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High Integrity and Fidelity of Long-Term Cryopreserved Umbilical Cord Blood for Transplantation
Umbilical cord blood (UCB) is used as a source of donor cells for hematopoietic stem cell (HSC) transplantation. The success of transplantation is dependent on the quality of cord blood (CB) units for maximizing the chance of engraftment. Improved outcomes following transplantation are associated wi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830419/ https://www.ncbi.nlm.nih.gov/pubmed/33466868 http://dx.doi.org/10.3390/jcm10020293 |
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author | Kim, Gee-Hye Kwak, Jihye Kim, Sung Hee Kim, Hee Jung Hong, Hye Kyung Jin, Hye Jin Choi, Soo Jin Oh, Wonil Um, Soyoun |
author_facet | Kim, Gee-Hye Kwak, Jihye Kim, Sung Hee Kim, Hee Jung Hong, Hye Kyung Jin, Hye Jin Choi, Soo Jin Oh, Wonil Um, Soyoun |
author_sort | Kim, Gee-Hye |
collection | PubMed |
description | Umbilical cord blood (UCB) is used as a source of donor cells for hematopoietic stem cell (HSC) transplantation. The success of transplantation is dependent on the quality of cord blood (CB) units for maximizing the chance of engraftment. Improved outcomes following transplantation are associated with certain factors of cryopreserved CB units: total volume and total nucleated cell (TNC) count, mononuclear cell (MNC) count, and CD34+ cell count. The role of the storage period of CB units in determining the viability and counts of cells is less clear and is related to the quality of cryopreserved CB units. Herein, we demonstrate the recovery of viable TNCs and CD34+ cells, as well as the MNC viability in 20-year-old cryopreserved CB units in a CB bank (MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do, Korea). In addition, cell populations in CB units were evaluated for future clinical applications. The stable recovery rate of the viability of cryopreserved CB that had been stored for up to 20 years suggested the possibility of uses of the long-term cryopreservation of CB units. Similar relationships were observed in the recovery of TNCs and CD34+ cells in units of cryopreserved and fresh CB. The high-viability recovery of long-term cryopreserved CB suggests that successful hematopoietic stem cell (HSC) transplantation and other clinical applications, which are suitable for treating incurable diseases, may be performed regardless of long-term storage. |
format | Online Article Text |
id | pubmed-7830419 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78304192021-01-26 High Integrity and Fidelity of Long-Term Cryopreserved Umbilical Cord Blood for Transplantation Kim, Gee-Hye Kwak, Jihye Kim, Sung Hee Kim, Hee Jung Hong, Hye Kyung Jin, Hye Jin Choi, Soo Jin Oh, Wonil Um, Soyoun J Clin Med Article Umbilical cord blood (UCB) is used as a source of donor cells for hematopoietic stem cell (HSC) transplantation. The success of transplantation is dependent on the quality of cord blood (CB) units for maximizing the chance of engraftment. Improved outcomes following transplantation are associated with certain factors of cryopreserved CB units: total volume and total nucleated cell (TNC) count, mononuclear cell (MNC) count, and CD34+ cell count. The role of the storage period of CB units in determining the viability and counts of cells is less clear and is related to the quality of cryopreserved CB units. Herein, we demonstrate the recovery of viable TNCs and CD34+ cells, as well as the MNC viability in 20-year-old cryopreserved CB units in a CB bank (MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do, Korea). In addition, cell populations in CB units were evaluated for future clinical applications. The stable recovery rate of the viability of cryopreserved CB that had been stored for up to 20 years suggested the possibility of uses of the long-term cryopreservation of CB units. Similar relationships were observed in the recovery of TNCs and CD34+ cells in units of cryopreserved and fresh CB. The high-viability recovery of long-term cryopreserved CB suggests that successful hematopoietic stem cell (HSC) transplantation and other clinical applications, which are suitable for treating incurable diseases, may be performed regardless of long-term storage. MDPI 2021-01-14 /pmc/articles/PMC7830419/ /pubmed/33466868 http://dx.doi.org/10.3390/jcm10020293 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Gee-Hye Kwak, Jihye Kim, Sung Hee Kim, Hee Jung Hong, Hye Kyung Jin, Hye Jin Choi, Soo Jin Oh, Wonil Um, Soyoun High Integrity and Fidelity of Long-Term Cryopreserved Umbilical Cord Blood for Transplantation |
title | High Integrity and Fidelity of Long-Term Cryopreserved Umbilical Cord Blood for Transplantation |
title_full | High Integrity and Fidelity of Long-Term Cryopreserved Umbilical Cord Blood for Transplantation |
title_fullStr | High Integrity and Fidelity of Long-Term Cryopreserved Umbilical Cord Blood for Transplantation |
title_full_unstemmed | High Integrity and Fidelity of Long-Term Cryopreserved Umbilical Cord Blood for Transplantation |
title_short | High Integrity and Fidelity of Long-Term Cryopreserved Umbilical Cord Blood for Transplantation |
title_sort | high integrity and fidelity of long-term cryopreserved umbilical cord blood for transplantation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830419/ https://www.ncbi.nlm.nih.gov/pubmed/33466868 http://dx.doi.org/10.3390/jcm10020293 |
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