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Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine

Mechanistic understanding of germ cell formation at a genome-scale level can aid in developing novel therapeutic strategies for infertility. Germ cell formation is a complex process that is regulated by various mechanisms, including epigenetic regulation, germ cell-specific gene transcription, and m...

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Autores principales: La, Hyeonwoo, Yoo, Hyunjin, Lee, Eun Joo, Thang, Nguyen Xuan, Choi, Hee Jin, Oh, Jeongheon, Park, Ji Hyun, Hong, Kwonho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829788/
https://www.ncbi.nlm.nih.gov/pubmed/33467661
http://dx.doi.org/10.3390/ijms22020823
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author La, Hyeonwoo
Yoo, Hyunjin
Lee, Eun Joo
Thang, Nguyen Xuan
Choi, Hee Jin
Oh, Jeongheon
Park, Ji Hyun
Hong, Kwonho
author_facet La, Hyeonwoo
Yoo, Hyunjin
Lee, Eun Joo
Thang, Nguyen Xuan
Choi, Hee Jin
Oh, Jeongheon
Park, Ji Hyun
Hong, Kwonho
author_sort La, Hyeonwoo
collection PubMed
description Mechanistic understanding of germ cell formation at a genome-scale level can aid in developing novel therapeutic strategies for infertility. Germ cell formation is a complex process that is regulated by various mechanisms, including epigenetic regulation, germ cell-specific gene transcription, and meiosis. Gonads contain a limited number of germ cells at various stages of differentiation. Hence, genome-scale analysis of germ cells at the single-cell level is challenging. Conventional genome-scale approaches cannot delineate the landscape of genomic, transcriptomic, and epigenomic diversity or heterogeneity in the differentiating germ cells of gonads. Recent advances in single-cell genomic techniques along with single-cell isolation methods, such as microfluidics and fluorescence-activated cell sorting, have helped elucidate the mechanisms underlying germ cell development and reproductive disorders in humans. In this review, the history of single-cell transcriptomic analysis and their technical advantages over the conventional methods have been discussed. Additionally, recent applications of single-cell transcriptomic analysis for analyzing germ cells have been summarized.
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spelling pubmed-78297882021-01-26 Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine La, Hyeonwoo Yoo, Hyunjin Lee, Eun Joo Thang, Nguyen Xuan Choi, Hee Jin Oh, Jeongheon Park, Ji Hyun Hong, Kwonho Int J Mol Sci Review Mechanistic understanding of germ cell formation at a genome-scale level can aid in developing novel therapeutic strategies for infertility. Germ cell formation is a complex process that is regulated by various mechanisms, including epigenetic regulation, germ cell-specific gene transcription, and meiosis. Gonads contain a limited number of germ cells at various stages of differentiation. Hence, genome-scale analysis of germ cells at the single-cell level is challenging. Conventional genome-scale approaches cannot delineate the landscape of genomic, transcriptomic, and epigenomic diversity or heterogeneity in the differentiating germ cells of gonads. Recent advances in single-cell genomic techniques along with single-cell isolation methods, such as microfluidics and fluorescence-activated cell sorting, have helped elucidate the mechanisms underlying germ cell development and reproductive disorders in humans. In this review, the history of single-cell transcriptomic analysis and their technical advantages over the conventional methods have been discussed. Additionally, recent applications of single-cell transcriptomic analysis for analyzing germ cells have been summarized. MDPI 2021-01-15 /pmc/articles/PMC7829788/ /pubmed/33467661 http://dx.doi.org/10.3390/ijms22020823 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
La, Hyeonwoo
Yoo, Hyunjin
Lee, Eun Joo
Thang, Nguyen Xuan
Choi, Hee Jin
Oh, Jeongheon
Park, Ji Hyun
Hong, Kwonho
Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine
title Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine
title_full Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine
title_fullStr Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine
title_full_unstemmed Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine
title_short Insights from the Applications of Single-Cell Transcriptomic Analysis in Germ Cell Development and Reproductive Medicine
title_sort insights from the applications of single-cell transcriptomic analysis in germ cell development and reproductive medicine
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829788/
https://www.ncbi.nlm.nih.gov/pubmed/33467661
http://dx.doi.org/10.3390/ijms22020823
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