Open-source computational framework for studying Drosophila behavioral phase

This protocol describes a standardized method for analyzing Drosophila behavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophila genetic crosses to behavioral experiments. We...

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Detalles Bibliográficos
Autores principales: Kostadinov, Boyan, Lee Pettibone, Hannah, Bell, Evardra Valerie, Zhou, Xiaona, Pranevicius, Ausra, Shafer, Orie Thomas, Fernandez, Maria Paz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829270/
https://www.ncbi.nlm.nih.gov/pubmed/33532734
http://dx.doi.org/10.1016/j.xpro.2020.100285
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author Kostadinov, Boyan
Lee Pettibone, Hannah
Bell, Evardra Valerie
Zhou, Xiaona
Pranevicius, Ausra
Shafer, Orie Thomas
Fernandez, Maria Paz
author_facet Kostadinov, Boyan
Lee Pettibone, Hannah
Bell, Evardra Valerie
Zhou, Xiaona
Pranevicius, Ausra
Shafer, Orie Thomas
Fernandez, Maria Paz
author_sort Kostadinov, Boyan
collection PubMed
description This protocol describes a standardized method for analyzing Drosophila behavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophila genetic crosses to behavioral experiments. We also provide an open-source computational framework using R for the analysis of the phase of behavior using circular statistics. An extended method for complete use is also provided. For complete details on the use and execution of this protocol, please refer to Fernandez et al. (2020).
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spelling pubmed-78292702021-02-01 Open-source computational framework for studying Drosophila behavioral phase Kostadinov, Boyan Lee Pettibone, Hannah Bell, Evardra Valerie Zhou, Xiaona Pranevicius, Ausra Shafer, Orie Thomas Fernandez, Maria Paz STAR Protoc Protocol This protocol describes a standardized method for analyzing Drosophila behavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophila genetic crosses to behavioral experiments. We also provide an open-source computational framework using R for the analysis of the phase of behavior using circular statistics. An extended method for complete use is also provided. For complete details on the use and execution of this protocol, please refer to Fernandez et al. (2020). Elsevier 2021-01-22 /pmc/articles/PMC7829270/ /pubmed/33532734 http://dx.doi.org/10.1016/j.xpro.2020.100285 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kostadinov, Boyan
Lee Pettibone, Hannah
Bell, Evardra Valerie
Zhou, Xiaona
Pranevicius, Ausra
Shafer, Orie Thomas
Fernandez, Maria Paz
Open-source computational framework for studying Drosophila behavioral phase
title Open-source computational framework for studying Drosophila behavioral phase
title_full Open-source computational framework for studying Drosophila behavioral phase
title_fullStr Open-source computational framework for studying Drosophila behavioral phase
title_full_unstemmed Open-source computational framework for studying Drosophila behavioral phase
title_short Open-source computational framework for studying Drosophila behavioral phase
title_sort open-source computational framework for studying drosophila behavioral phase
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829270/
https://www.ncbi.nlm.nih.gov/pubmed/33532734
http://dx.doi.org/10.1016/j.xpro.2020.100285
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