Cargando…

Self-Collection of Saliva Specimens as a Suitable Alternative to Nasopharyngeal Swabs for the Diagnosis of SARS-CoV-2 by RT-qPCR

A nasopharyngeal swab is a sample used for the diagnosis of SARS-CoV-2 infection. Saliva is a sample easier to obtain and the risk of contagion for the professional is lower. This study aimed to evaluate the utility of saliva for the diagnosis of SARS-CoV-2 infection. This prospective study involved...

Descripción completa

Detalles Bibliográficos
Autores principales: Trobajo-Sanmartín, Camino, Adelantado, Marta, Navascués, Ana, Guembe, María J., Rodrigo-Rincón, Isabel, Castilla, Jesús, Ezpeleta, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830328/
https://www.ncbi.nlm.nih.gov/pubmed/33467501
http://dx.doi.org/10.3390/jcm10020299
_version_ 1783641385945006080
author Trobajo-Sanmartín, Camino
Adelantado, Marta
Navascués, Ana
Guembe, María J.
Rodrigo-Rincón, Isabel
Castilla, Jesús
Ezpeleta, Carmen
author_facet Trobajo-Sanmartín, Camino
Adelantado, Marta
Navascués, Ana
Guembe, María J.
Rodrigo-Rincón, Isabel
Castilla, Jesús
Ezpeleta, Carmen
author_sort Trobajo-Sanmartín, Camino
collection PubMed
description A nasopharyngeal swab is a sample used for the diagnosis of SARS-CoV-2 infection. Saliva is a sample easier to obtain and the risk of contagion for the professional is lower. This study aimed to evaluate the utility of saliva for the diagnosis of SARS-CoV-2 infection. This prospective study involved 674 patients with suspected SARS-CoV-2 infection. Paired nasopharyngeal and saliva samples were processed by RT-qPCR. Sensitivity, specificity, and kappa coefficient were used to evaluate the results from both samples. We considered the influence of age, symptoms, chronic conditions, and sample processing with lysis buffer. Of the 674 patients, 636 (94.4%) had valid results from both samples. The virus detection in saliva compared to a nasopharyngeal sample (gold standard) was 51.9% (95% CI: 46.3%–57.4%) and increased to 91.6% (95% CI: 86.7%–96.5%) when the cycle threshold (Ct) was ≤ 30. The specificity of the saliva sample was 99.1% (95% CI: 97.0%–99.8%). The concordance between samples was 75% (κ = 0.50; 95% CI: 0.45–0.56). The Ct values were significantly higher in saliva. In conclusion, saliva sample utility is limited for clinical diagnosis, but could be a useful alternative for the detection of SARS-CoV-2 in massive screening studies, when the availability of trained professionals for sampling or personal protection equipment is limited.
format Online
Article
Text
id pubmed-7830328
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-78303282021-01-26 Self-Collection of Saliva Specimens as a Suitable Alternative to Nasopharyngeal Swabs for the Diagnosis of SARS-CoV-2 by RT-qPCR Trobajo-Sanmartín, Camino Adelantado, Marta Navascués, Ana Guembe, María J. Rodrigo-Rincón, Isabel Castilla, Jesús Ezpeleta, Carmen J Clin Med Article A nasopharyngeal swab is a sample used for the diagnosis of SARS-CoV-2 infection. Saliva is a sample easier to obtain and the risk of contagion for the professional is lower. This study aimed to evaluate the utility of saliva for the diagnosis of SARS-CoV-2 infection. This prospective study involved 674 patients with suspected SARS-CoV-2 infection. Paired nasopharyngeal and saliva samples were processed by RT-qPCR. Sensitivity, specificity, and kappa coefficient were used to evaluate the results from both samples. We considered the influence of age, symptoms, chronic conditions, and sample processing with lysis buffer. Of the 674 patients, 636 (94.4%) had valid results from both samples. The virus detection in saliva compared to a nasopharyngeal sample (gold standard) was 51.9% (95% CI: 46.3%–57.4%) and increased to 91.6% (95% CI: 86.7%–96.5%) when the cycle threshold (Ct) was ≤ 30. The specificity of the saliva sample was 99.1% (95% CI: 97.0%–99.8%). The concordance between samples was 75% (κ = 0.50; 95% CI: 0.45–0.56). The Ct values were significantly higher in saliva. In conclusion, saliva sample utility is limited for clinical diagnosis, but could be a useful alternative for the detection of SARS-CoV-2 in massive screening studies, when the availability of trained professionals for sampling or personal protection equipment is limited. MDPI 2021-01-15 /pmc/articles/PMC7830328/ /pubmed/33467501 http://dx.doi.org/10.3390/jcm10020299 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Trobajo-Sanmartín, Camino
Adelantado, Marta
Navascués, Ana
Guembe, María J.
Rodrigo-Rincón, Isabel
Castilla, Jesús
Ezpeleta, Carmen
Self-Collection of Saliva Specimens as a Suitable Alternative to Nasopharyngeal Swabs for the Diagnosis of SARS-CoV-2 by RT-qPCR
title Self-Collection of Saliva Specimens as a Suitable Alternative to Nasopharyngeal Swabs for the Diagnosis of SARS-CoV-2 by RT-qPCR
title_full Self-Collection of Saliva Specimens as a Suitable Alternative to Nasopharyngeal Swabs for the Diagnosis of SARS-CoV-2 by RT-qPCR
title_fullStr Self-Collection of Saliva Specimens as a Suitable Alternative to Nasopharyngeal Swabs for the Diagnosis of SARS-CoV-2 by RT-qPCR
title_full_unstemmed Self-Collection of Saliva Specimens as a Suitable Alternative to Nasopharyngeal Swabs for the Diagnosis of SARS-CoV-2 by RT-qPCR
title_short Self-Collection of Saliva Specimens as a Suitable Alternative to Nasopharyngeal Swabs for the Diagnosis of SARS-CoV-2 by RT-qPCR
title_sort self-collection of saliva specimens as a suitable alternative to nasopharyngeal swabs for the diagnosis of sars-cov-2 by rt-qpcr
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830328/
https://www.ncbi.nlm.nih.gov/pubmed/33467501
http://dx.doi.org/10.3390/jcm10020299
work_keys_str_mv AT trobajosanmartincamino selfcollectionofsalivaspecimensasasuitablealternativetonasopharyngealswabsforthediagnosisofsarscov2byrtqpcr
AT adelantadomarta selfcollectionofsalivaspecimensasasuitablealternativetonasopharyngealswabsforthediagnosisofsarscov2byrtqpcr
AT navascuesana selfcollectionofsalivaspecimensasasuitablealternativetonasopharyngealswabsforthediagnosisofsarscov2byrtqpcr
AT guembemariaj selfcollectionofsalivaspecimensasasuitablealternativetonasopharyngealswabsforthediagnosisofsarscov2byrtqpcr
AT rodrigorinconisabel selfcollectionofsalivaspecimensasasuitablealternativetonasopharyngealswabsforthediagnosisofsarscov2byrtqpcr
AT castillajesus selfcollectionofsalivaspecimensasasuitablealternativetonasopharyngealswabsforthediagnosisofsarscov2byrtqpcr
AT ezpeletacarmen selfcollectionofsalivaspecimensasasuitablealternativetonasopharyngealswabsforthediagnosisofsarscov2byrtqpcr