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Blastocystis sp. Prevalence and Subtypes Distribution amongst Syrian Refugee Communities Living in North Lebanon
Molecular data concerning the prevalence and subtype (ST) distribution of the intestinal parasite Blastocystis sp. remain scarce in the Middle East. Accordingly, we performed the first molecular epidemiological survey ever conducted in the Syrian population. A total of 306 stool samples were collect...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830609/ https://www.ncbi.nlm.nih.gov/pubmed/33467077 http://dx.doi.org/10.3390/microorganisms9010184 |
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author | Khaled, Salma Gantois, Nausicaa Ayoubi, Aisha Even, Gaël Sawant, Manasi El Houmayraa, Jinane Nabot, Mathieu Benamrouz-Vanneste, Sadia Chabé, Magali Certad, Gabriela El Safadi, Dima Dabboussi, Fouad Hamze, Monzer Viscogliosi, Eric |
author_facet | Khaled, Salma Gantois, Nausicaa Ayoubi, Aisha Even, Gaël Sawant, Manasi El Houmayraa, Jinane Nabot, Mathieu Benamrouz-Vanneste, Sadia Chabé, Magali Certad, Gabriela El Safadi, Dima Dabboussi, Fouad Hamze, Monzer Viscogliosi, Eric |
author_sort | Khaled, Salma |
collection | PubMed |
description | Molecular data concerning the prevalence and subtype (ST) distribution of the intestinal parasite Blastocystis sp. remain scarce in the Middle East. Accordingly, we performed the first molecular epidemiological survey ever conducted in the Syrian population. A total of 306 stool samples were collected from Syrian refugees living in 26 informal tented settlements (ITS) subjected or not to water, sanitation, and hygiene (WASH) interventions in North Lebanon, then screened for the presence of Blastocystis sp. by real-time polymerase chain reaction followed by subtyping. The overall prevalence of the parasite was shown to reach 63.7%. Blastocystis sp. colonization was not significantly associated with gender, age, symptomatic status, abdominal pain or diarrhea. In contrast, WASH intervention status of ITS was identified as a risk factor for infection. Among a total of 164 subtyped isolates, ST3 was predominant, followed by ST1, ST2, and ST10. No particular ST was reported to be associated with age, gender, symptomatic status, digestive disorders, or WASH intervention status of ITS. Intra-ST diversity of ST1 to ST3 was low suggesting large-scale anthroponotic transmission. Moreover, comparative analysis of ST1 to ST3 genotypes revealed that the circulation of the parasite between Syrian refugees and the host population was likely limited. |
format | Online Article Text |
id | pubmed-7830609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78306092021-01-26 Blastocystis sp. Prevalence and Subtypes Distribution amongst Syrian Refugee Communities Living in North Lebanon Khaled, Salma Gantois, Nausicaa Ayoubi, Aisha Even, Gaël Sawant, Manasi El Houmayraa, Jinane Nabot, Mathieu Benamrouz-Vanneste, Sadia Chabé, Magali Certad, Gabriela El Safadi, Dima Dabboussi, Fouad Hamze, Monzer Viscogliosi, Eric Microorganisms Article Molecular data concerning the prevalence and subtype (ST) distribution of the intestinal parasite Blastocystis sp. remain scarce in the Middle East. Accordingly, we performed the first molecular epidemiological survey ever conducted in the Syrian population. A total of 306 stool samples were collected from Syrian refugees living in 26 informal tented settlements (ITS) subjected or not to water, sanitation, and hygiene (WASH) interventions in North Lebanon, then screened for the presence of Blastocystis sp. by real-time polymerase chain reaction followed by subtyping. The overall prevalence of the parasite was shown to reach 63.7%. Blastocystis sp. colonization was not significantly associated with gender, age, symptomatic status, abdominal pain or diarrhea. In contrast, WASH intervention status of ITS was identified as a risk factor for infection. Among a total of 164 subtyped isolates, ST3 was predominant, followed by ST1, ST2, and ST10. No particular ST was reported to be associated with age, gender, symptomatic status, digestive disorders, or WASH intervention status of ITS. Intra-ST diversity of ST1 to ST3 was low suggesting large-scale anthroponotic transmission. Moreover, comparative analysis of ST1 to ST3 genotypes revealed that the circulation of the parasite between Syrian refugees and the host population was likely limited. MDPI 2021-01-16 /pmc/articles/PMC7830609/ /pubmed/33467077 http://dx.doi.org/10.3390/microorganisms9010184 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Khaled, Salma Gantois, Nausicaa Ayoubi, Aisha Even, Gaël Sawant, Manasi El Houmayraa, Jinane Nabot, Mathieu Benamrouz-Vanneste, Sadia Chabé, Magali Certad, Gabriela El Safadi, Dima Dabboussi, Fouad Hamze, Monzer Viscogliosi, Eric Blastocystis sp. Prevalence and Subtypes Distribution amongst Syrian Refugee Communities Living in North Lebanon |
title | Blastocystis sp. Prevalence and Subtypes Distribution amongst Syrian Refugee Communities Living in North Lebanon |
title_full | Blastocystis sp. Prevalence and Subtypes Distribution amongst Syrian Refugee Communities Living in North Lebanon |
title_fullStr | Blastocystis sp. Prevalence and Subtypes Distribution amongst Syrian Refugee Communities Living in North Lebanon |
title_full_unstemmed | Blastocystis sp. Prevalence and Subtypes Distribution amongst Syrian Refugee Communities Living in North Lebanon |
title_short | Blastocystis sp. Prevalence and Subtypes Distribution amongst Syrian Refugee Communities Living in North Lebanon |
title_sort | blastocystis sp. prevalence and subtypes distribution amongst syrian refugee communities living in north lebanon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830609/ https://www.ncbi.nlm.nih.gov/pubmed/33467077 http://dx.doi.org/10.3390/microorganisms9010184 |
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