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In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens

Chronic wounds complicated with biofilm formed by pathogens remain one of the most significant challenges of contemporary medicine. The application of topical antiseptic solutions against wound biofilm has been gaining increasing interest among clinical practitioners and scientific researchers. This...

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Autores principales: Krasowski, Grzegorz, Junka, Adam, Paleczny, Justyna, Czajkowska, Joanna, Makomaska-Szaroszyk, Elżbieta, Chodaczek, Grzegorz, Majkowski, Michał, Migdał, Paweł, Fijałkowski, Karol, Kowalska-Krochmal, Beata, Bartoszewicz, Marzenna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830887/
https://www.ncbi.nlm.nih.gov/pubmed/33477349
http://dx.doi.org/10.3390/membranes11010062
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author Krasowski, Grzegorz
Junka, Adam
Paleczny, Justyna
Czajkowska, Joanna
Makomaska-Szaroszyk, Elżbieta
Chodaczek, Grzegorz
Majkowski, Michał
Migdał, Paweł
Fijałkowski, Karol
Kowalska-Krochmal, Beata
Bartoszewicz, Marzenna
author_facet Krasowski, Grzegorz
Junka, Adam
Paleczny, Justyna
Czajkowska, Joanna
Makomaska-Szaroszyk, Elżbieta
Chodaczek, Grzegorz
Majkowski, Michał
Migdał, Paweł
Fijałkowski, Karol
Kowalska-Krochmal, Beata
Bartoszewicz, Marzenna
author_sort Krasowski, Grzegorz
collection PubMed
description Chronic wounds complicated with biofilm formed by pathogens remain one of the most significant challenges of contemporary medicine. The application of topical antiseptic solutions against wound biofilm has been gaining increasing interest among clinical practitioners and scientific researchers. This paper compares the activity of polyhexanide-, octenidine- and hypochlorite/hypochlorous acid-based antiseptics against biofilm formed by clinical strains of Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa. The analyses included both standard techniques utilizing polystyrene plates and self-designed biocellulose-based models in which a biofilm formed by pathogens was formed on an elastic, fibrinous surface covered with a fibroblast layer. The obtained results show high antibiofilm activity of polihexanide- and octenidine-based antiseptics and lack or weak antibiofilm activity of hypochlorite-based antiseptic of total chlorine content equal to 80 parts per million. The data presented in this paper indicate that polihexanide- or octenidine-based antiseptics are highly useful in the treatment of biofilm, while hypochlorite-based antiseptics with low chlorine content may be applied for wound rinsing but not when specific antibiofilm activity is required.
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spelling pubmed-78308872021-01-26 In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens Krasowski, Grzegorz Junka, Adam Paleczny, Justyna Czajkowska, Joanna Makomaska-Szaroszyk, Elżbieta Chodaczek, Grzegorz Majkowski, Michał Migdał, Paweł Fijałkowski, Karol Kowalska-Krochmal, Beata Bartoszewicz, Marzenna Membranes (Basel) Article Chronic wounds complicated with biofilm formed by pathogens remain one of the most significant challenges of contemporary medicine. The application of topical antiseptic solutions against wound biofilm has been gaining increasing interest among clinical practitioners and scientific researchers. This paper compares the activity of polyhexanide-, octenidine- and hypochlorite/hypochlorous acid-based antiseptics against biofilm formed by clinical strains of Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa. The analyses included both standard techniques utilizing polystyrene plates and self-designed biocellulose-based models in which a biofilm formed by pathogens was formed on an elastic, fibrinous surface covered with a fibroblast layer. The obtained results show high antibiofilm activity of polihexanide- and octenidine-based antiseptics and lack or weak antibiofilm activity of hypochlorite-based antiseptic of total chlorine content equal to 80 parts per million. The data presented in this paper indicate that polihexanide- or octenidine-based antiseptics are highly useful in the treatment of biofilm, while hypochlorite-based antiseptics with low chlorine content may be applied for wound rinsing but not when specific antibiofilm activity is required. MDPI 2021-01-17 /pmc/articles/PMC7830887/ /pubmed/33477349 http://dx.doi.org/10.3390/membranes11010062 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krasowski, Grzegorz
Junka, Adam
Paleczny, Justyna
Czajkowska, Joanna
Makomaska-Szaroszyk, Elżbieta
Chodaczek, Grzegorz
Majkowski, Michał
Migdał, Paweł
Fijałkowski, Karol
Kowalska-Krochmal, Beata
Bartoszewicz, Marzenna
In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens
title In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens
title_full In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens
title_fullStr In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens
title_full_unstemmed In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens
title_short In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens
title_sort in vitro evaluation of polihexanide, octenidine and naclo/hclo-based antiseptics against biofilm formed by wound pathogens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830887/
https://www.ncbi.nlm.nih.gov/pubmed/33477349
http://dx.doi.org/10.3390/membranes11010062
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