Isolation and characterization of extracellular vesicles produced by cell lines

Cells produce two broad classes of extracellular vesicles (EVs), exosomes and microvesicles (MVs). Exosomes are 30–150 nm vesicles derived from multivesicular bodies, while MVs are 200–1,000 nm vesicles that pinch off from plasma membranes. Reliable isolation of EVs is crucial to understand their bi...

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Detalles Bibliográficos
Autores principales: Wang, Fangyu, Cerione, Richard A., Antonyak, Marc A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829267/
https://www.ncbi.nlm.nih.gov/pubmed/33532740
http://dx.doi.org/10.1016/j.xpro.2021.100295
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author Wang, Fangyu
Cerione, Richard A.
Antonyak, Marc A.
author_facet Wang, Fangyu
Cerione, Richard A.
Antonyak, Marc A.
author_sort Wang, Fangyu
collection PubMed
description Cells produce two broad classes of extracellular vesicles (EVs), exosomes and microvesicles (MVs). Exosomes are 30–150 nm vesicles derived from multivesicular bodies, while MVs are 200–1,000 nm vesicles that pinch off from plasma membranes. Reliable isolation of EVs is crucial to understand their biochemical and functional properties. Here, we describe a protocol to isolate and characterize EVs from conditioned medium from mammalian cell lines. This protocol has been optimized for adherent cells but can also be adapted for suspension cells. For complete details on the use and execution of this protocol, please refer to Latifkar et al. (2019).
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spelling pubmed-78292672021-02-01 Isolation and characterization of extracellular vesicles produced by cell lines Wang, Fangyu Cerione, Richard A. Antonyak, Marc A. STAR Protoc Protocol Cells produce two broad classes of extracellular vesicles (EVs), exosomes and microvesicles (MVs). Exosomes are 30–150 nm vesicles derived from multivesicular bodies, while MVs are 200–1,000 nm vesicles that pinch off from plasma membranes. Reliable isolation of EVs is crucial to understand their biochemical and functional properties. Here, we describe a protocol to isolate and characterize EVs from conditioned medium from mammalian cell lines. This protocol has been optimized for adherent cells but can also be adapted for suspension cells. For complete details on the use and execution of this protocol, please refer to Latifkar et al. (2019). Elsevier 2021-01-22 /pmc/articles/PMC7829267/ /pubmed/33532740 http://dx.doi.org/10.1016/j.xpro.2021.100295 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Wang, Fangyu
Cerione, Richard A.
Antonyak, Marc A.
Isolation and characterization of extracellular vesicles produced by cell lines
title Isolation and characterization of extracellular vesicles produced by cell lines
title_full Isolation and characterization of extracellular vesicles produced by cell lines
title_fullStr Isolation and characterization of extracellular vesicles produced by cell lines
title_full_unstemmed Isolation and characterization of extracellular vesicles produced by cell lines
title_short Isolation and characterization of extracellular vesicles produced by cell lines
title_sort isolation and characterization of extracellular vesicles produced by cell lines
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829267/
https://www.ncbi.nlm.nih.gov/pubmed/33532740
http://dx.doi.org/10.1016/j.xpro.2021.100295
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