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Circular RNA circMYL1 Inhibit Proliferation and Promote Differentiation of Myoblasts by Sponging miR-2400

Circular RNAs (circRNAs) are a class of endogenous non-coding RNAs (ncRNAs) involved in regulating skeletal muscle development by sponging miRNAs. In this study, we found that the circMYL1 expression was down-regulated during myoblast proliferation, while gradually up-regulated in myoblast different...

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Autores principales: Elnour, Ibrahim Elsaeid, Wang, Xiaogang, Zhansaya, Toremurat, Akhatayeva, Zhanerke, Khan, Rajwali, Cheng, Jie, Hung, Yongzhen, Lan, Xianyong, Lei, Chuzhao, Chen, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830797/
https://www.ncbi.nlm.nih.gov/pubmed/33467116
http://dx.doi.org/10.3390/cells10010176
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author Elnour, Ibrahim Elsaeid
Wang, Xiaogang
Zhansaya, Toremurat
Akhatayeva, Zhanerke
Khan, Rajwali
Cheng, Jie
Hung, Yongzhen
Lan, Xianyong
Lei, Chuzhao
Chen, Hong
author_facet Elnour, Ibrahim Elsaeid
Wang, Xiaogang
Zhansaya, Toremurat
Akhatayeva, Zhanerke
Khan, Rajwali
Cheng, Jie
Hung, Yongzhen
Lan, Xianyong
Lei, Chuzhao
Chen, Hong
author_sort Elnour, Ibrahim Elsaeid
collection PubMed
description Circular RNAs (circRNAs) are a class of endogenous non-coding RNAs (ncRNAs) involved in regulating skeletal muscle development by sponging miRNAs. In this study, we found that the circMYL1 expression was down-regulated during myoblast proliferation, while gradually up-regulated in myoblast differentiation. The potential role of circMYL1 was identified in the proliferation of bovine myoblast through mRNA and protein expression of proliferation marker genes (PCNA, CyclinD1, and CDK2), cell counting kit-8 assay, flow cytometry analysis, and 5-ethynyl 2′-deoxyuridine (EdU) assay. Analysis of the expression of differentiation marker genes (MyoD, MyoG, and MYH2) and immunofluorescence of Myosin heavy chain (MyHC) was used to assess cell differentiation. The proliferation analysis revealed that circMYL1 inhibited the proliferation of bovine primary myoblast. Furthermore, the differentiation analysis demonstrated that circMYL1 promoted the differentiation of bovine primary myoblast. The luciferase screening and RNA immunoprecipitation (RIP) assays found that circMYL1 could have interaction with miR-2400. Additionally, we demonstrated that miR-2400 promoted proliferation and inhibited differentiation of bovine primary myoblast, while circMYL1 may eliminate the effects of miR-2400, as showed by rescue experiments. Together, our results revealed that a novel circular RNA of circMYL1 could inhibit proliferation and promote differentiation of myoblast by sponging miR-2400.
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spelling pubmed-78307972021-01-26 Circular RNA circMYL1 Inhibit Proliferation and Promote Differentiation of Myoblasts by Sponging miR-2400 Elnour, Ibrahim Elsaeid Wang, Xiaogang Zhansaya, Toremurat Akhatayeva, Zhanerke Khan, Rajwali Cheng, Jie Hung, Yongzhen Lan, Xianyong Lei, Chuzhao Chen, Hong Cells Article Circular RNAs (circRNAs) are a class of endogenous non-coding RNAs (ncRNAs) involved in regulating skeletal muscle development by sponging miRNAs. In this study, we found that the circMYL1 expression was down-regulated during myoblast proliferation, while gradually up-regulated in myoblast differentiation. The potential role of circMYL1 was identified in the proliferation of bovine myoblast through mRNA and protein expression of proliferation marker genes (PCNA, CyclinD1, and CDK2), cell counting kit-8 assay, flow cytometry analysis, and 5-ethynyl 2′-deoxyuridine (EdU) assay. Analysis of the expression of differentiation marker genes (MyoD, MyoG, and MYH2) and immunofluorescence of Myosin heavy chain (MyHC) was used to assess cell differentiation. The proliferation analysis revealed that circMYL1 inhibited the proliferation of bovine primary myoblast. Furthermore, the differentiation analysis demonstrated that circMYL1 promoted the differentiation of bovine primary myoblast. The luciferase screening and RNA immunoprecipitation (RIP) assays found that circMYL1 could have interaction with miR-2400. Additionally, we demonstrated that miR-2400 promoted proliferation and inhibited differentiation of bovine primary myoblast, while circMYL1 may eliminate the effects of miR-2400, as showed by rescue experiments. Together, our results revealed that a novel circular RNA of circMYL1 could inhibit proliferation and promote differentiation of myoblast by sponging miR-2400. MDPI 2021-01-16 /pmc/articles/PMC7830797/ /pubmed/33467116 http://dx.doi.org/10.3390/cells10010176 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Elnour, Ibrahim Elsaeid
Wang, Xiaogang
Zhansaya, Toremurat
Akhatayeva, Zhanerke
Khan, Rajwali
Cheng, Jie
Hung, Yongzhen
Lan, Xianyong
Lei, Chuzhao
Chen, Hong
Circular RNA circMYL1 Inhibit Proliferation and Promote Differentiation of Myoblasts by Sponging miR-2400
title Circular RNA circMYL1 Inhibit Proliferation and Promote Differentiation of Myoblasts by Sponging miR-2400
title_full Circular RNA circMYL1 Inhibit Proliferation and Promote Differentiation of Myoblasts by Sponging miR-2400
title_fullStr Circular RNA circMYL1 Inhibit Proliferation and Promote Differentiation of Myoblasts by Sponging miR-2400
title_full_unstemmed Circular RNA circMYL1 Inhibit Proliferation and Promote Differentiation of Myoblasts by Sponging miR-2400
title_short Circular RNA circMYL1 Inhibit Proliferation and Promote Differentiation of Myoblasts by Sponging miR-2400
title_sort circular rna circmyl1 inhibit proliferation and promote differentiation of myoblasts by sponging mir-2400
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830797/
https://www.ncbi.nlm.nih.gov/pubmed/33467116
http://dx.doi.org/10.3390/cells10010176
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