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CD112 Regulates Angiogenesis and T Cell Entry into the Spleen
Junctional adhesion proteins play important roles in controlling angiogenesis, vascular permeability and leukocyte trafficking. CD112 (nectin-2) belongs to the immunoglobulin superfamily and was shown to engage in homophilic and heterophilic interactions with a variety of binding partners expressed...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830896/ https://www.ncbi.nlm.nih.gov/pubmed/33467729 http://dx.doi.org/10.3390/cells10010169 |
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author | Russo, Erica Runge, Peter Haghayegh Jahromi, Neda Naboth, Heidi Landtwing, Angela Montecchi, Riccardo Leicht, Noémie Hunter, Morgan Campbell Takai, Yoshimi Halin, Cornelia |
author_facet | Russo, Erica Runge, Peter Haghayegh Jahromi, Neda Naboth, Heidi Landtwing, Angela Montecchi, Riccardo Leicht, Noémie Hunter, Morgan Campbell Takai, Yoshimi Halin, Cornelia |
author_sort | Russo, Erica |
collection | PubMed |
description | Junctional adhesion proteins play important roles in controlling angiogenesis, vascular permeability and leukocyte trafficking. CD112 (nectin-2) belongs to the immunoglobulin superfamily and was shown to engage in homophilic and heterophilic interactions with a variety of binding partners expressed on endothelial cells and on leukocytes. Recent in vitro studies suggested that CD112 regulates human endothelial cell migration and proliferation as well as transendothelial migration of leukocytes. However, so far, the role of CD112 in endothelial cell biology and in leukocyte trafficking has not been elucidated in vivo. We found CD112 to be expressed by lymphatic and blood endothelial cells in different murine tissues. In CD112-deficient mice, the blood vessel coverage in the retina and spleen was significantly enhanced. In functional in vitro studies, a blockade of CD112 modulated endothelial cell migration and significantly enhanced endothelial tube formation. An antibody-based blockade of CD112 also significantly reduced T cell transmigration across endothelial monolayers in vitro. Moreover, T cell homing to the spleen was significantly reduced in CD112-deficient mice. Overall, our results identify CD112 as a regulator of angiogenic processes in vivo and demonstrate a novel role for CD112 in T cell entry into the spleen. |
format | Online Article Text |
id | pubmed-7830896 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78308962021-01-26 CD112 Regulates Angiogenesis and T Cell Entry into the Spleen Russo, Erica Runge, Peter Haghayegh Jahromi, Neda Naboth, Heidi Landtwing, Angela Montecchi, Riccardo Leicht, Noémie Hunter, Morgan Campbell Takai, Yoshimi Halin, Cornelia Cells Article Junctional adhesion proteins play important roles in controlling angiogenesis, vascular permeability and leukocyte trafficking. CD112 (nectin-2) belongs to the immunoglobulin superfamily and was shown to engage in homophilic and heterophilic interactions with a variety of binding partners expressed on endothelial cells and on leukocytes. Recent in vitro studies suggested that CD112 regulates human endothelial cell migration and proliferation as well as transendothelial migration of leukocytes. However, so far, the role of CD112 in endothelial cell biology and in leukocyte trafficking has not been elucidated in vivo. We found CD112 to be expressed by lymphatic and blood endothelial cells in different murine tissues. In CD112-deficient mice, the blood vessel coverage in the retina and spleen was significantly enhanced. In functional in vitro studies, a blockade of CD112 modulated endothelial cell migration and significantly enhanced endothelial tube formation. An antibody-based blockade of CD112 also significantly reduced T cell transmigration across endothelial monolayers in vitro. Moreover, T cell homing to the spleen was significantly reduced in CD112-deficient mice. Overall, our results identify CD112 as a regulator of angiogenic processes in vivo and demonstrate a novel role for CD112 in T cell entry into the spleen. MDPI 2021-01-15 /pmc/articles/PMC7830896/ /pubmed/33467729 http://dx.doi.org/10.3390/cells10010169 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Russo, Erica Runge, Peter Haghayegh Jahromi, Neda Naboth, Heidi Landtwing, Angela Montecchi, Riccardo Leicht, Noémie Hunter, Morgan Campbell Takai, Yoshimi Halin, Cornelia CD112 Regulates Angiogenesis and T Cell Entry into the Spleen |
title | CD112 Regulates Angiogenesis and T Cell Entry into the Spleen |
title_full | CD112 Regulates Angiogenesis and T Cell Entry into the Spleen |
title_fullStr | CD112 Regulates Angiogenesis and T Cell Entry into the Spleen |
title_full_unstemmed | CD112 Regulates Angiogenesis and T Cell Entry into the Spleen |
title_short | CD112 Regulates Angiogenesis and T Cell Entry into the Spleen |
title_sort | cd112 regulates angiogenesis and t cell entry into the spleen |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830896/ https://www.ncbi.nlm.nih.gov/pubmed/33467729 http://dx.doi.org/10.3390/cells10010169 |
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