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Muscari comosum L. Bulb Extracts Modulate Oxidative Stress and Redox Signaling in HepG2 Cells

Muscari comosum L. bulbs are commonly used as food in South Italy and also in folk medicine. By evaluating in vitro antioxidant activity and biological activities of their aqueous and methanol extracts, we shed light on the potential role, including both the nutraceutical and health benefits, of thi...

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Autores principales: Giglio, Fabiana, Castiglione Morelli, Maria Antonietta, Matera, Ilenia, Sinisgalli, Chiara, Rossano, Rocco, Ostuni, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830645/
https://www.ncbi.nlm.nih.gov/pubmed/33466890
http://dx.doi.org/10.3390/molecules26020416
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author Giglio, Fabiana
Castiglione Morelli, Maria Antonietta
Matera, Ilenia
Sinisgalli, Chiara
Rossano, Rocco
Ostuni, Angela
author_facet Giglio, Fabiana
Castiglione Morelli, Maria Antonietta
Matera, Ilenia
Sinisgalli, Chiara
Rossano, Rocco
Ostuni, Angela
author_sort Giglio, Fabiana
collection PubMed
description Muscari comosum L. bulbs are commonly used as food in South Italy and also in folk medicine. By evaluating in vitro antioxidant activity and biological activities of their aqueous and methanol extracts, we shed light on the potential role, including both the nutraceutical and health benefits, of this plant. Total polyphenol content (TPC) and total flavonoid content (TFC) were evaluated by the Folin–Ciocalteu method and by the aluminum chloride method, respectively. Antioxidant activity was investigated by three in vitro assays and relative antioxidant capacity index (RACI) was calculated to compare results obtained by different tests. The extracts were tested to evaluate their possible involvement in redox homeostasis, using the human hepatoma (HepG2) cell line used as model. The extracts exhibited concentration/solvent dependent radical scavenging activity, as well as dysregulation of some genes involved in redox pathways by promoting Nrf2, SOD-2, GPX1, ABCC6 and ABCG2 expression. NMR metabolomics analysis suggests that HepG2 cells treated with Muscari comosum extracts experience changes in some metabolites involved in various metabolic pathways.
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spelling pubmed-78306452021-01-26 Muscari comosum L. Bulb Extracts Modulate Oxidative Stress and Redox Signaling in HepG2 Cells Giglio, Fabiana Castiglione Morelli, Maria Antonietta Matera, Ilenia Sinisgalli, Chiara Rossano, Rocco Ostuni, Angela Molecules Article Muscari comosum L. bulbs are commonly used as food in South Italy and also in folk medicine. By evaluating in vitro antioxidant activity and biological activities of their aqueous and methanol extracts, we shed light on the potential role, including both the nutraceutical and health benefits, of this plant. Total polyphenol content (TPC) and total flavonoid content (TFC) were evaluated by the Folin–Ciocalteu method and by the aluminum chloride method, respectively. Antioxidant activity was investigated by three in vitro assays and relative antioxidant capacity index (RACI) was calculated to compare results obtained by different tests. The extracts were tested to evaluate their possible involvement in redox homeostasis, using the human hepatoma (HepG2) cell line used as model. The extracts exhibited concentration/solvent dependent radical scavenging activity, as well as dysregulation of some genes involved in redox pathways by promoting Nrf2, SOD-2, GPX1, ABCC6 and ABCG2 expression. NMR metabolomics analysis suggests that HepG2 cells treated with Muscari comosum extracts experience changes in some metabolites involved in various metabolic pathways. MDPI 2021-01-14 /pmc/articles/PMC7830645/ /pubmed/33466890 http://dx.doi.org/10.3390/molecules26020416 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Giglio, Fabiana
Castiglione Morelli, Maria Antonietta
Matera, Ilenia
Sinisgalli, Chiara
Rossano, Rocco
Ostuni, Angela
Muscari comosum L. Bulb Extracts Modulate Oxidative Stress and Redox Signaling in HepG2 Cells
title Muscari comosum L. Bulb Extracts Modulate Oxidative Stress and Redox Signaling in HepG2 Cells
title_full Muscari comosum L. Bulb Extracts Modulate Oxidative Stress and Redox Signaling in HepG2 Cells
title_fullStr Muscari comosum L. Bulb Extracts Modulate Oxidative Stress and Redox Signaling in HepG2 Cells
title_full_unstemmed Muscari comosum L. Bulb Extracts Modulate Oxidative Stress and Redox Signaling in HepG2 Cells
title_short Muscari comosum L. Bulb Extracts Modulate Oxidative Stress and Redox Signaling in HepG2 Cells
title_sort muscari comosum l. bulb extracts modulate oxidative stress and redox signaling in hepg2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830645/
https://www.ncbi.nlm.nih.gov/pubmed/33466890
http://dx.doi.org/10.3390/molecules26020416
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