Cargando…

Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity

Dietary salt uptake and inflammation promote sodium accumulation in tissues, thereby modulating cells like macrophages and fibroblasts. Previous studies showed salt effects on periodontal ligament fibroblasts and on bone metabolism by expression of nuclear factor of activated T-cells-5 (NFAT-5). Her...

Descripción completa

Detalles Bibliográficos
Autores principales: Schröder, Agnes, Gubernator, Joshua, Leikam, Alexandra, Nazet, Ute, Cieplik, Fabian, Jantsch, Jonathan, Neubert, Patrick, Titze, Jens, Proff, Peter, Kirschneck, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827744/
https://www.ncbi.nlm.nih.gov/pubmed/33435280
http://dx.doi.org/10.3390/ijms22020596
_version_ 1783640841626058752
author Schröder, Agnes
Gubernator, Joshua
Leikam, Alexandra
Nazet, Ute
Cieplik, Fabian
Jantsch, Jonathan
Neubert, Patrick
Titze, Jens
Proff, Peter
Kirschneck, Christian
author_facet Schröder, Agnes
Gubernator, Joshua
Leikam, Alexandra
Nazet, Ute
Cieplik, Fabian
Jantsch, Jonathan
Neubert, Patrick
Titze, Jens
Proff, Peter
Kirschneck, Christian
author_sort Schröder, Agnes
collection PubMed
description Dietary salt uptake and inflammation promote sodium accumulation in tissues, thereby modulating cells like macrophages and fibroblasts. Previous studies showed salt effects on periodontal ligament fibroblasts and on bone metabolism by expression of nuclear factor of activated T-cells-5 (NFAT-5). Here, we investigated the impact of salt and NFAT-5 on osteoclast activity and orthodontic tooth movement (OTM). After treatment of osteoclasts without (NS) or with additional salt (HS), we analyzed gene expression and the release of tartrate-resistant acid phosphatase and calcium phosphate resorption. We kept wild-type mice and mice lacking NFAT-5 in myeloid cells either on a low, normal or high salt diet and inserted an elastic band between the first and second molar to induce OTM. We analyzed the expression of genes involved in bone metabolism, periodontal bone loss, OTM and bone density. Osteoclast activity was increased upon HS treatment. HS promoted periodontal bone loss and OTM and was associated with reduced bone density. Deletion of NFAT-5 led to increased osteoclast activity with NS, whereas we detected impaired OTM in mice. Dietary salt uptake seems to accelerate OTM and induce periodontal bone loss due to reduced bone density, which may be attributed to enhanced osteoclast activity. NFAT-5 influences this reaction to HS, as we detected impaired OTM and osteoclast activity upon deletion.
format Online
Article
Text
id pubmed-7827744
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-78277442021-01-25 Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity Schröder, Agnes Gubernator, Joshua Leikam, Alexandra Nazet, Ute Cieplik, Fabian Jantsch, Jonathan Neubert, Patrick Titze, Jens Proff, Peter Kirschneck, Christian Int J Mol Sci Article Dietary salt uptake and inflammation promote sodium accumulation in tissues, thereby modulating cells like macrophages and fibroblasts. Previous studies showed salt effects on periodontal ligament fibroblasts and on bone metabolism by expression of nuclear factor of activated T-cells-5 (NFAT-5). Here, we investigated the impact of salt and NFAT-5 on osteoclast activity and orthodontic tooth movement (OTM). After treatment of osteoclasts without (NS) or with additional salt (HS), we analyzed gene expression and the release of tartrate-resistant acid phosphatase and calcium phosphate resorption. We kept wild-type mice and mice lacking NFAT-5 in myeloid cells either on a low, normal or high salt diet and inserted an elastic band between the first and second molar to induce OTM. We analyzed the expression of genes involved in bone metabolism, periodontal bone loss, OTM and bone density. Osteoclast activity was increased upon HS treatment. HS promoted periodontal bone loss and OTM and was associated with reduced bone density. Deletion of NFAT-5 led to increased osteoclast activity with NS, whereas we detected impaired OTM in mice. Dietary salt uptake seems to accelerate OTM and induce periodontal bone loss due to reduced bone density, which may be attributed to enhanced osteoclast activity. NFAT-5 influences this reaction to HS, as we detected impaired OTM and osteoclast activity upon deletion. MDPI 2021-01-09 /pmc/articles/PMC7827744/ /pubmed/33435280 http://dx.doi.org/10.3390/ijms22020596 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schröder, Agnes
Gubernator, Joshua
Leikam, Alexandra
Nazet, Ute
Cieplik, Fabian
Jantsch, Jonathan
Neubert, Patrick
Titze, Jens
Proff, Peter
Kirschneck, Christian
Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity
title Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity
title_full Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity
title_fullStr Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity
title_full_unstemmed Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity
title_short Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity
title_sort dietary salt accelerates orthodontic tooth movement by increased osteoclast activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827744/
https://www.ncbi.nlm.nih.gov/pubmed/33435280
http://dx.doi.org/10.3390/ijms22020596
work_keys_str_mv AT schroderagnes dietarysaltacceleratesorthodontictoothmovementbyincreasedosteoclastactivity
AT gubernatorjoshua dietarysaltacceleratesorthodontictoothmovementbyincreasedosteoclastactivity
AT leikamalexandra dietarysaltacceleratesorthodontictoothmovementbyincreasedosteoclastactivity
AT nazetute dietarysaltacceleratesorthodontictoothmovementbyincreasedosteoclastactivity
AT cieplikfabian dietarysaltacceleratesorthodontictoothmovementbyincreasedosteoclastactivity
AT jantschjonathan dietarysaltacceleratesorthodontictoothmovementbyincreasedosteoclastactivity
AT neubertpatrick dietarysaltacceleratesorthodontictoothmovementbyincreasedosteoclastactivity
AT titzejens dietarysaltacceleratesorthodontictoothmovementbyincreasedosteoclastactivity
AT proffpeter dietarysaltacceleratesorthodontictoothmovementbyincreasedosteoclastactivity
AT kirschneckchristian dietarysaltacceleratesorthodontictoothmovementbyincreasedosteoclastactivity