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Correlating Local Volumetric Tissue Strains with Global Lung Mechanics Measurements

The mechanics of breathing is a fascinating and vital process. The lung has complexities and subtle heterogeneities in structure across length scales that influence mechanics and function. This study establishes an experimental pipeline for capturing alveolar deformations during a respiratory cycle...

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Detalles Bibliográficos
Autores principales: Arora, Hari, Mitchell, Ria L., Johnston, Richard, Manolesos, Marinos, Howells, David, Sherwood, Joseph M., Bodey, Andrew J., Wanelik, Kaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829924/
https://www.ncbi.nlm.nih.gov/pubmed/33477444
http://dx.doi.org/10.3390/ma14020439
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author Arora, Hari
Mitchell, Ria L.
Johnston, Richard
Manolesos, Marinos
Howells, David
Sherwood, Joseph M.
Bodey, Andrew J.
Wanelik, Kaz
author_facet Arora, Hari
Mitchell, Ria L.
Johnston, Richard
Manolesos, Marinos
Howells, David
Sherwood, Joseph M.
Bodey, Andrew J.
Wanelik, Kaz
author_sort Arora, Hari
collection PubMed
description The mechanics of breathing is a fascinating and vital process. The lung has complexities and subtle heterogeneities in structure across length scales that influence mechanics and function. This study establishes an experimental pipeline for capturing alveolar deformations during a respiratory cycle using synchrotron radiation micro-computed tomography (SR-micro-CT). Rodent lungs were mechanically ventilated and imaged at various time points during the respiratory cycle. Pressure-Volume (P-V) characteristics were recorded to capture any changes in overall lung mechanical behaviour during the experiment. A sequence of tomograms was collected from the lungs within the intact thoracic cavity. Digital volume correlation (DVC) was used to compute the three-dimensional strain field at the alveolar level from the time sequence of reconstructed tomograms. Regional differences in ventilation were highlighted during the respiratory cycle, relating the local strains within the lung tissue to the global ventilation measurements. Strains locally reached approximately 150% compared to the averaged regional deformations of approximately 80–100%. Redistribution of air within the lungs was observed during cycling. Regions which were relatively poorly ventilated (low deformations compared to its neighbouring region) were deforming more uniformly at later stages of the experiment (consistent with its neighbouring region). Such heterogenous phenomena are common in everyday breathing. In pathological lungs, some of these non-uniformities in deformation behaviour can become exaggerated, leading to poor function or further damage. The technique presented can help characterize the multiscale biomechanical nature of a given pathology to improve patient management strategies, considering both the local and global lung mechanics.
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spelling pubmed-78299242021-01-26 Correlating Local Volumetric Tissue Strains with Global Lung Mechanics Measurements Arora, Hari Mitchell, Ria L. Johnston, Richard Manolesos, Marinos Howells, David Sherwood, Joseph M. Bodey, Andrew J. Wanelik, Kaz Materials (Basel) Article The mechanics of breathing is a fascinating and vital process. The lung has complexities and subtle heterogeneities in structure across length scales that influence mechanics and function. This study establishes an experimental pipeline for capturing alveolar deformations during a respiratory cycle using synchrotron radiation micro-computed tomography (SR-micro-CT). Rodent lungs were mechanically ventilated and imaged at various time points during the respiratory cycle. Pressure-Volume (P-V) characteristics were recorded to capture any changes in overall lung mechanical behaviour during the experiment. A sequence of tomograms was collected from the lungs within the intact thoracic cavity. Digital volume correlation (DVC) was used to compute the three-dimensional strain field at the alveolar level from the time sequence of reconstructed tomograms. Regional differences in ventilation were highlighted during the respiratory cycle, relating the local strains within the lung tissue to the global ventilation measurements. Strains locally reached approximately 150% compared to the averaged regional deformations of approximately 80–100%. Redistribution of air within the lungs was observed during cycling. Regions which were relatively poorly ventilated (low deformations compared to its neighbouring region) were deforming more uniformly at later stages of the experiment (consistent with its neighbouring region). Such heterogenous phenomena are common in everyday breathing. In pathological lungs, some of these non-uniformities in deformation behaviour can become exaggerated, leading to poor function or further damage. The technique presented can help characterize the multiscale biomechanical nature of a given pathology to improve patient management strategies, considering both the local and global lung mechanics. MDPI 2021-01-18 /pmc/articles/PMC7829924/ /pubmed/33477444 http://dx.doi.org/10.3390/ma14020439 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Arora, Hari
Mitchell, Ria L.
Johnston, Richard
Manolesos, Marinos
Howells, David
Sherwood, Joseph M.
Bodey, Andrew J.
Wanelik, Kaz
Correlating Local Volumetric Tissue Strains with Global Lung Mechanics Measurements
title Correlating Local Volumetric Tissue Strains with Global Lung Mechanics Measurements
title_full Correlating Local Volumetric Tissue Strains with Global Lung Mechanics Measurements
title_fullStr Correlating Local Volumetric Tissue Strains with Global Lung Mechanics Measurements
title_full_unstemmed Correlating Local Volumetric Tissue Strains with Global Lung Mechanics Measurements
title_short Correlating Local Volumetric Tissue Strains with Global Lung Mechanics Measurements
title_sort correlating local volumetric tissue strains with global lung mechanics measurements
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829924/
https://www.ncbi.nlm.nih.gov/pubmed/33477444
http://dx.doi.org/10.3390/ma14020439
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