Facile In-Situ Fabrication of a Ternary ZnO/TiO(2)/Ag Nanocomposite for Enhanced Bactericidal and Biocompatibility Properties

This paper presents for the first time a successful fabrication of ternary ZnO/TiO(2)/Ag nanocomposites consisting of zinc oxide (ZnO), titania (TiO(2)) and silver (Ag) nanoparticles (NPs) synthesised using Morinda citrifolia fruit (MCF) extract. ZnONPs were synthesised using the co-precipitation method, and TiO(2) and Ag were introduced into the precursor solutions under microwave irradiation to obtain ZnO/TiO(2)/Ag nanocomposites (NCs). This material demonstrated enhanced bactericidal effect towards bacterial pathogens compared to that of the binary TiO(2)/Ag, Ag and TiO(2) alone. In vitro cytotoxicity results of the as-synthesised ZnO/TiO(2)/AgNCs on RAW 264.7 macrophages and A549 cell lines revealed a negative role in cytotoxicity, but contributed astoundingly towards antimicrobials as compared of Ag alone and binary Ag/TiO(2). This study shows that the resultant ternary metal/bi-semiconductor nanocomposites may provide a therapeutic strategy for the eradication of bacterial pathogens without affecting the healthy mammalian cells.