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Lipocalin-2 Deficiency Reduces Oxidative Stress and Neuroinflammation and Results in Attenuation of Kainic Acid-Induced Hippocampal Cell Death

The hippocampal cell death that follows kainic acid (KA)-induced seizures is associated with blood–brain barrier (BBB) leakage and oxidative stress. Lipocalin-2 (LCN2) is an iron-trafficking protein which contributes to both oxidative stress and inflammation. However, LCN2′s role in KA-induced hippo...

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Autores principales: Shin, Hyun Joo, Jeong, Eun Ae, Lee, Jong Youl, An, Hyeong Seok, Jang, Hye Min, Ahn, Yu Jeong, Lee, Jaewoong, Kim, Kyung Eun, Roh, Gu Seob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828212/
https://www.ncbi.nlm.nih.gov/pubmed/33445746
http://dx.doi.org/10.3390/antiox10010100
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author Shin, Hyun Joo
Jeong, Eun Ae
Lee, Jong Youl
An, Hyeong Seok
Jang, Hye Min
Ahn, Yu Jeong
Lee, Jaewoong
Kim, Kyung Eun
Roh, Gu Seob
author_facet Shin, Hyun Joo
Jeong, Eun Ae
Lee, Jong Youl
An, Hyeong Seok
Jang, Hye Min
Ahn, Yu Jeong
Lee, Jaewoong
Kim, Kyung Eun
Roh, Gu Seob
author_sort Shin, Hyun Joo
collection PubMed
description The hippocampal cell death that follows kainic acid (KA)-induced seizures is associated with blood–brain barrier (BBB) leakage and oxidative stress. Lipocalin-2 (LCN2) is an iron-trafficking protein which contributes to both oxidative stress and inflammation. However, LCN2′s role in KA-induced hippocampal cell death is not clear. Here, we examine the effect of blocking LCN2 genetically on neuroinflammation and oxidative stress in KA-induced neuronal death. LCN2 deficiency reduced neuronal cell death and BBB leakage in the KA-treated hippocampus. In addition to LCN2 upregulation in the KA-treated hippocampus, circulating LCN2 levels were significantly increased in KA-treated wild-type (WT) mice. In LCN2 knockout mice, we found that the expressions of neutrophil markers myeloperoxidase and neutrophil elastase were decreased compared to their expressions in WT mice following KA treatment. Furthermore, LCN2 deficiency also attenuated KA-induced iron overload and oxidative stress in the hippocampus. These findings indicate that LCN2 may play an important role in iron-related oxidative stress and neuroinflammation in KA-induced hippocampal cell death.
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spelling pubmed-78282122021-01-25 Lipocalin-2 Deficiency Reduces Oxidative Stress and Neuroinflammation and Results in Attenuation of Kainic Acid-Induced Hippocampal Cell Death Shin, Hyun Joo Jeong, Eun Ae Lee, Jong Youl An, Hyeong Seok Jang, Hye Min Ahn, Yu Jeong Lee, Jaewoong Kim, Kyung Eun Roh, Gu Seob Antioxidants (Basel) Article The hippocampal cell death that follows kainic acid (KA)-induced seizures is associated with blood–brain barrier (BBB) leakage and oxidative stress. Lipocalin-2 (LCN2) is an iron-trafficking protein which contributes to both oxidative stress and inflammation. However, LCN2′s role in KA-induced hippocampal cell death is not clear. Here, we examine the effect of blocking LCN2 genetically on neuroinflammation and oxidative stress in KA-induced neuronal death. LCN2 deficiency reduced neuronal cell death and BBB leakage in the KA-treated hippocampus. In addition to LCN2 upregulation in the KA-treated hippocampus, circulating LCN2 levels were significantly increased in KA-treated wild-type (WT) mice. In LCN2 knockout mice, we found that the expressions of neutrophil markers myeloperoxidase and neutrophil elastase were decreased compared to their expressions in WT mice following KA treatment. Furthermore, LCN2 deficiency also attenuated KA-induced iron overload and oxidative stress in the hippocampus. These findings indicate that LCN2 may play an important role in iron-related oxidative stress and neuroinflammation in KA-induced hippocampal cell death. MDPI 2021-01-12 /pmc/articles/PMC7828212/ /pubmed/33445746 http://dx.doi.org/10.3390/antiox10010100 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shin, Hyun Joo
Jeong, Eun Ae
Lee, Jong Youl
An, Hyeong Seok
Jang, Hye Min
Ahn, Yu Jeong
Lee, Jaewoong
Kim, Kyung Eun
Roh, Gu Seob
Lipocalin-2 Deficiency Reduces Oxidative Stress and Neuroinflammation and Results in Attenuation of Kainic Acid-Induced Hippocampal Cell Death
title Lipocalin-2 Deficiency Reduces Oxidative Stress and Neuroinflammation and Results in Attenuation of Kainic Acid-Induced Hippocampal Cell Death
title_full Lipocalin-2 Deficiency Reduces Oxidative Stress and Neuroinflammation and Results in Attenuation of Kainic Acid-Induced Hippocampal Cell Death
title_fullStr Lipocalin-2 Deficiency Reduces Oxidative Stress and Neuroinflammation and Results in Attenuation of Kainic Acid-Induced Hippocampal Cell Death
title_full_unstemmed Lipocalin-2 Deficiency Reduces Oxidative Stress and Neuroinflammation and Results in Attenuation of Kainic Acid-Induced Hippocampal Cell Death
title_short Lipocalin-2 Deficiency Reduces Oxidative Stress and Neuroinflammation and Results in Attenuation of Kainic Acid-Induced Hippocampal Cell Death
title_sort lipocalin-2 deficiency reduces oxidative stress and neuroinflammation and results in attenuation of kainic acid-induced hippocampal cell death
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828212/
https://www.ncbi.nlm.nih.gov/pubmed/33445746
http://dx.doi.org/10.3390/antiox10010100
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