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Simultaneous Oxidation of Atmospheric Methane, Carbon Monoxide and Hydrogen for Bacterial Growth

The second largest sink for atmospheric methane (CH(4)) is atmospheric methane oxidizing-bacteria (atmMOB). How atmMOB are able to sustain life on the low CH(4) concentrations in air is unknown. Here, we show that during growth, with air as its only source for energy and carbon, the recently isolate...

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Autores principales: Tveit, Alexander Tøsdal, Schmider, Tilman, Hestnes, Anne Grethe, Lindgren, Matteus, Didriksen, Alena, Svenning, Mette Marianne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827875/
https://www.ncbi.nlm.nih.gov/pubmed/33445466
http://dx.doi.org/10.3390/microorganisms9010153
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author Tveit, Alexander Tøsdal
Schmider, Tilman
Hestnes, Anne Grethe
Lindgren, Matteus
Didriksen, Alena
Svenning, Mette Marianne
author_facet Tveit, Alexander Tøsdal
Schmider, Tilman
Hestnes, Anne Grethe
Lindgren, Matteus
Didriksen, Alena
Svenning, Mette Marianne
author_sort Tveit, Alexander Tøsdal
collection PubMed
description The second largest sink for atmospheric methane (CH(4)) is atmospheric methane oxidizing-bacteria (atmMOB). How atmMOB are able to sustain life on the low CH(4) concentrations in air is unknown. Here, we show that during growth, with air as its only source for energy and carbon, the recently isolated atmospheric methane-oxidizer Methylocapsa gorgona MG08 (USCα) oxidizes three atmospheric energy sources: CH(4), carbon monoxide (CO), and hydrogen (H(2)) to support growth. The cell-specific CH(4) oxidation rate of M. gorgona MG08 was estimated at ~0.7 × 10(−18) mol cell(−1) h(−1), which, together with the oxidation of CO and H(2), supplies 0.38 kJ Cmol(−1) h(−1) during growth in air. This is seven times lower than previously assumed necessary to support bacterial maintenance. We conclude that atmospheric methane-oxidation is supported by a metabolic flexibility that enables the simultaneous harvest of CH(4), H(2) and CO from air, but the key characteristic of atmospheric CH(4) oxidizing bacteria might be very low energy requirements.
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spelling pubmed-78278752021-01-25 Simultaneous Oxidation of Atmospheric Methane, Carbon Monoxide and Hydrogen for Bacterial Growth Tveit, Alexander Tøsdal Schmider, Tilman Hestnes, Anne Grethe Lindgren, Matteus Didriksen, Alena Svenning, Mette Marianne Microorganisms Article The second largest sink for atmospheric methane (CH(4)) is atmospheric methane oxidizing-bacteria (atmMOB). How atmMOB are able to sustain life on the low CH(4) concentrations in air is unknown. Here, we show that during growth, with air as its only source for energy and carbon, the recently isolated atmospheric methane-oxidizer Methylocapsa gorgona MG08 (USCα) oxidizes three atmospheric energy sources: CH(4), carbon monoxide (CO), and hydrogen (H(2)) to support growth. The cell-specific CH(4) oxidation rate of M. gorgona MG08 was estimated at ~0.7 × 10(−18) mol cell(−1) h(−1), which, together with the oxidation of CO and H(2), supplies 0.38 kJ Cmol(−1) h(−1) during growth in air. This is seven times lower than previously assumed necessary to support bacterial maintenance. We conclude that atmospheric methane-oxidation is supported by a metabolic flexibility that enables the simultaneous harvest of CH(4), H(2) and CO from air, but the key characteristic of atmospheric CH(4) oxidizing bacteria might be very low energy requirements. MDPI 2021-01-12 /pmc/articles/PMC7827875/ /pubmed/33445466 http://dx.doi.org/10.3390/microorganisms9010153 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tveit, Alexander Tøsdal
Schmider, Tilman
Hestnes, Anne Grethe
Lindgren, Matteus
Didriksen, Alena
Svenning, Mette Marianne
Simultaneous Oxidation of Atmospheric Methane, Carbon Monoxide and Hydrogen for Bacterial Growth
title Simultaneous Oxidation of Atmospheric Methane, Carbon Monoxide and Hydrogen for Bacterial Growth
title_full Simultaneous Oxidation of Atmospheric Methane, Carbon Monoxide and Hydrogen for Bacterial Growth
title_fullStr Simultaneous Oxidation of Atmospheric Methane, Carbon Monoxide and Hydrogen for Bacterial Growth
title_full_unstemmed Simultaneous Oxidation of Atmospheric Methane, Carbon Monoxide and Hydrogen for Bacterial Growth
title_short Simultaneous Oxidation of Atmospheric Methane, Carbon Monoxide and Hydrogen for Bacterial Growth
title_sort simultaneous oxidation of atmospheric methane, carbon monoxide and hydrogen for bacterial growth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827875/
https://www.ncbi.nlm.nih.gov/pubmed/33445466
http://dx.doi.org/10.3390/microorganisms9010153
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