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Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis

Autoimmune hepatitis (AIH) is detected at a late stage in the course of the disease. Therefore, induction and etiology are largely unclear. It is controversial if the induction of autoimmunity occurs in the liver or in the spleen. In our experimental murine AIH model, the induction of autoimmunity d...

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Autores principales: Buitrago-Molina, Laura Elisa, Dywicki, Janine, Noyan, Fatih, Trippler, Martin, Pietrek, Julia, Schlue, Jerome, Manns, Michael P., Wedemeyer, Heiner, Jaeckel, Elmar, Hardtke-Wolenski, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827897/
https://www.ncbi.nlm.nih.gov/pubmed/33435354
http://dx.doi.org/10.3390/biomedicines9010058
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author Buitrago-Molina, Laura Elisa
Dywicki, Janine
Noyan, Fatih
Trippler, Martin
Pietrek, Julia
Schlue, Jerome
Manns, Michael P.
Wedemeyer, Heiner
Jaeckel, Elmar
Hardtke-Wolenski, Matthias
author_facet Buitrago-Molina, Laura Elisa
Dywicki, Janine
Noyan, Fatih
Trippler, Martin
Pietrek, Julia
Schlue, Jerome
Manns, Michael P.
Wedemeyer, Heiner
Jaeckel, Elmar
Hardtke-Wolenski, Matthias
author_sort Buitrago-Molina, Laura Elisa
collection PubMed
description Autoimmune hepatitis (AIH) is detected at a late stage in the course of the disease. Therefore, induction and etiology are largely unclear. It is controversial if the induction of autoimmunity occurs in the liver or in the spleen. In our experimental murine AIH model, the induction of autoimmunity did not occur in the spleen. Instead, a protective role of the spleen could be more likely. Therefore, we splenectomized mice followed by induction of experimental murine AIH. Splenectomized mice presented more severe portal inflammation. Furthermore, these mice had more IL-17, IL-23 receptor (IL-23R) and caspase 3 (casp3) and a decreased amount of erythropoietin in serum, while intrahepatic T cell compartments were unaffected. These results indicate that the spleen is not necessary for induction of AIH, and splenectomy disrupts the ability to immune regulate the intensity of hepatic inflammation, production of IL-17 and apoptosis.
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spelling pubmed-78278972021-01-25 Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis Buitrago-Molina, Laura Elisa Dywicki, Janine Noyan, Fatih Trippler, Martin Pietrek, Julia Schlue, Jerome Manns, Michael P. Wedemeyer, Heiner Jaeckel, Elmar Hardtke-Wolenski, Matthias Biomedicines Communication Autoimmune hepatitis (AIH) is detected at a late stage in the course of the disease. Therefore, induction and etiology are largely unclear. It is controversial if the induction of autoimmunity occurs in the liver or in the spleen. In our experimental murine AIH model, the induction of autoimmunity did not occur in the spleen. Instead, a protective role of the spleen could be more likely. Therefore, we splenectomized mice followed by induction of experimental murine AIH. Splenectomized mice presented more severe portal inflammation. Furthermore, these mice had more IL-17, IL-23 receptor (IL-23R) and caspase 3 (casp3) and a decreased amount of erythropoietin in serum, while intrahepatic T cell compartments were unaffected. These results indicate that the spleen is not necessary for induction of AIH, and splenectomy disrupts the ability to immune regulate the intensity of hepatic inflammation, production of IL-17 and apoptosis. MDPI 2021-01-09 /pmc/articles/PMC7827897/ /pubmed/33435354 http://dx.doi.org/10.3390/biomedicines9010058 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Buitrago-Molina, Laura Elisa
Dywicki, Janine
Noyan, Fatih
Trippler, Martin
Pietrek, Julia
Schlue, Jerome
Manns, Michael P.
Wedemeyer, Heiner
Jaeckel, Elmar
Hardtke-Wolenski, Matthias
Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis
title Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis
title_full Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis
title_fullStr Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis
title_full_unstemmed Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis
title_short Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis
title_sort splenectomy prior to experimental induction of autoimmune hepatitis promotes more severe hepatic inflammation, production of il-17 and apoptosis
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827897/
https://www.ncbi.nlm.nih.gov/pubmed/33435354
http://dx.doi.org/10.3390/biomedicines9010058
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