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The Influence of Melatonin and Light on VEGF Secretion in Primary RPE Cells

(1) Background: Retinal pigment epithelial cells (RPE) cells constitutively secrete vascular endothelial growth factor (VEGF) in the retina, protecting the neuronal cells and the choroid. Increased VEGF secretion, however, can result in neovascularization and edema. Many factors regulate VEGF secret...

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Autores principales: Klettner, Alexa, Kampers, Miriam, Töbelmann, Daniela, Roider, Johann, Dittmar, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830335/
https://www.ncbi.nlm.nih.gov/pubmed/33467052
http://dx.doi.org/10.3390/biom11010114
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author Klettner, Alexa
Kampers, Miriam
Töbelmann, Daniela
Roider, Johann
Dittmar, Manuela
author_facet Klettner, Alexa
Kampers, Miriam
Töbelmann, Daniela
Roider, Johann
Dittmar, Manuela
author_sort Klettner, Alexa
collection PubMed
description (1) Background: Retinal pigment epithelial cells (RPE) cells constitutively secrete vascular endothelial growth factor (VEGF) in the retina, protecting the neuronal cells and the choroid. Increased VEGF secretion, however, can result in neovascularization and edema. Many factors regulate VEGF secretion. In this study, we investigated the effect of external stimuli in relation to diurnal rhythm on constitutive VEGF secretion. (2) Methods: Single-eye RPE cell culture was prepared from porcine eyes. RPE cells were cultured in darkness, treated with daylight or room light, and treated with melatonin at different time frames, either respectively or in combination. Supernatants were collected and VEGF content evaluated using ELISA. Expression of the clock protein BMAL1 was evaluated with Western blot. (3) Results: VEGF secretion of the RPE shows a diurnal rhythm. While the rhythm is not influenced by either light or melatonin, the amount of secreted VEGF can be increased by nocturnal melatonin, especially in combination with morning daylight. These findings disclose another layer of VEGF regulation in the retina.
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spelling pubmed-78303352021-01-26 The Influence of Melatonin and Light on VEGF Secretion in Primary RPE Cells Klettner, Alexa Kampers, Miriam Töbelmann, Daniela Roider, Johann Dittmar, Manuela Biomolecules Article (1) Background: Retinal pigment epithelial cells (RPE) cells constitutively secrete vascular endothelial growth factor (VEGF) in the retina, protecting the neuronal cells and the choroid. Increased VEGF secretion, however, can result in neovascularization and edema. Many factors regulate VEGF secretion. In this study, we investigated the effect of external stimuli in relation to diurnal rhythm on constitutive VEGF secretion. (2) Methods: Single-eye RPE cell culture was prepared from porcine eyes. RPE cells were cultured in darkness, treated with daylight or room light, and treated with melatonin at different time frames, either respectively or in combination. Supernatants were collected and VEGF content evaluated using ELISA. Expression of the clock protein BMAL1 was evaluated with Western blot. (3) Results: VEGF secretion of the RPE shows a diurnal rhythm. While the rhythm is not influenced by either light or melatonin, the amount of secreted VEGF can be increased by nocturnal melatonin, especially in combination with morning daylight. These findings disclose another layer of VEGF regulation in the retina. MDPI 2021-01-16 /pmc/articles/PMC7830335/ /pubmed/33467052 http://dx.doi.org/10.3390/biom11010114 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Klettner, Alexa
Kampers, Miriam
Töbelmann, Daniela
Roider, Johann
Dittmar, Manuela
The Influence of Melatonin and Light on VEGF Secretion in Primary RPE Cells
title The Influence of Melatonin and Light on VEGF Secretion in Primary RPE Cells
title_full The Influence of Melatonin and Light on VEGF Secretion in Primary RPE Cells
title_fullStr The Influence of Melatonin and Light on VEGF Secretion in Primary RPE Cells
title_full_unstemmed The Influence of Melatonin and Light on VEGF Secretion in Primary RPE Cells
title_short The Influence of Melatonin and Light on VEGF Secretion in Primary RPE Cells
title_sort influence of melatonin and light on vegf secretion in primary rpe cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830335/
https://www.ncbi.nlm.nih.gov/pubmed/33467052
http://dx.doi.org/10.3390/biom11010114
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