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Plasticity of the Cuticular Transpiration Barrier in Response to Water Shortage and Resupply in Camellia sinensis: A Role of Cuticular Waxes

The cuticle is regarded as a non-living tissue; it remains unknown whether the cuticle could be reversibly modified and what are the potential mechanisms. In this study, three tea germplasms (Wuniuzao, 0202-10, and 0306A) were subjected to water deprivation followed by rehydration. The epicuticular...

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Autores principales: Zhang, Yi, Du, Zhenghua, Han, Yanting, Chen, Xiaobing, Kong, Xiangrui, Sun, Weijiang, Chen, Changsong, Chen, Mingjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829210/
https://www.ncbi.nlm.nih.gov/pubmed/33505410
http://dx.doi.org/10.3389/fpls.2020.600069
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author Zhang, Yi
Du, Zhenghua
Han, Yanting
Chen, Xiaobing
Kong, Xiangrui
Sun, Weijiang
Chen, Changsong
Chen, Mingjie
author_facet Zhang, Yi
Du, Zhenghua
Han, Yanting
Chen, Xiaobing
Kong, Xiangrui
Sun, Weijiang
Chen, Changsong
Chen, Mingjie
author_sort Zhang, Yi
collection PubMed
description The cuticle is regarded as a non-living tissue; it remains unknown whether the cuticle could be reversibly modified and what are the potential mechanisms. In this study, three tea germplasms (Wuniuzao, 0202-10, and 0306A) were subjected to water deprivation followed by rehydration. The epicuticular waxes and intracuticular waxes from both leaf surfaces were quantified from the mature 5th leaf. Cuticular transpiration rates were then measured from leaf drying curves, and the correlations between cuticular transpiration rates and cuticular wax coverage were analyzed. We found that the cuticular transpiration barriers were reinforced by drought and reversed by rehydration treatment; the initial weak cuticular transpiration barriers were preferentially reinforced by drought stress, while the original major cuticular transpiration barriers were either strengthened or unaltered. Correlation analysis suggests that cuticle modifications could be realized by selective deposition of specific wax compounds into individual cuticular compartments through multiple mechanisms, including in vivo wax synthesis or transport, dynamic phase separation between epicuticular waxes and the intracuticular waxes, in vitro polymerization, and retro transportation into epidermal cell wall or protoplast for further transformation. Our data suggest that modifications of a limited set of specific wax components from individual cuticular compartments are sufficient to alter cuticular transpiration barrier properties.
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spelling pubmed-78292102021-01-26 Plasticity of the Cuticular Transpiration Barrier in Response to Water Shortage and Resupply in Camellia sinensis: A Role of Cuticular Waxes Zhang, Yi Du, Zhenghua Han, Yanting Chen, Xiaobing Kong, Xiangrui Sun, Weijiang Chen, Changsong Chen, Mingjie Front Plant Sci Plant Science The cuticle is regarded as a non-living tissue; it remains unknown whether the cuticle could be reversibly modified and what are the potential mechanisms. In this study, three tea germplasms (Wuniuzao, 0202-10, and 0306A) were subjected to water deprivation followed by rehydration. The epicuticular waxes and intracuticular waxes from both leaf surfaces were quantified from the mature 5th leaf. Cuticular transpiration rates were then measured from leaf drying curves, and the correlations between cuticular transpiration rates and cuticular wax coverage were analyzed. We found that the cuticular transpiration barriers were reinforced by drought and reversed by rehydration treatment; the initial weak cuticular transpiration barriers were preferentially reinforced by drought stress, while the original major cuticular transpiration barriers were either strengthened or unaltered. Correlation analysis suggests that cuticle modifications could be realized by selective deposition of specific wax compounds into individual cuticular compartments through multiple mechanisms, including in vivo wax synthesis or transport, dynamic phase separation between epicuticular waxes and the intracuticular waxes, in vitro polymerization, and retro transportation into epidermal cell wall or protoplast for further transformation. Our data suggest that modifications of a limited set of specific wax components from individual cuticular compartments are sufficient to alter cuticular transpiration barrier properties. Frontiers Media S.A. 2021-01-11 /pmc/articles/PMC7829210/ /pubmed/33505410 http://dx.doi.org/10.3389/fpls.2020.600069 Text en Copyright © 2021 Zhang, Du, Han, Chen, Kong, Sun, Chen and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhang, Yi
Du, Zhenghua
Han, Yanting
Chen, Xiaobing
Kong, Xiangrui
Sun, Weijiang
Chen, Changsong
Chen, Mingjie
Plasticity of the Cuticular Transpiration Barrier in Response to Water Shortage and Resupply in Camellia sinensis: A Role of Cuticular Waxes
title Plasticity of the Cuticular Transpiration Barrier in Response to Water Shortage and Resupply in Camellia sinensis: A Role of Cuticular Waxes
title_full Plasticity of the Cuticular Transpiration Barrier in Response to Water Shortage and Resupply in Camellia sinensis: A Role of Cuticular Waxes
title_fullStr Plasticity of the Cuticular Transpiration Barrier in Response to Water Shortage and Resupply in Camellia sinensis: A Role of Cuticular Waxes
title_full_unstemmed Plasticity of the Cuticular Transpiration Barrier in Response to Water Shortage and Resupply in Camellia sinensis: A Role of Cuticular Waxes
title_short Plasticity of the Cuticular Transpiration Barrier in Response to Water Shortage and Resupply in Camellia sinensis: A Role of Cuticular Waxes
title_sort plasticity of the cuticular transpiration barrier in response to water shortage and resupply in camellia sinensis: a role of cuticular waxes
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829210/
https://www.ncbi.nlm.nih.gov/pubmed/33505410
http://dx.doi.org/10.3389/fpls.2020.600069
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