Measuring microenvironment-tuned nuclear stiffness of cancer cells with atomic force microscopy

Quantification of nuclear stiffness is challenging for cells encapsulated within a 3D extracellular matrix (ECM). Here, we describe an experimental setup for measuring microenvironment-dependent tuning of nuclear stiffness using an atomic force microscope (AFM). In our setup, ECM-coated polyacrylami...

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Detalles Bibliográficos
Autores principales: Barai, Amlan, Das, Alakesh, Sen, Shamik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829340/
https://www.ncbi.nlm.nih.gov/pubmed/33532741
http://dx.doi.org/10.1016/j.xpro.2021.100296

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