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Non-Invasive Method to Detect Infection with Mycobacterium tuberculosis Complex in Wild Boar by Measurement of Volatile Organic Compounds Obtained from Feces with an Electronic Nose System

More effective methods to detect bovine tuberculosis, caused by Mycobacterium bovis, in wildlife, is of paramount importance for preventing disease spread to other wild animals, livestock, and human beings. In this study, we analyzed the volatile organic compounds emitted by fecal samples collected...

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Autores principales: de Jesús Beleño-Sáenz, Kelvin, Cáceres-Tarazona, Juan Martín, Nol, Pauline, Jaimes-Mogollón, Aylen Lisset, Gualdrón-Guerrero, Oscar Eduardo, Durán-Acevedo, Cristhian Manuel, Barasona, Jose Angel, Vicente, Joaquin, Torres, María José, Welearegay, Tesfalem Geremariam, Österlund, Lars, Rhyan, Jack, Ionescu, Radu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829825/
https://www.ncbi.nlm.nih.gov/pubmed/33467480
http://dx.doi.org/10.3390/s21020584
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author de Jesús Beleño-Sáenz, Kelvin
Cáceres-Tarazona, Juan Martín
Nol, Pauline
Jaimes-Mogollón, Aylen Lisset
Gualdrón-Guerrero, Oscar Eduardo
Durán-Acevedo, Cristhian Manuel
Barasona, Jose Angel
Vicente, Joaquin
Torres, María José
Welearegay, Tesfalem Geremariam
Österlund, Lars
Rhyan, Jack
Ionescu, Radu
author_facet de Jesús Beleño-Sáenz, Kelvin
Cáceres-Tarazona, Juan Martín
Nol, Pauline
Jaimes-Mogollón, Aylen Lisset
Gualdrón-Guerrero, Oscar Eduardo
Durán-Acevedo, Cristhian Manuel
Barasona, Jose Angel
Vicente, Joaquin
Torres, María José
Welearegay, Tesfalem Geremariam
Österlund, Lars
Rhyan, Jack
Ionescu, Radu
author_sort de Jesús Beleño-Sáenz, Kelvin
collection PubMed
description More effective methods to detect bovine tuberculosis, caused by Mycobacterium bovis, in wildlife, is of paramount importance for preventing disease spread to other wild animals, livestock, and human beings. In this study, we analyzed the volatile organic compounds emitted by fecal samples collected from free-ranging wild boar captured in Doñana National Park, Spain, with an electronic nose system based on organically-functionalized gold nanoparticles. The animals were separated by the age group for performing the analysis. Adult (>24 months) and sub-adult (12–24 months) animals were anesthetized before sample collection, whereas the juvenile (<12 months) animals were manually restrained while collecting the sample. Good accuracy was obtained for the adult and sub-adult classification models: 100% during the training phase and 88.9% during the testing phase for the adult animals, and 100% during both the training and testing phase for the sub-adult animals, respectively. The results obtained could be important for the further development of a non-invasive and less expensive detection method of bovine tuberculosis in wildlife populations.
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spelling pubmed-78298252021-01-26 Non-Invasive Method to Detect Infection with Mycobacterium tuberculosis Complex in Wild Boar by Measurement of Volatile Organic Compounds Obtained from Feces with an Electronic Nose System de Jesús Beleño-Sáenz, Kelvin Cáceres-Tarazona, Juan Martín Nol, Pauline Jaimes-Mogollón, Aylen Lisset Gualdrón-Guerrero, Oscar Eduardo Durán-Acevedo, Cristhian Manuel Barasona, Jose Angel Vicente, Joaquin Torres, María José Welearegay, Tesfalem Geremariam Österlund, Lars Rhyan, Jack Ionescu, Radu Sensors (Basel) Article More effective methods to detect bovine tuberculosis, caused by Mycobacterium bovis, in wildlife, is of paramount importance for preventing disease spread to other wild animals, livestock, and human beings. In this study, we analyzed the volatile organic compounds emitted by fecal samples collected from free-ranging wild boar captured in Doñana National Park, Spain, with an electronic nose system based on organically-functionalized gold nanoparticles. The animals were separated by the age group for performing the analysis. Adult (>24 months) and sub-adult (12–24 months) animals were anesthetized before sample collection, whereas the juvenile (<12 months) animals were manually restrained while collecting the sample. Good accuracy was obtained for the adult and sub-adult classification models: 100% during the training phase and 88.9% during the testing phase for the adult animals, and 100% during both the training and testing phase for the sub-adult animals, respectively. The results obtained could be important for the further development of a non-invasive and less expensive detection method of bovine tuberculosis in wildlife populations. MDPI 2021-01-15 /pmc/articles/PMC7829825/ /pubmed/33467480 http://dx.doi.org/10.3390/s21020584 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Jesús Beleño-Sáenz, Kelvin
Cáceres-Tarazona, Juan Martín
Nol, Pauline
Jaimes-Mogollón, Aylen Lisset
Gualdrón-Guerrero, Oscar Eduardo
Durán-Acevedo, Cristhian Manuel
Barasona, Jose Angel
Vicente, Joaquin
Torres, María José
Welearegay, Tesfalem Geremariam
Österlund, Lars
Rhyan, Jack
Ionescu, Radu
Non-Invasive Method to Detect Infection with Mycobacterium tuberculosis Complex in Wild Boar by Measurement of Volatile Organic Compounds Obtained from Feces with an Electronic Nose System
title Non-Invasive Method to Detect Infection with Mycobacterium tuberculosis Complex in Wild Boar by Measurement of Volatile Organic Compounds Obtained from Feces with an Electronic Nose System
title_full Non-Invasive Method to Detect Infection with Mycobacterium tuberculosis Complex in Wild Boar by Measurement of Volatile Organic Compounds Obtained from Feces with an Electronic Nose System
title_fullStr Non-Invasive Method to Detect Infection with Mycobacterium tuberculosis Complex in Wild Boar by Measurement of Volatile Organic Compounds Obtained from Feces with an Electronic Nose System
title_full_unstemmed Non-Invasive Method to Detect Infection with Mycobacterium tuberculosis Complex in Wild Boar by Measurement of Volatile Organic Compounds Obtained from Feces with an Electronic Nose System
title_short Non-Invasive Method to Detect Infection with Mycobacterium tuberculosis Complex in Wild Boar by Measurement of Volatile Organic Compounds Obtained from Feces with an Electronic Nose System
title_sort non-invasive method to detect infection with mycobacterium tuberculosis complex in wild boar by measurement of volatile organic compounds obtained from feces with an electronic nose system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829825/
https://www.ncbi.nlm.nih.gov/pubmed/33467480
http://dx.doi.org/10.3390/s21020584
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