Cargando…
Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii
This study aimed to evaluate the efficacy of amniotic fluid (AF) as an alternative to fetal bovine serum (FBS) in the maintenance of Leishmania major promastigotes and Toxoplasma gondii tachyzoites. AF was collected by an obstetrician using sterile syringes during a cesarean section. The culture med...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829060/ https://www.ncbi.nlm.nih.gov/pubmed/33491113 http://dx.doi.org/10.1007/s00436-021-07058-2 |
_version_ | 1783641110221946880 |
---|---|
author | Rahmani, Zahra Faridnia, Roghiyeh Kalani, Hamed Ghanei, Naryan Fakhar, Mahdi Zamanian, Marzieh Keighobadi, Masoud Tabaripour, Rabeeh |
author_facet | Rahmani, Zahra Faridnia, Roghiyeh Kalani, Hamed Ghanei, Naryan Fakhar, Mahdi Zamanian, Marzieh Keighobadi, Masoud Tabaripour, Rabeeh |
author_sort | Rahmani, Zahra |
collection | PubMed |
description | This study aimed to evaluate the efficacy of amniotic fluid (AF) as an alternative to fetal bovine serum (FBS) in the maintenance of Leishmania major promastigotes and Toxoplasma gondii tachyzoites. AF was collected by an obstetrician using sterile syringes during a cesarean section. The culture medium was supplemented with 5 different concentrations of FBS or AF including 2, 5, 10, 20, and 30%. These concentrations were used to maintain both mentioned parasites. L. major was maintained at temperatures 4 and 24 °C and examined once a week for 4 weeks, while T. gondii was maintained at temperatures 4, 24, and 37 °C and examined at hours 24, 48, 72, and 96. For L. major, at both 4 and 24 °C, we observed no significant difference between FBS and AF on day 7. However, on days 14, 21, and 28, the difference between FBS and AF was significant at both temperatures. For T. gondii, no significant difference was observed between FBS and AF at hour 24 and all temperatures. However, this difference was significant at hours 48, 72, and 96 and all temperatures. According to our results, although FBS had a greater efficacy than AF in the growth of L. major and the survival of T. gondii, the number of promastigotes increased over time in AF-containing medium and the number of tachyzoites reduced slowly with a mild slop. Therefore, AF can be a potential alternative to FBS. |
format | Online Article Text |
id | pubmed-7829060 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-78290602021-01-25 Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii Rahmani, Zahra Faridnia, Roghiyeh Kalani, Hamed Ghanei, Naryan Fakhar, Mahdi Zamanian, Marzieh Keighobadi, Masoud Tabaripour, Rabeeh Parasitol Res Protozoology - Original Paper This study aimed to evaluate the efficacy of amniotic fluid (AF) as an alternative to fetal bovine serum (FBS) in the maintenance of Leishmania major promastigotes and Toxoplasma gondii tachyzoites. AF was collected by an obstetrician using sterile syringes during a cesarean section. The culture medium was supplemented with 5 different concentrations of FBS or AF including 2, 5, 10, 20, and 30%. These concentrations were used to maintain both mentioned parasites. L. major was maintained at temperatures 4 and 24 °C and examined once a week for 4 weeks, while T. gondii was maintained at temperatures 4, 24, and 37 °C and examined at hours 24, 48, 72, and 96. For L. major, at both 4 and 24 °C, we observed no significant difference between FBS and AF on day 7. However, on days 14, 21, and 28, the difference between FBS and AF was significant at both temperatures. For T. gondii, no significant difference was observed between FBS and AF at hour 24 and all temperatures. However, this difference was significant at hours 48, 72, and 96 and all temperatures. According to our results, although FBS had a greater efficacy than AF in the growth of L. major and the survival of T. gondii, the number of promastigotes increased over time in AF-containing medium and the number of tachyzoites reduced slowly with a mild slop. Therefore, AF can be a potential alternative to FBS. Springer Berlin Heidelberg 2021-01-25 2021 /pmc/articles/PMC7829060/ /pubmed/33491113 http://dx.doi.org/10.1007/s00436-021-07058-2 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Protozoology - Original Paper Rahmani, Zahra Faridnia, Roghiyeh Kalani, Hamed Ghanei, Naryan Fakhar, Mahdi Zamanian, Marzieh Keighobadi, Masoud Tabaripour, Rabeeh Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii |
title | Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii |
title_full | Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii |
title_fullStr | Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii |
title_full_unstemmed | Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii |
title_short | Comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of Leishmania major and Toxoplasma gondii |
title_sort | comparative evaluation of amniotic fluid as an alternative to fetal bovine serum in the maintenance of leishmania major and toxoplasma gondii |
topic | Protozoology - Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829060/ https://www.ncbi.nlm.nih.gov/pubmed/33491113 http://dx.doi.org/10.1007/s00436-021-07058-2 |
work_keys_str_mv | AT rahmanizahra comparativeevaluationofamnioticfluidasanalternativetofetalbovineseruminthemaintenanceofleishmaniamajorandtoxoplasmagondii AT faridniaroghiyeh comparativeevaluationofamnioticfluidasanalternativetofetalbovineseruminthemaintenanceofleishmaniamajorandtoxoplasmagondii AT kalanihamed comparativeevaluationofamnioticfluidasanalternativetofetalbovineseruminthemaintenanceofleishmaniamajorandtoxoplasmagondii AT ghaneinaryan comparativeevaluationofamnioticfluidasanalternativetofetalbovineseruminthemaintenanceofleishmaniamajorandtoxoplasmagondii AT fakharmahdi comparativeevaluationofamnioticfluidasanalternativetofetalbovineseruminthemaintenanceofleishmaniamajorandtoxoplasmagondii AT zamanianmarzieh comparativeevaluationofamnioticfluidasanalternativetofetalbovineseruminthemaintenanceofleishmaniamajorandtoxoplasmagondii AT keighobadimasoud comparativeevaluationofamnioticfluidasanalternativetofetalbovineseruminthemaintenanceofleishmaniamajorandtoxoplasmagondii AT tabaripourrabeeh comparativeevaluationofamnioticfluidasanalternativetofetalbovineseruminthemaintenanceofleishmaniamajorandtoxoplasmagondii |