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Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon

INTRODUCTION: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and >15 hours af...

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Autores principales: Jan, Zala, Drab, Mitja, Drobne, Damjana, Bedina Zavec, Apolonija, Benčina, Mojca, Drasler, Barbara, Hočevar, Matej, Krek, Judita Lea, Pađen, Ljubiša, Pajnič, Manca, Repar, Neža, Šimunič, Boštjan, Štukelj, Roman, Kralj-Iglič, Veronika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829122/
https://www.ncbi.nlm.nih.gov/pubmed/33505159
http://dx.doi.org/10.2147/IJN.S282200
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author Jan, Zala
Drab, Mitja
Drobne, Damjana
Bedina Zavec, Apolonija
Benčina, Mojca
Drasler, Barbara
Hočevar, Matej
Krek, Judita Lea
Pađen, Ljubiša
Pajnič, Manca
Repar, Neža
Šimunič, Boštjan
Štukelj, Roman
Kralj-Iglič, Veronika
author_facet Jan, Zala
Drab, Mitja
Drobne, Damjana
Bedina Zavec, Apolonija
Benčina, Mojca
Drasler, Barbara
Hočevar, Matej
Krek, Judita Lea
Pađen, Ljubiša
Pajnič, Manca
Repar, Neža
Šimunič, Boštjan
Štukelj, Roman
Kralj-Iglič, Veronika
author_sort Jan, Zala
collection PubMed
description INTRODUCTION: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and >15 hours after physical effort. METHODS: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. RESULTS: More than 15 hours after physical effort a decrease was found in CNVs’ concentration in isolates from blood (46%; p<0.05), in ChE activity in whole blood (47%; p<0.001), in plasma (34%; p<0.01), and in erythrocyte suspension (54%; p<0.001), as well as in GST activity in erythrocyte suspension (16%; p<0.01) and in IL-6 concentration in plasma (63%; p<0.05). We found no change in GST activity in plasma and in TNF-α concentration in plasma. Correlations (>0.8; p<0.001) between CNVs’ concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. CONCLUSION: We found that >15 hours post-physical effort, CNVs’ concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs’ concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds.
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spelling pubmed-78291222021-01-26 Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon Jan, Zala Drab, Mitja Drobne, Damjana Bedina Zavec, Apolonija Benčina, Mojca Drasler, Barbara Hočevar, Matej Krek, Judita Lea Pađen, Ljubiša Pajnič, Manca Repar, Neža Šimunič, Boštjan Štukelj, Roman Kralj-Iglič, Veronika Int J Nanomedicine Original Research INTRODUCTION: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and >15 hours after physical effort. METHODS: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. RESULTS: More than 15 hours after physical effort a decrease was found in CNVs’ concentration in isolates from blood (46%; p<0.05), in ChE activity in whole blood (47%; p<0.001), in plasma (34%; p<0.01), and in erythrocyte suspension (54%; p<0.001), as well as in GST activity in erythrocyte suspension (16%; p<0.01) and in IL-6 concentration in plasma (63%; p<0.05). We found no change in GST activity in plasma and in TNF-α concentration in plasma. Correlations (>0.8; p<0.001) between CNVs’ concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. CONCLUSION: We found that >15 hours post-physical effort, CNVs’ concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs’ concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds. Dove 2021-01-20 /pmc/articles/PMC7829122/ /pubmed/33505159 http://dx.doi.org/10.2147/IJN.S282200 Text en © 2021 Jan et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Jan, Zala
Drab, Mitja
Drobne, Damjana
Bedina Zavec, Apolonija
Benčina, Mojca
Drasler, Barbara
Hočevar, Matej
Krek, Judita Lea
Pađen, Ljubiša
Pajnič, Manca
Repar, Neža
Šimunič, Boštjan
Štukelj, Roman
Kralj-Iglič, Veronika
Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon
title Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon
title_full Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon
title_fullStr Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon
title_full_unstemmed Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon
title_short Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon
title_sort decrease in cellular nanovesicles concentration in blood of athletes more than 15 hours after marathon
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829122/
https://www.ncbi.nlm.nih.gov/pubmed/33505159
http://dx.doi.org/10.2147/IJN.S282200
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