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The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales

Polyphosphates (polyP) are polymers of orthophosphate residues linked by high-energy phosphoanhydride bonds that are important in all domains of life and function in many different processes, including biofilm development. To study the effect of polyP in archaeal biofilm formation, our previously de...

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Autores principales: Recalde, Alejandra, van Wolferen, Marleen, Sivabalasarma, Shamphavi, Albers, Sonja-Verena, Navarro, Claudio A., Jerez, Carlos A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831078/
https://www.ncbi.nlm.nih.gov/pubmed/33477546
http://dx.doi.org/10.3390/microorganisms9010193
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author Recalde, Alejandra
van Wolferen, Marleen
Sivabalasarma, Shamphavi
Albers, Sonja-Verena
Navarro, Claudio A.
Jerez, Carlos A.
author_facet Recalde, Alejandra
van Wolferen, Marleen
Sivabalasarma, Shamphavi
Albers, Sonja-Verena
Navarro, Claudio A.
Jerez, Carlos A.
author_sort Recalde, Alejandra
collection PubMed
description Polyphosphates (polyP) are polymers of orthophosphate residues linked by high-energy phosphoanhydride bonds that are important in all domains of life and function in many different processes, including biofilm development. To study the effect of polyP in archaeal biofilm formation, our previously described Sa. solfataricus polyP (−) strain and a new polyP (−) S. acidocaldarius strain generated in this report were used. These two strains lack the polymer due to the overexpression of their respective exopolyphosphatase gene (ppx). Both strains showed a reduction in biofilm formation, decreased motility on semi-solid plates and a diminished adherence to glass surfaces as seen by DAPI (4′,6-diamidino-2-phenylindole) staining using fluorescence microscopy. Even though arlB (encoding the archaellum subunit) was highly upregulated in S. acidocardarius polyP (−), no archaellated cells were observed. These results suggest that polyP might be involved in the regulation of the expression of archaellum components and their assembly, possibly by affecting energy availability, phosphorylation or other phenomena. This is the first evidence indicating polyP affects biofilm formation and other related processes in archaea.
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spelling pubmed-78310782021-01-26 The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales Recalde, Alejandra van Wolferen, Marleen Sivabalasarma, Shamphavi Albers, Sonja-Verena Navarro, Claudio A. Jerez, Carlos A. Microorganisms Article Polyphosphates (polyP) are polymers of orthophosphate residues linked by high-energy phosphoanhydride bonds that are important in all domains of life and function in many different processes, including biofilm development. To study the effect of polyP in archaeal biofilm formation, our previously described Sa. solfataricus polyP (−) strain and a new polyP (−) S. acidocaldarius strain generated in this report were used. These two strains lack the polymer due to the overexpression of their respective exopolyphosphatase gene (ppx). Both strains showed a reduction in biofilm formation, decreased motility on semi-solid plates and a diminished adherence to glass surfaces as seen by DAPI (4′,6-diamidino-2-phenylindole) staining using fluorescence microscopy. Even though arlB (encoding the archaellum subunit) was highly upregulated in S. acidocardarius polyP (−), no archaellated cells were observed. These results suggest that polyP might be involved in the regulation of the expression of archaellum components and their assembly, possibly by affecting energy availability, phosphorylation or other phenomena. This is the first evidence indicating polyP affects biofilm formation and other related processes in archaea. MDPI 2021-01-18 /pmc/articles/PMC7831078/ /pubmed/33477546 http://dx.doi.org/10.3390/microorganisms9010193 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Recalde, Alejandra
van Wolferen, Marleen
Sivabalasarma, Shamphavi
Albers, Sonja-Verena
Navarro, Claudio A.
Jerez, Carlos A.
The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales
title The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales
title_full The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales
title_fullStr The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales
title_full_unstemmed The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales
title_short The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales
title_sort role of polyphosphate in motility, adhesion, and biofilm formation in sulfolobales
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831078/
https://www.ncbi.nlm.nih.gov/pubmed/33477546
http://dx.doi.org/10.3390/microorganisms9010193
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