Cargando…

An Integrated Transcriptome Analysis Reveals IGFBP7 Upregulation in Vasculature in Traumatic Brain Injury

Vasculature plays critical roles in the pathogenesis and neurological repair of traumatic brain injury (TBI). However, how vascular endothelial cells respond to TBI at the molecular level has not been systematically reviewed. Here, by integrating three transcriptome datasets including whole cortex o...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jianhao, Deng, Xiangyi, Xie, Yuan, Tang, Jiefu, Zhou, Ziwei, Yang, Fan, He, Qiyuan, Cao, Qingze, Zhang, Lei, He, Liqun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831608/
https://www.ncbi.nlm.nih.gov/pubmed/33505428
http://dx.doi.org/10.3389/fgene.2020.599834
_version_ 1783641653228077056
author Wang, Jianhao
Deng, Xiangyi
Xie, Yuan
Tang, Jiefu
Zhou, Ziwei
Yang, Fan
He, Qiyuan
Cao, Qingze
Zhang, Lei
He, Liqun
author_facet Wang, Jianhao
Deng, Xiangyi
Xie, Yuan
Tang, Jiefu
Zhou, Ziwei
Yang, Fan
He, Qiyuan
Cao, Qingze
Zhang, Lei
He, Liqun
author_sort Wang, Jianhao
collection PubMed
description Vasculature plays critical roles in the pathogenesis and neurological repair of traumatic brain injury (TBI). However, how vascular endothelial cells respond to TBI at the molecular level has not been systematically reviewed. Here, by integrating three transcriptome datasets including whole cortex of mouse brain, FACS-sorted mouse brain endothelial cells, and single cell sequencing of mouse brain hippocampus, we revealed the key molecular alteration of endothelial cells characterized by increased Myc targets and Epithelial-Mesenchymal Transition signatures. In addition, immunofluorescence staining of patients’ samples confirmed that IGFBP7 was up-regulated in vasculature in response to TBI. TGFβ1, mainly derived from microglia and endothelial cells, sufficiently induces IGFBP7 expression in cultured endothelial cells, and is significantly upregulated in response to TBI. Our results identified IGFBP7 as a potential biomarker of vasculature in response to TBI, and indicate that TGFβ signaling may contribute to the upregulation of IGFBP7 in the vasculature.
format Online
Article
Text
id pubmed-7831608
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-78316082021-01-26 An Integrated Transcriptome Analysis Reveals IGFBP7 Upregulation in Vasculature in Traumatic Brain Injury Wang, Jianhao Deng, Xiangyi Xie, Yuan Tang, Jiefu Zhou, Ziwei Yang, Fan He, Qiyuan Cao, Qingze Zhang, Lei He, Liqun Front Genet Genetics Vasculature plays critical roles in the pathogenesis and neurological repair of traumatic brain injury (TBI). However, how vascular endothelial cells respond to TBI at the molecular level has not been systematically reviewed. Here, by integrating three transcriptome datasets including whole cortex of mouse brain, FACS-sorted mouse brain endothelial cells, and single cell sequencing of mouse brain hippocampus, we revealed the key molecular alteration of endothelial cells characterized by increased Myc targets and Epithelial-Mesenchymal Transition signatures. In addition, immunofluorescence staining of patients’ samples confirmed that IGFBP7 was up-regulated in vasculature in response to TBI. TGFβ1, mainly derived from microglia and endothelial cells, sufficiently induces IGFBP7 expression in cultured endothelial cells, and is significantly upregulated in response to TBI. Our results identified IGFBP7 as a potential biomarker of vasculature in response to TBI, and indicate that TGFβ signaling may contribute to the upregulation of IGFBP7 in the vasculature. Frontiers Media S.A. 2021-01-11 /pmc/articles/PMC7831608/ /pubmed/33505428 http://dx.doi.org/10.3389/fgene.2020.599834 Text en Copyright © 2021 Wang, Deng, Xie, Tang, Zhou, Yang, He, Cao, Zhang and He. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Wang, Jianhao
Deng, Xiangyi
Xie, Yuan
Tang, Jiefu
Zhou, Ziwei
Yang, Fan
He, Qiyuan
Cao, Qingze
Zhang, Lei
He, Liqun
An Integrated Transcriptome Analysis Reveals IGFBP7 Upregulation in Vasculature in Traumatic Brain Injury
title An Integrated Transcriptome Analysis Reveals IGFBP7 Upregulation in Vasculature in Traumatic Brain Injury
title_full An Integrated Transcriptome Analysis Reveals IGFBP7 Upregulation in Vasculature in Traumatic Brain Injury
title_fullStr An Integrated Transcriptome Analysis Reveals IGFBP7 Upregulation in Vasculature in Traumatic Brain Injury
title_full_unstemmed An Integrated Transcriptome Analysis Reveals IGFBP7 Upregulation in Vasculature in Traumatic Brain Injury
title_short An Integrated Transcriptome Analysis Reveals IGFBP7 Upregulation in Vasculature in Traumatic Brain Injury
title_sort integrated transcriptome analysis reveals igfbp7 upregulation in vasculature in traumatic brain injury
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831608/
https://www.ncbi.nlm.nih.gov/pubmed/33505428
http://dx.doi.org/10.3389/fgene.2020.599834
work_keys_str_mv AT wangjianhao anintegratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT dengxiangyi anintegratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT xieyuan anintegratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT tangjiefu anintegratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT zhouziwei anintegratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT yangfan anintegratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT heqiyuan anintegratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT caoqingze anintegratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT zhanglei anintegratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT heliqun anintegratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT wangjianhao integratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT dengxiangyi integratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT xieyuan integratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT tangjiefu integratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT zhouziwei integratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT yangfan integratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT heqiyuan integratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT caoqingze integratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT zhanglei integratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury
AT heliqun integratedtranscriptomeanalysisrevealsigfbp7upregulationinvasculatureintraumaticbraininjury