Cargando…

Comparison of Light Condition-Dependent Differences in the Accumulation and Subcellular Localization of Glutathione in Arabidopsis and Wheat

This study aimed to clarify whether the light condition-dependent changes in the redox state and subcellular distribution of glutathione were similar in the dicotyledonous model plant Arabidopsis (wild-type, ascorbate- and glutathione-deficient mutants) and the monocotyledonous crop species wheat (C...

Descripción completa

Detalles Bibliográficos
Autores principales: Gasperl, Anna, Balogh, Eszter, Boldizsár, Ákos, Kemeter, Nadine, Pirklbauer, Richard, Möstl, Stefan, Kalapos, Balázs, Szalai, Gabriella, Müller, Maria, Zellnig, Günther, Kocsy, Gábor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827723/
https://www.ncbi.nlm.nih.gov/pubmed/33435361
http://dx.doi.org/10.3390/ijms22020607
_version_ 1783640836488036352
author Gasperl, Anna
Balogh, Eszter
Boldizsár, Ákos
Kemeter, Nadine
Pirklbauer, Richard
Möstl, Stefan
Kalapos, Balázs
Szalai, Gabriella
Müller, Maria
Zellnig, Günther
Kocsy, Gábor
author_facet Gasperl, Anna
Balogh, Eszter
Boldizsár, Ákos
Kemeter, Nadine
Pirklbauer, Richard
Möstl, Stefan
Kalapos, Balázs
Szalai, Gabriella
Müller, Maria
Zellnig, Günther
Kocsy, Gábor
author_sort Gasperl, Anna
collection PubMed
description This study aimed to clarify whether the light condition-dependent changes in the redox state and subcellular distribution of glutathione were similar in the dicotyledonous model plant Arabidopsis (wild-type, ascorbate- and glutathione-deficient mutants) and the monocotyledonous crop species wheat (Chinese Spring variety). With increasing light intensity, the amount of its reduced (GSH) and oxidized (GSSG) form and the GSSG/GSH ratio increased in the leaf extracts of both species including all genotypes, while far-red light increased these parameters only in wheat except for GSH in the GSH-deficient Arabidopsis mutant. Based on the expression changes of the glutathione metabolism-related genes, light intensity influences the size and redox state of the glutathione pool at the transcriptional level in wheat but not in Arabidopsis. In line with the results in leaf extracts, a similar inducing effect of both light intensity and far-red light was found on the total glutathione content at the subcellular level in wheat. In contrast to the leaf extracts, the inducing influence of light intensity on glutathione level was only found in the cell compartments of the GSH-deficient Arabidopsis mutant, and far-red light increased it in both mutants. The observed general and genotype-specific, light-dependent changes in the accumulation and subcellular distribution of glutathione participate in adjusting the redox-dependent metabolism to the actual environmental conditions.
format Online
Article
Text
id pubmed-7827723
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-78277232021-01-25 Comparison of Light Condition-Dependent Differences in the Accumulation and Subcellular Localization of Glutathione in Arabidopsis and Wheat Gasperl, Anna Balogh, Eszter Boldizsár, Ákos Kemeter, Nadine Pirklbauer, Richard Möstl, Stefan Kalapos, Balázs Szalai, Gabriella Müller, Maria Zellnig, Günther Kocsy, Gábor Int J Mol Sci Article This study aimed to clarify whether the light condition-dependent changes in the redox state and subcellular distribution of glutathione were similar in the dicotyledonous model plant Arabidopsis (wild-type, ascorbate- and glutathione-deficient mutants) and the monocotyledonous crop species wheat (Chinese Spring variety). With increasing light intensity, the amount of its reduced (GSH) and oxidized (GSSG) form and the GSSG/GSH ratio increased in the leaf extracts of both species including all genotypes, while far-red light increased these parameters only in wheat except for GSH in the GSH-deficient Arabidopsis mutant. Based on the expression changes of the glutathione metabolism-related genes, light intensity influences the size and redox state of the glutathione pool at the transcriptional level in wheat but not in Arabidopsis. In line with the results in leaf extracts, a similar inducing effect of both light intensity and far-red light was found on the total glutathione content at the subcellular level in wheat. In contrast to the leaf extracts, the inducing influence of light intensity on glutathione level was only found in the cell compartments of the GSH-deficient Arabidopsis mutant, and far-red light increased it in both mutants. The observed general and genotype-specific, light-dependent changes in the accumulation and subcellular distribution of glutathione participate in adjusting the redox-dependent metabolism to the actual environmental conditions. MDPI 2021-01-09 /pmc/articles/PMC7827723/ /pubmed/33435361 http://dx.doi.org/10.3390/ijms22020607 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gasperl, Anna
Balogh, Eszter
Boldizsár, Ákos
Kemeter, Nadine
Pirklbauer, Richard
Möstl, Stefan
Kalapos, Balázs
Szalai, Gabriella
Müller, Maria
Zellnig, Günther
Kocsy, Gábor
Comparison of Light Condition-Dependent Differences in the Accumulation and Subcellular Localization of Glutathione in Arabidopsis and Wheat
title Comparison of Light Condition-Dependent Differences in the Accumulation and Subcellular Localization of Glutathione in Arabidopsis and Wheat
title_full Comparison of Light Condition-Dependent Differences in the Accumulation and Subcellular Localization of Glutathione in Arabidopsis and Wheat
title_fullStr Comparison of Light Condition-Dependent Differences in the Accumulation and Subcellular Localization of Glutathione in Arabidopsis and Wheat
title_full_unstemmed Comparison of Light Condition-Dependent Differences in the Accumulation and Subcellular Localization of Glutathione in Arabidopsis and Wheat
title_short Comparison of Light Condition-Dependent Differences in the Accumulation and Subcellular Localization of Glutathione in Arabidopsis and Wheat
title_sort comparison of light condition-dependent differences in the accumulation and subcellular localization of glutathione in arabidopsis and wheat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827723/
https://www.ncbi.nlm.nih.gov/pubmed/33435361
http://dx.doi.org/10.3390/ijms22020607
work_keys_str_mv AT gasperlanna comparisonoflightconditiondependentdifferencesintheaccumulationandsubcellularlocalizationofglutathioneinarabidopsisandwheat
AT balogheszter comparisonoflightconditiondependentdifferencesintheaccumulationandsubcellularlocalizationofglutathioneinarabidopsisandwheat
AT boldizsarakos comparisonoflightconditiondependentdifferencesintheaccumulationandsubcellularlocalizationofglutathioneinarabidopsisandwheat
AT kemeternadine comparisonoflightconditiondependentdifferencesintheaccumulationandsubcellularlocalizationofglutathioneinarabidopsisandwheat
AT pirklbauerrichard comparisonoflightconditiondependentdifferencesintheaccumulationandsubcellularlocalizationofglutathioneinarabidopsisandwheat
AT mostlstefan comparisonoflightconditiondependentdifferencesintheaccumulationandsubcellularlocalizationofglutathioneinarabidopsisandwheat
AT kalaposbalazs comparisonoflightconditiondependentdifferencesintheaccumulationandsubcellularlocalizationofglutathioneinarabidopsisandwheat
AT szalaigabriella comparisonoflightconditiondependentdifferencesintheaccumulationandsubcellularlocalizationofglutathioneinarabidopsisandwheat
AT mullermaria comparisonoflightconditiondependentdifferencesintheaccumulationandsubcellularlocalizationofglutathioneinarabidopsisandwheat
AT zellniggunther comparisonoflightconditiondependentdifferencesintheaccumulationandsubcellularlocalizationofglutathioneinarabidopsisandwheat
AT kocsygabor comparisonoflightconditiondependentdifferencesintheaccumulationandsubcellularlocalizationofglutathioneinarabidopsisandwheat