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Shear Stress and RBC-NOS Serine1177 Phosphorylation in Humans: A Dose Response
Red blood cells (RBC) express a nitric oxide synthase isoform (RBC-NOS) that appears dependent on shear stress for Serine1177 phosphorylation. Whether this protein is equally activated by varied shears in the physiological range is less described. Here, we explored RBC-NOS Serine1177 phosphorylation...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828091/ https://www.ncbi.nlm.nih.gov/pubmed/33429979 http://dx.doi.org/10.3390/life11010036 |
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author | Horobin, Jarod T. Sabapathy, Surendran Kuck, Lennart Simmonds, Michael J. |
author_facet | Horobin, Jarod T. Sabapathy, Surendran Kuck, Lennart Simmonds, Michael J. |
author_sort | Horobin, Jarod T. |
collection | PubMed |
description | Red blood cells (RBC) express a nitric oxide synthase isoform (RBC-NOS) that appears dependent on shear stress for Serine1177 phosphorylation. Whether this protein is equally activated by varied shears in the physiological range is less described. Here, we explored RBC-NOS Serine1177 phosphorylation in response to shear stress levels reflective of in vivo conditions. Whole blood samples were exposed to specific magnitudes of shear stress (0.5, 1.5, 4.5, 13.5 Pa) for discrete exposure times (1, 10, 30 min). Thereafter, RBC-NOS Serine1177 phosphorylation was measured utilising immunofluorescence labelling. Shear stress exposure at 0.5, 1.5, and 13.5 Pa significantly increased RBC-NOS Serine1177 phosphorylation following 1 min (p < 0.0001); exposure to 4.5 Pa had no effect after 1 min. RBC-NOS Serine1177 phosphorylation was significantly increased following 10 min at each magnitude of shear stress (0.5, 1.5, 13.5 Pa, p < 0.0001; 4.5 Pa, p = 0.0042). Shear stress exposure for 30 min significantly increased RBC-NOS Serine1177 phosphorylation at 0.5 Pa and 13.5 Pa (p < 0.0001). We found that RBC-NOS phosphorylation via shear stress is non-linear and differs for a given magnitude and duration of exposure. This study provides a new understanding of the discrete relation between RBC-NOS and shear stress. |
format | Online Article Text |
id | pubmed-7828091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78280912021-01-25 Shear Stress and RBC-NOS Serine1177 Phosphorylation in Humans: A Dose Response Horobin, Jarod T. Sabapathy, Surendran Kuck, Lennart Simmonds, Michael J. Life (Basel) Article Red blood cells (RBC) express a nitric oxide synthase isoform (RBC-NOS) that appears dependent on shear stress for Serine1177 phosphorylation. Whether this protein is equally activated by varied shears in the physiological range is less described. Here, we explored RBC-NOS Serine1177 phosphorylation in response to shear stress levels reflective of in vivo conditions. Whole blood samples were exposed to specific magnitudes of shear stress (0.5, 1.5, 4.5, 13.5 Pa) for discrete exposure times (1, 10, 30 min). Thereafter, RBC-NOS Serine1177 phosphorylation was measured utilising immunofluorescence labelling. Shear stress exposure at 0.5, 1.5, and 13.5 Pa significantly increased RBC-NOS Serine1177 phosphorylation following 1 min (p < 0.0001); exposure to 4.5 Pa had no effect after 1 min. RBC-NOS Serine1177 phosphorylation was significantly increased following 10 min at each magnitude of shear stress (0.5, 1.5, 13.5 Pa, p < 0.0001; 4.5 Pa, p = 0.0042). Shear stress exposure for 30 min significantly increased RBC-NOS Serine1177 phosphorylation at 0.5 Pa and 13.5 Pa (p < 0.0001). We found that RBC-NOS phosphorylation via shear stress is non-linear and differs for a given magnitude and duration of exposure. This study provides a new understanding of the discrete relation between RBC-NOS and shear stress. MDPI 2021-01-08 /pmc/articles/PMC7828091/ /pubmed/33429979 http://dx.doi.org/10.3390/life11010036 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Horobin, Jarod T. Sabapathy, Surendran Kuck, Lennart Simmonds, Michael J. Shear Stress and RBC-NOS Serine1177 Phosphorylation in Humans: A Dose Response |
title | Shear Stress and RBC-NOS Serine1177 Phosphorylation in Humans: A Dose Response |
title_full | Shear Stress and RBC-NOS Serine1177 Phosphorylation in Humans: A Dose Response |
title_fullStr | Shear Stress and RBC-NOS Serine1177 Phosphorylation in Humans: A Dose Response |
title_full_unstemmed | Shear Stress and RBC-NOS Serine1177 Phosphorylation in Humans: A Dose Response |
title_short | Shear Stress and RBC-NOS Serine1177 Phosphorylation in Humans: A Dose Response |
title_sort | shear stress and rbc-nos serine1177 phosphorylation in humans: a dose response |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828091/ https://www.ncbi.nlm.nih.gov/pubmed/33429979 http://dx.doi.org/10.3390/life11010036 |
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