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Quantitative Proteomics Reveals that GmENO2 Proteins Are Involved in Response to Phosphate Starvation in the Leaves of Glycine max L.
Soybean (Glycine max L.) is a major crop providing important source for protein and oil for human life. Low phosphate (LP) availability is a critical limiting factor affecting soybean production. Soybean plants develop a series of strategies to adapt to phosphate (Pi) limitation condition. However,...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831476/ https://www.ncbi.nlm.nih.gov/pubmed/33477636 http://dx.doi.org/10.3390/ijms22020920 |
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author | Cheng, Ling Min, Wanling Li, Man Zhou, Lili Hsu, Chuan-Chih Yang, Xuelian Jiang, Xue Ruan, Zhijie Zhong, Yongjia Wang, Zhi-Yong Wang, Wenfei |
author_facet | Cheng, Ling Min, Wanling Li, Man Zhou, Lili Hsu, Chuan-Chih Yang, Xuelian Jiang, Xue Ruan, Zhijie Zhong, Yongjia Wang, Zhi-Yong Wang, Wenfei |
author_sort | Cheng, Ling |
collection | PubMed |
description | Soybean (Glycine max L.) is a major crop providing important source for protein and oil for human life. Low phosphate (LP) availability is a critical limiting factor affecting soybean production. Soybean plants develop a series of strategies to adapt to phosphate (Pi) limitation condition. However, the underlying molecular mechanisms responsible for LP stress response remain largely unknown. Here, we performed a label-free quantification (LFQ) analysis of soybean leaves grown under low and high phosphate conditions. We identified 267 induced and 440 reduced differential proteins from phosphate-starved leaves. Almost a quarter of the LP decreased proteins are involved in translation processes, while the LP increased proteins are accumulated in chlorophyll biosynthetic and carbon metabolic processes. Among these induced proteins, an enolase protein, GmENO2a was found to be mostly induced protein. On the transcriptional level, GmENO2a and GmENO2b, but not GmENO2c or GmENO2d, were dramatically induced by phosphate starvation. Among 14 enolase genes, only GmENO2a and GmENO2b genes contain the P1BS motif in their promoter regions. Furthermore, GmENO2b was specifically induced in the GmPHR31 overexpressing soybean plants. Our findings provide molecular insights into how soybean plants tune basic carbon metabolic pathway to adapt to Pi deprivation through the ENO2 enzymes. |
format | Online Article Text |
id | pubmed-7831476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78314762021-01-26 Quantitative Proteomics Reveals that GmENO2 Proteins Are Involved in Response to Phosphate Starvation in the Leaves of Glycine max L. Cheng, Ling Min, Wanling Li, Man Zhou, Lili Hsu, Chuan-Chih Yang, Xuelian Jiang, Xue Ruan, Zhijie Zhong, Yongjia Wang, Zhi-Yong Wang, Wenfei Int J Mol Sci Article Soybean (Glycine max L.) is a major crop providing important source for protein and oil for human life. Low phosphate (LP) availability is a critical limiting factor affecting soybean production. Soybean plants develop a series of strategies to adapt to phosphate (Pi) limitation condition. However, the underlying molecular mechanisms responsible for LP stress response remain largely unknown. Here, we performed a label-free quantification (LFQ) analysis of soybean leaves grown under low and high phosphate conditions. We identified 267 induced and 440 reduced differential proteins from phosphate-starved leaves. Almost a quarter of the LP decreased proteins are involved in translation processes, while the LP increased proteins are accumulated in chlorophyll biosynthetic and carbon metabolic processes. Among these induced proteins, an enolase protein, GmENO2a was found to be mostly induced protein. On the transcriptional level, GmENO2a and GmENO2b, but not GmENO2c or GmENO2d, were dramatically induced by phosphate starvation. Among 14 enolase genes, only GmENO2a and GmENO2b genes contain the P1BS motif in their promoter regions. Furthermore, GmENO2b was specifically induced in the GmPHR31 overexpressing soybean plants. Our findings provide molecular insights into how soybean plants tune basic carbon metabolic pathway to adapt to Pi deprivation through the ENO2 enzymes. MDPI 2021-01-18 /pmc/articles/PMC7831476/ /pubmed/33477636 http://dx.doi.org/10.3390/ijms22020920 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cheng, Ling Min, Wanling Li, Man Zhou, Lili Hsu, Chuan-Chih Yang, Xuelian Jiang, Xue Ruan, Zhijie Zhong, Yongjia Wang, Zhi-Yong Wang, Wenfei Quantitative Proteomics Reveals that GmENO2 Proteins Are Involved in Response to Phosphate Starvation in the Leaves of Glycine max L. |
title | Quantitative Proteomics Reveals that GmENO2 Proteins Are Involved in Response to Phosphate Starvation in the Leaves of Glycine max L. |
title_full | Quantitative Proteomics Reveals that GmENO2 Proteins Are Involved in Response to Phosphate Starvation in the Leaves of Glycine max L. |
title_fullStr | Quantitative Proteomics Reveals that GmENO2 Proteins Are Involved in Response to Phosphate Starvation in the Leaves of Glycine max L. |
title_full_unstemmed | Quantitative Proteomics Reveals that GmENO2 Proteins Are Involved in Response to Phosphate Starvation in the Leaves of Glycine max L. |
title_short | Quantitative Proteomics Reveals that GmENO2 Proteins Are Involved in Response to Phosphate Starvation in the Leaves of Glycine max L. |
title_sort | quantitative proteomics reveals that gmeno2 proteins are involved in response to phosphate starvation in the leaves of glycine max l. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831476/ https://www.ncbi.nlm.nih.gov/pubmed/33477636 http://dx.doi.org/10.3390/ijms22020920 |
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