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Annurca Apple Polyphenol Extract Affects Acetyl- Cholinesterase and Mono-Amine Oxidase In Vitro Enzyme Activity
In this study, we explored the ability of Annurca apple flesh polyphenol extract (AFPE) to affect the activity of key enzymes involved in neurodegenerative disorders—in particular, Acetyl- and Butirryl-cholinesterases, and type A and B monoamine oxidase. The effect of AFPE on enzyme activity was ana...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828649/ https://www.ncbi.nlm.nih.gov/pubmed/33466604 http://dx.doi.org/10.3390/ph14010062 |
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author | Nasso, Rosarita Pagliara, Valentina D’Angelo, Stefania Rullo, Rosario Masullo, Mariorosario Arcone, Rosaria |
author_facet | Nasso, Rosarita Pagliara, Valentina D’Angelo, Stefania Rullo, Rosario Masullo, Mariorosario Arcone, Rosaria |
author_sort | Nasso, Rosarita |
collection | PubMed |
description | In this study, we explored the ability of Annurca apple flesh polyphenol extract (AFPE) to affect the activity of key enzymes involved in neurodegenerative disorders—in particular, Acetyl- and Butirryl-cholinesterases, and type A and B monoamine oxidase. The effect of AFPE on enzyme activity was analyzed by in vitro enzyme assays, and the results showed concentration-dependent enzyme inhibition, with IC(50) values corresponding to 859 ± 18 µM and 966 ± 72 µM for AChE and BuChE respectively, and IC(50) corresponding to 145 ± 3 µM and 199 ± 7 µM for MAO-A and MAO-B, respectively, with a preference for MAO-A. Moreover, in this concentration range, AFPE did not affect the viability of human neuroblastoma SH-SY5Y and fibroblast BJ-5ta cell lines, as determined by an MTT assay. In conclusion, our results demonstrate that AFPE shows the new biological properties of inhibiting the activity of enzymes that are involved in brain functions, neurodegenerative disorders, and aging. |
format | Online Article Text |
id | pubmed-7828649 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78286492021-01-25 Annurca Apple Polyphenol Extract Affects Acetyl- Cholinesterase and Mono-Amine Oxidase In Vitro Enzyme Activity Nasso, Rosarita Pagliara, Valentina D’Angelo, Stefania Rullo, Rosario Masullo, Mariorosario Arcone, Rosaria Pharmaceuticals (Basel) Article In this study, we explored the ability of Annurca apple flesh polyphenol extract (AFPE) to affect the activity of key enzymes involved in neurodegenerative disorders—in particular, Acetyl- and Butirryl-cholinesterases, and type A and B monoamine oxidase. The effect of AFPE on enzyme activity was analyzed by in vitro enzyme assays, and the results showed concentration-dependent enzyme inhibition, with IC(50) values corresponding to 859 ± 18 µM and 966 ± 72 µM for AChE and BuChE respectively, and IC(50) corresponding to 145 ± 3 µM and 199 ± 7 µM for MAO-A and MAO-B, respectively, with a preference for MAO-A. Moreover, in this concentration range, AFPE did not affect the viability of human neuroblastoma SH-SY5Y and fibroblast BJ-5ta cell lines, as determined by an MTT assay. In conclusion, our results demonstrate that AFPE shows the new biological properties of inhibiting the activity of enzymes that are involved in brain functions, neurodegenerative disorders, and aging. MDPI 2021-01-14 /pmc/articles/PMC7828649/ /pubmed/33466604 http://dx.doi.org/10.3390/ph14010062 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nasso, Rosarita Pagliara, Valentina D’Angelo, Stefania Rullo, Rosario Masullo, Mariorosario Arcone, Rosaria Annurca Apple Polyphenol Extract Affects Acetyl- Cholinesterase and Mono-Amine Oxidase In Vitro Enzyme Activity |
title | Annurca Apple Polyphenol Extract Affects Acetyl- Cholinesterase and Mono-Amine Oxidase In Vitro Enzyme Activity |
title_full | Annurca Apple Polyphenol Extract Affects Acetyl- Cholinesterase and Mono-Amine Oxidase In Vitro Enzyme Activity |
title_fullStr | Annurca Apple Polyphenol Extract Affects Acetyl- Cholinesterase and Mono-Amine Oxidase In Vitro Enzyme Activity |
title_full_unstemmed | Annurca Apple Polyphenol Extract Affects Acetyl- Cholinesterase and Mono-Amine Oxidase In Vitro Enzyme Activity |
title_short | Annurca Apple Polyphenol Extract Affects Acetyl- Cholinesterase and Mono-Amine Oxidase In Vitro Enzyme Activity |
title_sort | annurca apple polyphenol extract affects acetyl- cholinesterase and mono-amine oxidase in vitro enzyme activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828649/ https://www.ncbi.nlm.nih.gov/pubmed/33466604 http://dx.doi.org/10.3390/ph14010062 |
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