_short Concurrent Prebiotic Intake Reverses Insulin Resistance Induced by Early-Life Pulsed Antibiotic in Rats
title_sort concurrent prebiotic intake reverses insulin resistance induced by early-life pulsed antibiotic in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827688/
https://www.ncbi.nlm.nih.gov/pubmed/33445530
http://dx.doi.org/10.3390/biomedicines9010066
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