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Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii

This study aimed to evaluate the efficacy of amniotic fluid (AF) as an alternative to fetal bovine serum (FBS) in the maintenance of Leishmania major promastigotes and Toxoplasma gondii tachyzoites. AF was collected by an obstetrician using sterile syringes during a cesarean section. The culture med...

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Autores principales: Rahmani, Zahra, Faridnia, Roghiyeh, Kalani, Hamed, Ghanei, Naryan, Fakhar, Mahdi, Zamanian, Marzieh, Keighobadi, Masoud, Tabaripour, Rabeeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829060/
https://www.ncbi.nlm.nih.gov/pubmed/33491113
http://dx.doi.org/10.1007/s00436-021-07058-2
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author Rahmani, Zahra
Faridnia, Roghiyeh
Kalani, Hamed
Ghanei, Naryan
Fakhar, Mahdi
Zamanian, Marzieh
Keighobadi, Masoud
Tabaripour, Rabeeh
author_facet Rahmani, Zahra
Faridnia, Roghiyeh
Kalani, Hamed
Ghanei, Naryan
Fakhar, Mahdi
Zamanian, Marzieh
Keighobadi, Masoud
Tabaripour, Rabeeh
author_sort Rahmani, Zahra
collection PubMed
description This study aimed to evaluate the efficacy of amniotic fluid (AF) as an alternative to fetal bovine serum (FBS) in the maintenance of Leishmania major promastigotes and Toxoplasma gondii tachyzoites. AF was collected by an obstetrician using sterile syringes during a cesarean section. The culture medium was supplemented with 5 different concentrations of FBS or AF including 2, 5, 10, 20, and 30%. These concentrations were used to maintain both mentioned parasites. L. major was maintained at temperatures 4 and 24 °C and examined once a week for 4 weeks, while T. gondii was maintained at temperatures 4, 24, and 37 °C and examined at hours 24, 48, 72, and 96. For L. major, at both 4 and 24 °C, we observed no significant difference between FBS and AF on day 7. However, on days 14, 21, and 28, the difference between FBS and AF was significant at both temperatures. For T. gondii, no significant difference was observed between FBS and AF at hour 24 and all temperatures. However, this difference was significant at hours 48, 72, and 96 and all temperatures. According to our results, although FBS had a greater efficacy than AF in the growth of L. major and the survival of T. gondii, the number of promastigotes increased over time in AF-containing medium and the number of tachyzoites reduced slowly with a mild slop. Therefore, AF can be a potential alternative to FBS.
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spelling pubmed-78290602021-01-25 Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii Rahmani, Zahra Faridnia, Roghiyeh Kalani, Hamed Ghanei, Naryan Fakhar, Mahdi Zamanian, Marzieh Keighobadi, Masoud Tabaripour, Rabeeh Parasitol Res Protozoology - Original Paper This study aimed to evaluate the efficacy of amniotic fluid (AF) as an alternative to fetal bovine serum (FBS) in the maintenance of Leishmania major promastigotes and Toxoplasma gondii tachyzoites. AF was collected by an obstetrician using sterile syringes during a cesarean section. The culture medium was supplemented with 5 different concentrations of FBS or AF including 2, 5, 10, 20, and 30%. These concentrations were used to maintain both mentioned parasites. L. major was maintained at temperatures 4 and 24 °C and examined once a week for 4 weeks, while T. gondii was maintained at temperatures 4, 24, and 37 °C and examined at hours 24, 48, 72, and 96. For L. major, at both 4 and 24 °C, we observed no significant difference between FBS and AF on day 7. However, on days 14, 21, and 28, the difference between FBS and AF was significant at both temperatures. For T. gondii, no significant difference was observed between FBS and AF at hour 24 and all temperatures. However, this difference was significant at hours 48, 72, and 96 and all temperatures. According to our results, although FBS had a greater efficacy than AF in the growth of L. major and the survival of T. gondii, the number of promastigotes increased over time in AF-containing medium and the number of tachyzoites reduced slowly with a mild slop. Therefore, AF can be a potential alternative to FBS. Springer Berlin Heidelberg 2021-01-25 2021 /pmc/articles/PMC7829060/ /pubmed/33491113 http://dx.doi.org/10.1007/s00436-021-07058-2 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Protozoology - Original Paper
Rahmani, Zahra
Faridnia, Roghiyeh
Kalani, Hamed
Ghanei, Naryan
Fakhar, Mahdi
Zamanian, Marzieh
Keighobadi, Masoud
Tabaripour, Rabeeh
Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii
title Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii
title_full Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii
title_fullStr Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii
title_full_unstemmed Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii
title_short Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii
title_sort comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of leishmania major and toxoplasma gondii
topic Protozoology - Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829060/
https://www.ncbi.nlm.nih.gov/pubmed/33491113
http://dx.doi.org/10.1007/s00436-021-07058-2
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