Open-source computational framework for studying Drosophila behavioral phase

This protocol describes a standardized method for analyzing Drosophila behavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophila genetic crosses to behavioral experiments. We...

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Detalles Bibliográficos
Autores principales: Kostadinov, Boyan, Lee Pettibone, Hannah, Bell, Evardra Valerie, Zhou, Xiaona, Pranevicius, Ausra, Shafer, Orie Thomas, Fernandez, Maria Paz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829270/
https://www.ncbi.nlm.nih.gov/pubmed/33532734
http://dx.doi.org/10.1016/j.xpro.2020.100285
Descripción
Sumario:This protocol describes a standardized method for analyzing Drosophila behavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophila genetic crosses to behavioral experiments. We also provide an open-source computational framework using R for the analysis of the phase of behavior using circular statistics. An extended method for complete use is also provided. For complete details on the use and execution of this protocol, please refer to Fernandez et al. (2020).

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