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The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales
Polyphosphates (polyP) are polymers of orthophosphate residues linked by high-energy phosphoanhydride bonds that are important in all domains of life and function in many different processes, including biofilm development. To study the effect of polyP in archaeal biofilm formation, our previously de...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831078/ https://www.ncbi.nlm.nih.gov/pubmed/33477546 http://dx.doi.org/10.3390/microorganisms9010193 |
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author | Recalde, Alejandra van Wolferen, Marleen Sivabalasarma, Shamphavi Albers, Sonja-Verena Navarro, Claudio A. Jerez, Carlos A. |
author_facet | Recalde, Alejandra van Wolferen, Marleen Sivabalasarma, Shamphavi Albers, Sonja-Verena Navarro, Claudio A. Jerez, Carlos A. |
author_sort | Recalde, Alejandra |
collection | PubMed |
description | Polyphosphates (polyP) are polymers of orthophosphate residues linked by high-energy phosphoanhydride bonds that are important in all domains of life and function in many different processes, including biofilm development. To study the effect of polyP in archaeal biofilm formation, our previously described Sa. solfataricus polyP (−) strain and a new polyP (−) S. acidocaldarius strain generated in this report were used. These two strains lack the polymer due to the overexpression of their respective exopolyphosphatase gene (ppx). Both strains showed a reduction in biofilm formation, decreased motility on semi-solid plates and a diminished adherence to glass surfaces as seen by DAPI (4′,6-diamidino-2-phenylindole) staining using fluorescence microscopy. Even though arlB (encoding the archaellum subunit) was highly upregulated in S. acidocardarius polyP (−), no archaellated cells were observed. These results suggest that polyP might be involved in the regulation of the expression of archaellum components and their assembly, possibly by affecting energy availability, phosphorylation or other phenomena. This is the first evidence indicating polyP affects biofilm formation and other related processes in archaea. |
format | Online Article Text |
id | pubmed-7831078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78310782021-01-26 The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales Recalde, Alejandra van Wolferen, Marleen Sivabalasarma, Shamphavi Albers, Sonja-Verena Navarro, Claudio A. Jerez, Carlos A. Microorganisms Article Polyphosphates (polyP) are polymers of orthophosphate residues linked by high-energy phosphoanhydride bonds that are important in all domains of life and function in many different processes, including biofilm development. To study the effect of polyP in archaeal biofilm formation, our previously described Sa. solfataricus polyP (−) strain and a new polyP (−) S. acidocaldarius strain generated in this report were used. These two strains lack the polymer due to the overexpression of their respective exopolyphosphatase gene (ppx). Both strains showed a reduction in biofilm formation, decreased motility on semi-solid plates and a diminished adherence to glass surfaces as seen by DAPI (4′,6-diamidino-2-phenylindole) staining using fluorescence microscopy. Even though arlB (encoding the archaellum subunit) was highly upregulated in S. acidocardarius polyP (−), no archaellated cells were observed. These results suggest that polyP might be involved in the regulation of the expression of archaellum components and their assembly, possibly by affecting energy availability, phosphorylation or other phenomena. This is the first evidence indicating polyP affects biofilm formation and other related processes in archaea. MDPI 2021-01-18 /pmc/articles/PMC7831078/ /pubmed/33477546 http://dx.doi.org/10.3390/microorganisms9010193 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Recalde, Alejandra van Wolferen, Marleen Sivabalasarma, Shamphavi Albers, Sonja-Verena Navarro, Claudio A. Jerez, Carlos A. The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales |
title | The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales |
title_full | The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales |
title_fullStr | The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales |
title_full_unstemmed | The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales |
title_short | The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales |
title_sort | role of polyphosphate in motility, adhesion, and biofilm formation in sulfolobales |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831078/ https://www.ncbi.nlm.nih.gov/pubmed/33477546 http://dx.doi.org/10.3390/microorganisms9010193 |
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