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Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis
Autoimmune hepatitis (AIH) is detected at a late stage in the course of the disease. Therefore, induction and etiology are largely unclear. It is controversial if the induction of autoimmunity occurs in the liver or in the spleen. In our experimental murine AIH model, the induction of autoimmunity d...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827897/ https://www.ncbi.nlm.nih.gov/pubmed/33435354 http://dx.doi.org/10.3390/biomedicines9010058 |
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author | Buitrago-Molina, Laura Elisa Dywicki, Janine Noyan, Fatih Trippler, Martin Pietrek, Julia Schlue, Jerome Manns, Michael P. Wedemeyer, Heiner Jaeckel, Elmar Hardtke-Wolenski, Matthias |
author_facet | Buitrago-Molina, Laura Elisa Dywicki, Janine Noyan, Fatih Trippler, Martin Pietrek, Julia Schlue, Jerome Manns, Michael P. Wedemeyer, Heiner Jaeckel, Elmar Hardtke-Wolenski, Matthias |
author_sort | Buitrago-Molina, Laura Elisa |
collection | PubMed |
description | Autoimmune hepatitis (AIH) is detected at a late stage in the course of the disease. Therefore, induction and etiology are largely unclear. It is controversial if the induction of autoimmunity occurs in the liver or in the spleen. In our experimental murine AIH model, the induction of autoimmunity did not occur in the spleen. Instead, a protective role of the spleen could be more likely. Therefore, we splenectomized mice followed by induction of experimental murine AIH. Splenectomized mice presented more severe portal inflammation. Furthermore, these mice had more IL-17, IL-23 receptor (IL-23R) and caspase 3 (casp3) and a decreased amount of erythropoietin in serum, while intrahepatic T cell compartments were unaffected. These results indicate that the spleen is not necessary for induction of AIH, and splenectomy disrupts the ability to immune regulate the intensity of hepatic inflammation, production of IL-17 and apoptosis. |
format | Online Article Text |
id | pubmed-7827897 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78278972021-01-25 Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis Buitrago-Molina, Laura Elisa Dywicki, Janine Noyan, Fatih Trippler, Martin Pietrek, Julia Schlue, Jerome Manns, Michael P. Wedemeyer, Heiner Jaeckel, Elmar Hardtke-Wolenski, Matthias Biomedicines Communication Autoimmune hepatitis (AIH) is detected at a late stage in the course of the disease. Therefore, induction and etiology are largely unclear. It is controversial if the induction of autoimmunity occurs in the liver or in the spleen. In our experimental murine AIH model, the induction of autoimmunity did not occur in the spleen. Instead, a protective role of the spleen could be more likely. Therefore, we splenectomized mice followed by induction of experimental murine AIH. Splenectomized mice presented more severe portal inflammation. Furthermore, these mice had more IL-17, IL-23 receptor (IL-23R) and caspase 3 (casp3) and a decreased amount of erythropoietin in serum, while intrahepatic T cell compartments were unaffected. These results indicate that the spleen is not necessary for induction of AIH, and splenectomy disrupts the ability to immune regulate the intensity of hepatic inflammation, production of IL-17 and apoptosis. MDPI 2021-01-09 /pmc/articles/PMC7827897/ /pubmed/33435354 http://dx.doi.org/10.3390/biomedicines9010058 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Buitrago-Molina, Laura Elisa Dywicki, Janine Noyan, Fatih Trippler, Martin Pietrek, Julia Schlue, Jerome Manns, Michael P. Wedemeyer, Heiner Jaeckel, Elmar Hardtke-Wolenski, Matthias Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis |
title | Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis |
title_full | Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis |
title_fullStr | Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis |
title_full_unstemmed | Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis |
title_short | Splenectomy Prior to Experimental Induction of Autoimmune Hepatitis Promotes More Severe Hepatic Inflammation, Production of IL-17 and Apoptosis |
title_sort | splenectomy prior to experimental induction of autoimmune hepatitis promotes more severe hepatic inflammation, production of il-17 and apoptosis |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827897/ https://www.ncbi.nlm.nih.gov/pubmed/33435354 http://dx.doi.org/10.3390/biomedicines9010058 |
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