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Dysregulated H3K27 Acetylation Is Implicated in Fatty Liver Hemorrhagic Syndrome in Chickens

Epigenetic regulation of gene expression has been reported in the pathogenesis of metabolic disorders such as diabetes and liver steatosis in humans. However, the molecular mechanisms of fatty liver hemorrhagic syndrome (FLHS) in chickens have been rarely studied. H3K27ac chromatin immunoprecipitati...

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Autores principales: Zhu, Yaling, Zeng, Qingjie, Li, Fang, Fang, Haoshu, Zhou, Zhimin, Jiang, Tao, Yin, Chao, Wei, Qing, Wang, Yujie, Ruan, Jiming, Huang, Jianzhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831272/
https://www.ncbi.nlm.nih.gov/pubmed/33505421
http://dx.doi.org/10.3389/fgene.2020.574167
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author Zhu, Yaling
Zeng, Qingjie
Li, Fang
Fang, Haoshu
Zhou, Zhimin
Jiang, Tao
Yin, Chao
Wei, Qing
Wang, Yujie
Ruan, Jiming
Huang, Jianzhen
author_facet Zhu, Yaling
Zeng, Qingjie
Li, Fang
Fang, Haoshu
Zhou, Zhimin
Jiang, Tao
Yin, Chao
Wei, Qing
Wang, Yujie
Ruan, Jiming
Huang, Jianzhen
author_sort Zhu, Yaling
collection PubMed
description Epigenetic regulation of gene expression has been reported in the pathogenesis of metabolic disorders such as diabetes and liver steatosis in humans. However, the molecular mechanisms of fatty liver hemorrhagic syndrome (FLHS) in chickens have been rarely studied. H3K27ac chromatin immunoprecipitation coupled with high-throughput sequencing and high-throughput RNA sequencing was performed to compare genome-wide H3K27ac profiles and transcriptomes of liver tissue between healthy and FLHS chickens. In total, 1,321 differential H3K27ac regions and 443 differentially expressed genes were identified (| log2Fold change| ≥ 1 and P-value ≤ 0.05) between the two groups. Binding motifs for transcription factors involved in immune processes and metabolic homeostasis were enriched among those differential H3K27ac regions. Differential H3K27ac peaks were associated with multiple known FLHS risk genes, involved in lipid and energy metabolism (PCK1, APOA1, ANGPTL4, and FABP1) and the immune system (FGF7, PDGFRA, and KIT). Previous studies and our current results suggested that the high-energy, low-protein (HELP) diet might have an impact on histone modification and chromatin structure, leading to the dysregulation of candidate genes and the peroxisome proliferator-activated receptor (PPAR) signaling pathway, which causes excessive accumulation of fat in the liver tissue and induces the development of FLHS. These findings highlight that epigenetic modifications contribute to the regulation of gene expression and play a central regulatory role in FLHS. The PPAR signaling pathway and other genes implicated in FLHS are of great importance for the development of novel and specific therapies for FLHS-susceptible commercial laying hens.
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spelling pubmed-78312722021-01-26 Dysregulated H3K27 Acetylation Is Implicated in Fatty Liver Hemorrhagic Syndrome in Chickens Zhu, Yaling Zeng, Qingjie Li, Fang Fang, Haoshu Zhou, Zhimin Jiang, Tao Yin, Chao Wei, Qing Wang, Yujie Ruan, Jiming Huang, Jianzhen Front Genet Genetics Epigenetic regulation of gene expression has been reported in the pathogenesis of metabolic disorders such as diabetes and liver steatosis in humans. However, the molecular mechanisms of fatty liver hemorrhagic syndrome (FLHS) in chickens have been rarely studied. H3K27ac chromatin immunoprecipitation coupled with high-throughput sequencing and high-throughput RNA sequencing was performed to compare genome-wide H3K27ac profiles and transcriptomes of liver tissue between healthy and FLHS chickens. In total, 1,321 differential H3K27ac regions and 443 differentially expressed genes were identified (| log2Fold change| ≥ 1 and P-value ≤ 0.05) between the two groups. Binding motifs for transcription factors involved in immune processes and metabolic homeostasis were enriched among those differential H3K27ac regions. Differential H3K27ac peaks were associated with multiple known FLHS risk genes, involved in lipid and energy metabolism (PCK1, APOA1, ANGPTL4, and FABP1) and the immune system (FGF7, PDGFRA, and KIT). Previous studies and our current results suggested that the high-energy, low-protein (HELP) diet might have an impact on histone modification and chromatin structure, leading to the dysregulation of candidate genes and the peroxisome proliferator-activated receptor (PPAR) signaling pathway, which causes excessive accumulation of fat in the liver tissue and induces the development of FLHS. These findings highlight that epigenetic modifications contribute to the regulation of gene expression and play a central regulatory role in FLHS. The PPAR signaling pathway and other genes implicated in FLHS are of great importance for the development of novel and specific therapies for FLHS-susceptible commercial laying hens. Frontiers Media S.A. 2021-01-11 /pmc/articles/PMC7831272/ /pubmed/33505421 http://dx.doi.org/10.3389/fgene.2020.574167 Text en Copyright © 2021 Zhu, Zeng, Li, Fang, Zhou, Jiang, Yin, Wei, Wang, Ruan and Huang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zhu, Yaling
Zeng, Qingjie
Li, Fang
Fang, Haoshu
Zhou, Zhimin
Jiang, Tao
Yin, Chao
Wei, Qing
Wang, Yujie
Ruan, Jiming
Huang, Jianzhen
Dysregulated H3K27 Acetylation Is Implicated in Fatty Liver Hemorrhagic Syndrome in Chickens
title Dysregulated H3K27 Acetylation Is Implicated in Fatty Liver Hemorrhagic Syndrome in Chickens
title_full Dysregulated H3K27 Acetylation Is Implicated in Fatty Liver Hemorrhagic Syndrome in Chickens
title_fullStr Dysregulated H3K27 Acetylation Is Implicated in Fatty Liver Hemorrhagic Syndrome in Chickens
title_full_unstemmed Dysregulated H3K27 Acetylation Is Implicated in Fatty Liver Hemorrhagic Syndrome in Chickens
title_short Dysregulated H3K27 Acetylation Is Implicated in Fatty Liver Hemorrhagic Syndrome in Chickens
title_sort dysregulated h3k27 acetylation is implicated in fatty liver hemorrhagic syndrome in chickens
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831272/
https://www.ncbi.nlm.nih.gov/pubmed/33505421
http://dx.doi.org/10.3389/fgene.2020.574167
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