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Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine
Mechanistic understanding of germ cell formation at a genome-scale level can aid in developing novel therapeutic strategies for infertility. Germ cell formation is a complex process that is regulated by various mechanisms, including epigenetic regulation, germ cell-specific gene transcription, and m...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829788/ https://www.ncbi.nlm.nih.gov/pubmed/33467661 http://dx.doi.org/10.3390/ijms22020823 |
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author | La, Hyeonwoo Yoo, Hyunjin Lee, Eun Joo Thang, Nguyen Xuan Choi, Hee Jin Oh, Jeongheon Park, Ji Hyun Hong, Kwonho |
author_facet | La, Hyeonwoo Yoo, Hyunjin Lee, Eun Joo Thang, Nguyen Xuan Choi, Hee Jin Oh, Jeongheon Park, Ji Hyun Hong, Kwonho |
author_sort | La, Hyeonwoo |
collection | PubMed |
description | Mechanistic understanding of germ cell formation at a genome-scale level can aid in developing novel therapeutic strategies for infertility. Germ cell formation is a complex process that is regulated by various mechanisms, including epigenetic regulation, germ cell-specific gene transcription, and meiosis. Gonads contain a limited number of germ cells at various stages of differentiation. Hence, genome-scale analysis of germ cells at the single-cell level is challenging. Conventional genome-scale approaches cannot delineate the landscape of genomic, transcriptomic, and epigenomic diversity or heterogeneity in the differentiating germ cells of gonads. Recent advances in single-cell genomic techniques along with single-cell isolation methods, such as microfluidics and fluorescence-activated cell sorting, have helped elucidate the mechanisms underlying germ cell development and reproductive disorders in humans. In this review, the history of single-cell transcriptomic analysis and their technical advantages over the conventional methods have been discussed. Additionally, recent applications of single-cell transcriptomic analysis for analyzing germ cells have been summarized. |
format | Online Article Text |
id | pubmed-7829788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78297882021-01-26 Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine La, Hyeonwoo Yoo, Hyunjin Lee, Eun Joo Thang, Nguyen Xuan Choi, Hee Jin Oh, Jeongheon Park, Ji Hyun Hong, Kwonho Int J Mol Sci Review Mechanistic understanding of germ cell formation at a genome-scale level can aid in developing novel therapeutic strategies for infertility. Germ cell formation is a complex process that is regulated by various mechanisms, including epigenetic regulation, germ cell-specific gene transcription, and meiosis. Gonads contain a limited number of germ cells at various stages of differentiation. Hence, genome-scale analysis of germ cells at the single-cell level is challenging. Conventional genome-scale approaches cannot delineate the landscape of genomic, transcriptomic, and epigenomic diversity or heterogeneity in the differentiating germ cells of gonads. Recent advances in single-cell genomic techniques along with single-cell isolation methods, such as microfluidics and fluorescence-activated cell sorting, have helped elucidate the mechanisms underlying germ cell development and reproductive disorders in humans. In this review, the history of single-cell transcriptomic analysis and their technical advantages over the conventional methods have been discussed. Additionally, recent applications of single-cell transcriptomic analysis for analyzing germ cells have been summarized. MDPI 2021-01-15 /pmc/articles/PMC7829788/ /pubmed/33467661 http://dx.doi.org/10.3390/ijms22020823 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review La, Hyeonwoo Yoo, Hyunjin Lee, Eun Joo Thang, Nguyen Xuan Choi, Hee Jin Oh, Jeongheon Park, Ji Hyun Hong, Kwonho Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine |
title | Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine |
title_full | Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine |
title_fullStr | Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine |
title_full_unstemmed | Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine |
title_short | Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine |
title_sort | insights from the applications of single-cell transcriptomic analysis in germ cell development and reproductive medicine |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829788/ https://www.ncbi.nlm.nih.gov/pubmed/33467661 http://dx.doi.org/10.3390/ijms22020823 |
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