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Evaluation of Antimicrobial Effect of Air-Polishing Treatments and Their Influence on Human Dental Pulp Stem Cells Seeded on Titanium Disks

Dental implants are one of the most frequently used treatment options for tooth replacement, and titanium is the metal of choice due to its demonstrated superiority in resisting corrosion, lack of allergic reactions and mechanical strength. Surface roughness of titanium implants favors the osseointe...

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Autores principales: Di Tinco, Rosanna, Bertani, Giulia, Pisciotta, Alessandra, Bertoni, Laura, Bertacchini, Jessika, Colombari, Bruna, Conserva, Enrico, Blasi, Elisabetta, Consolo, Ugo, Carnevale, Gianluca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830275/
https://www.ncbi.nlm.nih.gov/pubmed/33467097
http://dx.doi.org/10.3390/ijms22020865
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author Di Tinco, Rosanna
Bertani, Giulia
Pisciotta, Alessandra
Bertoni, Laura
Bertacchini, Jessika
Colombari, Bruna
Conserva, Enrico
Blasi, Elisabetta
Consolo, Ugo
Carnevale, Gianluca
author_facet Di Tinco, Rosanna
Bertani, Giulia
Pisciotta, Alessandra
Bertoni, Laura
Bertacchini, Jessika
Colombari, Bruna
Conserva, Enrico
Blasi, Elisabetta
Consolo, Ugo
Carnevale, Gianluca
author_sort Di Tinco, Rosanna
collection PubMed
description Dental implants are one of the most frequently used treatment options for tooth replacement, and titanium is the metal of choice due to its demonstrated superiority in resisting corrosion, lack of allergic reactions and mechanical strength. Surface roughness of titanium implants favors the osseointegration process; nevertheless, its topography may provide a suitable substrate for bacterial biofilm deposition, causing peri-implantitis and leading to implant failure. Subgingival prophylaxis treatments with cleansing powders aimed to remove the bacterial accumulation are under investigation. Two different air-polishing powders—glycine and tagatose—were assayed for their cleaning and antimicrobial potential against a Pseudomonas biofilm and for their effects on human dental pulp stem cells (hDPSCs), seeded on sandblasted titanium disks. Immunofluorescence analyses were carried out to evaluate cell adhesion, proliferation, stemness and osteogenic differentiation. The results demonstrate that both the powders have a great in vitro cleaning potential in the early period and do not show any negative effects during hDPSCs osteogenic differentiation process, suggesting their suitability for enhancing the biocompatibility of titanium implants. Our data suggest that the evaluated cleansing systems reduce microbial contamination and allow us to propose tagatose as an adequate alternative to the gold standard glycine for the air-polishing prophylaxis treatment.
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spelling pubmed-78302752021-01-26 Evaluation of Antimicrobial Effect of Air-Polishing Treatments and Their Influence on Human Dental Pulp Stem Cells Seeded on Titanium Disks Di Tinco, Rosanna Bertani, Giulia Pisciotta, Alessandra Bertoni, Laura Bertacchini, Jessika Colombari, Bruna Conserva, Enrico Blasi, Elisabetta Consolo, Ugo Carnevale, Gianluca Int J Mol Sci Article Dental implants are one of the most frequently used treatment options for tooth replacement, and titanium is the metal of choice due to its demonstrated superiority in resisting corrosion, lack of allergic reactions and mechanical strength. Surface roughness of titanium implants favors the osseointegration process; nevertheless, its topography may provide a suitable substrate for bacterial biofilm deposition, causing peri-implantitis and leading to implant failure. Subgingival prophylaxis treatments with cleansing powders aimed to remove the bacterial accumulation are under investigation. Two different air-polishing powders—glycine and tagatose—were assayed for their cleaning and antimicrobial potential against a Pseudomonas biofilm and for their effects on human dental pulp stem cells (hDPSCs), seeded on sandblasted titanium disks. Immunofluorescence analyses were carried out to evaluate cell adhesion, proliferation, stemness and osteogenic differentiation. The results demonstrate that both the powders have a great in vitro cleaning potential in the early period and do not show any negative effects during hDPSCs osteogenic differentiation process, suggesting their suitability for enhancing the biocompatibility of titanium implants. Our data suggest that the evaluated cleansing systems reduce microbial contamination and allow us to propose tagatose as an adequate alternative to the gold standard glycine for the air-polishing prophylaxis treatment. MDPI 2021-01-16 /pmc/articles/PMC7830275/ /pubmed/33467097 http://dx.doi.org/10.3390/ijms22020865 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Di Tinco, Rosanna
Bertani, Giulia
Pisciotta, Alessandra
Bertoni, Laura
Bertacchini, Jessika
Colombari, Bruna
Conserva, Enrico
Blasi, Elisabetta
Consolo, Ugo
Carnevale, Gianluca
Evaluation of Antimicrobial Effect of Air-Polishing Treatments and Their Influence on Human Dental Pulp Stem Cells Seeded on Titanium Disks
title Evaluation of Antimicrobial Effect of Air-Polishing Treatments and Their Influence on Human Dental Pulp Stem Cells Seeded on Titanium Disks
title_full Evaluation of Antimicrobial Effect of Air-Polishing Treatments and Their Influence on Human Dental Pulp Stem Cells Seeded on Titanium Disks
title_fullStr Evaluation of Antimicrobial Effect of Air-Polishing Treatments and Their Influence on Human Dental Pulp Stem Cells Seeded on Titanium Disks
title_full_unstemmed Evaluation of Antimicrobial Effect of Air-Polishing Treatments and Their Influence on Human Dental Pulp Stem Cells Seeded on Titanium Disks
title_short Evaluation of Antimicrobial Effect of Air-Polishing Treatments and Their Influence on Human Dental Pulp Stem Cells Seeded on Titanium Disks
title_sort evaluation of antimicrobial effect of air-polishing treatments and their influence on human dental pulp stem cells seeded on titanium disks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830275/
https://www.ncbi.nlm.nih.gov/pubmed/33467097
http://dx.doi.org/10.3390/ijms22020865
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