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Changes of Metabolites in Acute Ischemic Stroke and Its Subtypes

Existing techniques have many limitations in the diagnosis and classification of ischemic stroke (IS). Considering this, we used metabolomics to screen for potential biomarkers of IS and its subtypes and to explore the underlying related pathophysiological mechanisms. Serum samples from 99 patients...

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Autores principales: Wang, Xin, Zhang, Luyang, Sun, Wenxian, Pei, Lu-lu, Tian, Mengke, Liang, Jing, Liu, Xinjing, Zhang, Rui, Fang, Hui, Wu, Jun, Sun, Shilei, Xu, Yuming, Kang, Jian-Sheng, Song, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829509/
https://www.ncbi.nlm.nih.gov/pubmed/33505234
http://dx.doi.org/10.3389/fnins.2020.580929
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author Wang, Xin
Zhang, Luyang
Sun, Wenxian
Pei, Lu-lu
Tian, Mengke
Liang, Jing
Liu, Xinjing
Zhang, Rui
Fang, Hui
Wu, Jun
Sun, Shilei
Xu, Yuming
Kang, Jian-Sheng
Song, Bo
author_facet Wang, Xin
Zhang, Luyang
Sun, Wenxian
Pei, Lu-lu
Tian, Mengke
Liang, Jing
Liu, Xinjing
Zhang, Rui
Fang, Hui
Wu, Jun
Sun, Shilei
Xu, Yuming
Kang, Jian-Sheng
Song, Bo
author_sort Wang, Xin
collection PubMed
description Existing techniques have many limitations in the diagnosis and classification of ischemic stroke (IS). Considering this, we used metabolomics to screen for potential biomarkers of IS and its subtypes and to explore the underlying related pathophysiological mechanisms. Serum samples from 99 patients with acute ischemic stroke (AIS) [the AIS subtypes included 49 patients with large artery atherosclerosis (LAA) and 50 patients with small artery occlusion (SAO)] and 50 matched healthy controls (HCs) were analyzed by non-targeted metabolomics based on liquid chromatography–mass spectrometry. A multivariate statistical analysis was performed to identify potential biomarkers. There were 18 significantly different metabolites, such as oleic acid, linoleic acid, arachidonic acid, L-glutamine, L-arginine, and L-proline, between patients with AIS and HCs. These different metabolites are closely related to many metabolic pathways, such as fatty acid metabolism and amino acid metabolism. There were also differences in metabolic profiling between the LAA and SAO groups. There were eight different metabolites, including L-pipecolic acid, 1-Methylhistidine, PE, LysoPE, and LysoPC, which affected glycerophospholipid metabolism, glycosylphosphatidylinositol-anchor biosynthesis, histidine metabolism, and lysine degradation. Our study effectively identified the metabolic profiles of IS and its subtypes. The different metabolites between LAA and SAO may be potential biomarkers in the context of clinical diagnosis. These results highlight the potential of metabolomics to reveal new pathways for IS subtypes and provide a new avenue to explore the pathophysiological mechanisms underlying IS and its subtypes.
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spelling pubmed-78295092021-01-26 Changes of Metabolites in Acute Ischemic Stroke and Its Subtypes Wang, Xin Zhang, Luyang Sun, Wenxian Pei, Lu-lu Tian, Mengke Liang, Jing Liu, Xinjing Zhang, Rui Fang, Hui Wu, Jun Sun, Shilei Xu, Yuming Kang, Jian-Sheng Song, Bo Front Neurosci Neuroscience Existing techniques have many limitations in the diagnosis and classification of ischemic stroke (IS). Considering this, we used metabolomics to screen for potential biomarkers of IS and its subtypes and to explore the underlying related pathophysiological mechanisms. Serum samples from 99 patients with acute ischemic stroke (AIS) [the AIS subtypes included 49 patients with large artery atherosclerosis (LAA) and 50 patients with small artery occlusion (SAO)] and 50 matched healthy controls (HCs) were analyzed by non-targeted metabolomics based on liquid chromatography–mass spectrometry. A multivariate statistical analysis was performed to identify potential biomarkers. There were 18 significantly different metabolites, such as oleic acid, linoleic acid, arachidonic acid, L-glutamine, L-arginine, and L-proline, between patients with AIS and HCs. These different metabolites are closely related to many metabolic pathways, such as fatty acid metabolism and amino acid metabolism. There were also differences in metabolic profiling between the LAA and SAO groups. There were eight different metabolites, including L-pipecolic acid, 1-Methylhistidine, PE, LysoPE, and LysoPC, which affected glycerophospholipid metabolism, glycosylphosphatidylinositol-anchor biosynthesis, histidine metabolism, and lysine degradation. Our study effectively identified the metabolic profiles of IS and its subtypes. The different metabolites between LAA and SAO may be potential biomarkers in the context of clinical diagnosis. These results highlight the potential of metabolomics to reveal new pathways for IS subtypes and provide a new avenue to explore the pathophysiological mechanisms underlying IS and its subtypes. Frontiers Media S.A. 2021-01-11 /pmc/articles/PMC7829509/ /pubmed/33505234 http://dx.doi.org/10.3389/fnins.2020.580929 Text en Copyright © 2021 Wang, Zhang, Sun, Pei, Tian, Liang, Liu, Zhang, Fang, Wu, Sun, Xu, Kang and Song. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Wang, Xin
Zhang, Luyang
Sun, Wenxian
Pei, Lu-lu
Tian, Mengke
Liang, Jing
Liu, Xinjing
Zhang, Rui
Fang, Hui
Wu, Jun
Sun, Shilei
Xu, Yuming
Kang, Jian-Sheng
Song, Bo
Changes of Metabolites in Acute Ischemic Stroke and Its Subtypes
title Changes of Metabolites in Acute Ischemic Stroke and Its Subtypes
title_full Changes of Metabolites in Acute Ischemic Stroke and Its Subtypes
title_fullStr Changes of Metabolites in Acute Ischemic Stroke and Its Subtypes
title_full_unstemmed Changes of Metabolites in Acute Ischemic Stroke and Its Subtypes
title_short Changes of Metabolites in Acute Ischemic Stroke and Its Subtypes
title_sort changes of metabolites in acute ischemic stroke and its subtypes
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829509/
https://www.ncbi.nlm.nih.gov/pubmed/33505234
http://dx.doi.org/10.3389/fnins.2020.580929
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