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Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study

MicroRNAs (miRNAs) are small noncoding RNAs that have emerged as new potential epigenetic biomarkers. Here, we evaluate the efficacy of six circulating miRNA previously described in the literature as biomarkers for the diagnosis of temporal lobe epilepsy (TLE) and/or as predictive biomarkers to anti...

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Autores principales: De Benedittis, Selene, Fortunato, Francesco, Cava, Claudia, Gallivanone, Francesca, Iaccino, Enrico, Caligiuri, Maria Eugenia, Castiglioni, Isabella, Bertoli, Gloria, Manna, Ida, Labate, Angelo, Gambardella, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828221/
https://www.ncbi.nlm.nih.gov/pubmed/33445780
http://dx.doi.org/10.3390/ijms22020702
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author De Benedittis, Selene
Fortunato, Francesco
Cava, Claudia
Gallivanone, Francesca
Iaccino, Enrico
Caligiuri, Maria Eugenia
Castiglioni, Isabella
Bertoli, Gloria
Manna, Ida
Labate, Angelo
Gambardella, Antonio
author_facet De Benedittis, Selene
Fortunato, Francesco
Cava, Claudia
Gallivanone, Francesca
Iaccino, Enrico
Caligiuri, Maria Eugenia
Castiglioni, Isabella
Bertoli, Gloria
Manna, Ida
Labate, Angelo
Gambardella, Antonio
author_sort De Benedittis, Selene
collection PubMed
description MicroRNAs (miRNAs) are small noncoding RNAs that have emerged as new potential epigenetic biomarkers. Here, we evaluate the efficacy of six circulating miRNA previously described in the literature as biomarkers for the diagnosis of temporal lobe epilepsy (TLE) and/or as predictive biomarkers to antiepileptic drug response. We measured the differences in serum miRNA levels by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays in a cohort of 27 patients (14 women and 13 men; mean ± SD age: 43.65 ± 17.07) with TLE compared to 20 healthy controls (HC) matched for sex, age and ethnicity (11 women and 9 men; mean ± SD age: 47.5 ± 9.1). Additionally, patients were classified according to whether they had drug-responsive (n = 17) or drug-resistant (n = 10) TLE. We have investigated any correlations between miRNAs and several electroclinical parameters. Three miRNAs (miR-142, miR-146a, miR-223) were significantly upregulated in patients (expressed as average expression ± SD). In detail, miR-142 expression was 0.40 ± 0.29 vs. 0.16 ± 0.10 in TLE patients compared to HC (t-test, p < 0.01), miR-146a expression was 0.15 ± 0.11 vs. 0.07 ± 0.04 (t-test, p < 0.05), and miR-223 expression was 6.21 ± 3.65 vs. 1.23 ± 0.84 (t-test, p < 0.001). Moreover, results obtained from a logistic regression model showed the good performance of miR-142 and miR-223 in distinguishing drug-sensitive vs. drug-resistant TLE. The results of this pilot study give evidence that miRNAs are suitable targets in TLE and offer the rationale for further confirmation studies in larger epilepsy cohorts.
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spelling pubmed-78282212021-01-25 Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study De Benedittis, Selene Fortunato, Francesco Cava, Claudia Gallivanone, Francesca Iaccino, Enrico Caligiuri, Maria Eugenia Castiglioni, Isabella Bertoli, Gloria Manna, Ida Labate, Angelo Gambardella, Antonio Int J Mol Sci Article MicroRNAs (miRNAs) are small noncoding RNAs that have emerged as new potential epigenetic biomarkers. Here, we evaluate the efficacy of six circulating miRNA previously described in the literature as biomarkers for the diagnosis of temporal lobe epilepsy (TLE) and/or as predictive biomarkers to antiepileptic drug response. We measured the differences in serum miRNA levels by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays in a cohort of 27 patients (14 women and 13 men; mean ± SD age: 43.65 ± 17.07) with TLE compared to 20 healthy controls (HC) matched for sex, age and ethnicity (11 women and 9 men; mean ± SD age: 47.5 ± 9.1). Additionally, patients were classified according to whether they had drug-responsive (n = 17) or drug-resistant (n = 10) TLE. We have investigated any correlations between miRNAs and several electroclinical parameters. Three miRNAs (miR-142, miR-146a, miR-223) were significantly upregulated in patients (expressed as average expression ± SD). In detail, miR-142 expression was 0.40 ± 0.29 vs. 0.16 ± 0.10 in TLE patients compared to HC (t-test, p < 0.01), miR-146a expression was 0.15 ± 0.11 vs. 0.07 ± 0.04 (t-test, p < 0.05), and miR-223 expression was 6.21 ± 3.65 vs. 1.23 ± 0.84 (t-test, p < 0.001). Moreover, results obtained from a logistic regression model showed the good performance of miR-142 and miR-223 in distinguishing drug-sensitive vs. drug-resistant TLE. The results of this pilot study give evidence that miRNAs are suitable targets in TLE and offer the rationale for further confirmation studies in larger epilepsy cohorts. MDPI 2021-01-12 /pmc/articles/PMC7828221/ /pubmed/33445780 http://dx.doi.org/10.3390/ijms22020702 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
De Benedittis, Selene
Fortunato, Francesco
Cava, Claudia
Gallivanone, Francesca
Iaccino, Enrico
Caligiuri, Maria Eugenia
Castiglioni, Isabella
Bertoli, Gloria
Manna, Ida
Labate, Angelo
Gambardella, Antonio
Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study
title Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study
title_full Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study
title_fullStr Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study
title_full_unstemmed Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study
title_short Circulating microRNAs as Potential Novel Diagnostic Biomarkers to Predict Drug Resistance in Temporal Lobe Epilepsy: A Pilot Study
title_sort circulating micrornas as potential novel diagnostic biomarkers to predict drug resistance in temporal lobe epilepsy: a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828221/
https://www.ncbi.nlm.nih.gov/pubmed/33445780
http://dx.doi.org/10.3390/ijms22020702
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