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CD112 Regulates Angiogenesis and T Cell Entry into the Spleen

Junctional adhesion proteins play important roles in controlling angiogenesis, vascular permeability and leukocyte trafficking. CD112 (nectin-2) belongs to the immunoglobulin superfamily and was shown to engage in homophilic and heterophilic interactions with a variety of binding partners expressed...

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Autores principales: Russo, Erica, Runge, Peter, Haghayegh Jahromi, Neda, Naboth, Heidi, Landtwing, Angela, Montecchi, Riccardo, Leicht, Noémie, Hunter, Morgan Campbell, Takai, Yoshimi, Halin, Cornelia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830896/
https://www.ncbi.nlm.nih.gov/pubmed/33467729
http://dx.doi.org/10.3390/cells10010169
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author Russo, Erica
Runge, Peter
Haghayegh Jahromi, Neda
Naboth, Heidi
Landtwing, Angela
Montecchi, Riccardo
Leicht, Noémie
Hunter, Morgan Campbell
Takai, Yoshimi
Halin, Cornelia
author_facet Russo, Erica
Runge, Peter
Haghayegh Jahromi, Neda
Naboth, Heidi
Landtwing, Angela
Montecchi, Riccardo
Leicht, Noémie
Hunter, Morgan Campbell
Takai, Yoshimi
Halin, Cornelia
author_sort Russo, Erica
collection PubMed
description Junctional adhesion proteins play important roles in controlling angiogenesis, vascular permeability and leukocyte trafficking. CD112 (nectin-2) belongs to the immunoglobulin superfamily and was shown to engage in homophilic and heterophilic interactions with a variety of binding partners expressed on endothelial cells and on leukocytes. Recent in vitro studies suggested that CD112 regulates human endothelial cell migration and proliferation as well as transendothelial migration of leukocytes. However, so far, the role of CD112 in endothelial cell biology and in leukocyte trafficking has not been elucidated in vivo. We found CD112 to be expressed by lymphatic and blood endothelial cells in different murine tissues. In CD112-deficient mice, the blood vessel coverage in the retina and spleen was significantly enhanced. In functional in vitro studies, a blockade of CD112 modulated endothelial cell migration and significantly enhanced endothelial tube formation. An antibody-based blockade of CD112 also significantly reduced T cell transmigration across endothelial monolayers in vitro. Moreover, T cell homing to the spleen was significantly reduced in CD112-deficient mice. Overall, our results identify CD112 as a regulator of angiogenic processes in vivo and demonstrate a novel role for CD112 in T cell entry into the spleen.
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spelling pubmed-78308962021-01-26 CD112 Regulates Angiogenesis and T Cell Entry into the Spleen Russo, Erica Runge, Peter Haghayegh Jahromi, Neda Naboth, Heidi Landtwing, Angela Montecchi, Riccardo Leicht, Noémie Hunter, Morgan Campbell Takai, Yoshimi Halin, Cornelia Cells Article Junctional adhesion proteins play important roles in controlling angiogenesis, vascular permeability and leukocyte trafficking. CD112 (nectin-2) belongs to the immunoglobulin superfamily and was shown to engage in homophilic and heterophilic interactions with a variety of binding partners expressed on endothelial cells and on leukocytes. Recent in vitro studies suggested that CD112 regulates human endothelial cell migration and proliferation as well as transendothelial migration of leukocytes. However, so far, the role of CD112 in endothelial cell biology and in leukocyte trafficking has not been elucidated in vivo. We found CD112 to be expressed by lymphatic and blood endothelial cells in different murine tissues. In CD112-deficient mice, the blood vessel coverage in the retina and spleen was significantly enhanced. In functional in vitro studies, a blockade of CD112 modulated endothelial cell migration and significantly enhanced endothelial tube formation. An antibody-based blockade of CD112 also significantly reduced T cell transmigration across endothelial monolayers in vitro. Moreover, T cell homing to the spleen was significantly reduced in CD112-deficient mice. Overall, our results identify CD112 as a regulator of angiogenic processes in vivo and demonstrate a novel role for CD112 in T cell entry into the spleen. MDPI 2021-01-15 /pmc/articles/PMC7830896/ /pubmed/33467729 http://dx.doi.org/10.3390/cells10010169 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Russo, Erica
Runge, Peter
Haghayegh Jahromi, Neda
Naboth, Heidi
Landtwing, Angela
Montecchi, Riccardo
Leicht, Noémie
Hunter, Morgan Campbell
Takai, Yoshimi
Halin, Cornelia
CD112 Regulates Angiogenesis and T Cell Entry into the Spleen
title CD112 Regulates Angiogenesis and T Cell Entry into the Spleen
title_full CD112 Regulates Angiogenesis and T Cell Entry into the Spleen
title_fullStr CD112 Regulates Angiogenesis and T Cell Entry into the Spleen
title_full_unstemmed CD112 Regulates Angiogenesis and T Cell Entry into the Spleen
title_short CD112 Regulates Angiogenesis and T Cell Entry into the Spleen
title_sort cd112 regulates angiogenesis and t cell entry into the spleen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830896/
https://www.ncbi.nlm.nih.gov/pubmed/33467729
http://dx.doi.org/10.3390/cells10010169
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