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Gynura procumbens Root Extract Ameliorates Ischemia-Induced Neuronal Damage in the Hippocampal CA1 Region by Reducing Neuroinflammation

Gynura procumbens has been used in Southeast Asia for the treatment of hypertension, hyperglycemia, and skin problems induced by ultraviolet irradiation. Although considerable studies have reported the biological properties of Gynura procumbens root extract (GPE-R), there are no studies on the effec...

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Autores principales: Kim, Woosuk, Jung, Hyo Young, Yoo, Dae Young, Kwon, Hyun Jung, Hahn, Kyu Ri, Kim, Dae Won, Yoon, Yeo Sung, Choi, Soo Young, Hwang, In Koo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828071/
https://www.ncbi.nlm.nih.gov/pubmed/33435613
http://dx.doi.org/10.3390/nu13010181
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author Kim, Woosuk
Jung, Hyo Young
Yoo, Dae Young
Kwon, Hyun Jung
Hahn, Kyu Ri
Kim, Dae Won
Yoon, Yeo Sung
Choi, Soo Young
Hwang, In Koo
author_facet Kim, Woosuk
Jung, Hyo Young
Yoo, Dae Young
Kwon, Hyun Jung
Hahn, Kyu Ri
Kim, Dae Won
Yoon, Yeo Sung
Choi, Soo Young
Hwang, In Koo
author_sort Kim, Woosuk
collection PubMed
description Gynura procumbens has been used in Southeast Asia for the treatment of hypertension, hyperglycemia, and skin problems induced by ultraviolet irradiation. Although considerable studies have reported the biological properties of Gynura procumbens root extract (GPE-R), there are no studies on the effects of GPE-R in brain damages, for example following brain ischemia. In the present study, we screened the neuroprotective effects of GPE-R against ischemic damage and neuroinflammation in the hippocampus based on behavioral, morphological, and biological approaches. Gerbils received oral administration of GPE-R (30 and 300 mg/kg) every day for three weeks and 2 h after the last administration, ischemic surgery was done by occlusion of both common carotid arteries for 5 min. Administration of 300 mg/kg GPE-R significantly reduced ischemia-induced locomotor hyperactivity 1 day after ischemia. Significantly more NeuN-positive neurons were observed in the hippocampal CA1 regions of 300 mg/kg GPE-R-treated animals compared to those in the vehicle-treated group 4 days after ischemia. Administration of GPE-R significantly reduced levels of pro-inflammatory cytokines such as interleukin-1β, -6, and tumor necrosis factor-α 6 h after ischemia/reperfusion. In addition, activated microglia were significantly decreased in the 300 mg/kg GPE-R-treated group four days after ischemia/reperfusion compared to the vehicle-treated group. These results suggest that GPE-R may be one of the possible agents to protect neurons from ischemic damage by reducing inflammatory responses.
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spelling pubmed-78280712021-01-25 Gynura procumbens Root Extract Ameliorates Ischemia-Induced Neuronal Damage in the Hippocampal CA1 Region by Reducing Neuroinflammation Kim, Woosuk Jung, Hyo Young Yoo, Dae Young Kwon, Hyun Jung Hahn, Kyu Ri Kim, Dae Won Yoon, Yeo Sung Choi, Soo Young Hwang, In Koo Nutrients Article Gynura procumbens has been used in Southeast Asia for the treatment of hypertension, hyperglycemia, and skin problems induced by ultraviolet irradiation. Although considerable studies have reported the biological properties of Gynura procumbens root extract (GPE-R), there are no studies on the effects of GPE-R in brain damages, for example following brain ischemia. In the present study, we screened the neuroprotective effects of GPE-R against ischemic damage and neuroinflammation in the hippocampus based on behavioral, morphological, and biological approaches. Gerbils received oral administration of GPE-R (30 and 300 mg/kg) every day for three weeks and 2 h after the last administration, ischemic surgery was done by occlusion of both common carotid arteries for 5 min. Administration of 300 mg/kg GPE-R significantly reduced ischemia-induced locomotor hyperactivity 1 day after ischemia. Significantly more NeuN-positive neurons were observed in the hippocampal CA1 regions of 300 mg/kg GPE-R-treated animals compared to those in the vehicle-treated group 4 days after ischemia. Administration of GPE-R significantly reduced levels of pro-inflammatory cytokines such as interleukin-1β, -6, and tumor necrosis factor-α 6 h after ischemia/reperfusion. In addition, activated microglia were significantly decreased in the 300 mg/kg GPE-R-treated group four days after ischemia/reperfusion compared to the vehicle-treated group. These results suggest that GPE-R may be one of the possible agents to protect neurons from ischemic damage by reducing inflammatory responses. MDPI 2021-01-08 /pmc/articles/PMC7828071/ /pubmed/33435613 http://dx.doi.org/10.3390/nu13010181 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Woosuk
Jung, Hyo Young
Yoo, Dae Young
Kwon, Hyun Jung
Hahn, Kyu Ri
Kim, Dae Won
Yoon, Yeo Sung
Choi, Soo Young
Hwang, In Koo
Gynura procumbens Root Extract Ameliorates Ischemia-Induced Neuronal Damage in the Hippocampal CA1 Region by Reducing Neuroinflammation
title Gynura procumbens Root Extract Ameliorates Ischemia-Induced Neuronal Damage in the Hippocampal CA1 Region by Reducing Neuroinflammation
title_full Gynura procumbens Root Extract Ameliorates Ischemia-Induced Neuronal Damage in the Hippocampal CA1 Region by Reducing Neuroinflammation
title_fullStr Gynura procumbens Root Extract Ameliorates Ischemia-Induced Neuronal Damage in the Hippocampal CA1 Region by Reducing Neuroinflammation
title_full_unstemmed Gynura procumbens Root Extract Ameliorates Ischemia-Induced Neuronal Damage in the Hippocampal CA1 Region by Reducing Neuroinflammation
title_short Gynura procumbens Root Extract Ameliorates Ischemia-Induced Neuronal Damage in the Hippocampal CA1 Region by Reducing Neuroinflammation
title_sort gynura procumbens root extract ameliorates ischemia-induced neuronal damage in the hippocampal ca1 region by reducing neuroinflammation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828071/
https://www.ncbi.nlm.nih.gov/pubmed/33435613
http://dx.doi.org/10.3390/nu13010181
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