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Specific determination of hepatitis B e antigen by antibodies targeting precore unique epitope facilitates clinical diagnosis and drug evaluation against hepatitis B virus infection
Hepatitis B e antigen (HBeAg) is a widely used marker both for chronic hepatitis B (CHB) clinical management and HBV-related basic research. However, due to its high amino acid sequence homology to hepatitis B core antigen (HBcAg), most of available anti-HBe antibodies are cross-reactive with HBcAg...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7832009/ https://www.ncbi.nlm.nih.gov/pubmed/33296295 http://dx.doi.org/10.1080/22221751.2020.1862631 |
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author | Wang, Shao-Juan Chen, Zi-Min Wei, Min Liu, Jia-Qi Li, Zong-Lin Shi, Tian-Shu Nian, Sheng Fu, Rao Wu, Yang-Tao Zhang, Ya-Li Wang, Ying-Bin Zhang, Tian-Ying Zhang, Jun Xiong, Jun-Hui Tong, Shu-Ping Ge, Sheng-Xiang Yuan, Quan Xia, Ning-Shao |
author_facet | Wang, Shao-Juan Chen, Zi-Min Wei, Min Liu, Jia-Qi Li, Zong-Lin Shi, Tian-Shu Nian, Sheng Fu, Rao Wu, Yang-Tao Zhang, Ya-Li Wang, Ying-Bin Zhang, Tian-Ying Zhang, Jun Xiong, Jun-Hui Tong, Shu-Ping Ge, Sheng-Xiang Yuan, Quan Xia, Ning-Shao |
author_sort | Wang, Shao-Juan |
collection | PubMed |
description | Hepatitis B e antigen (HBeAg) is a widely used marker both for chronic hepatitis B (CHB) clinical management and HBV-related basic research. However, due to its high amino acid sequence homology to hepatitis B core antigen (HBcAg), most of available anti-HBe antibodies are cross-reactive with HBcAg resulting in high interference against accurate measurement of the status and level of HBeAg. In the study, we generated several monoclonal antibodies (mAbs) targeting various epitopes on HBeAg and HBcAg. Among these mAbs, a novel mAb 16D9, which recognizes the SKLCLG (aa −10 to −5) motif on the N-terminal residues of HBeAg that is absent on HBcAg, exhibited excellent detection sensitivity and specificity in pairing with another 14A7 mAb targeting the HBeAg C-terminus (STLPETTVVRRRGR, aa141 to 154). Based on these two mAbs, we developed a novel chemiluminescent HBeAg immunoassay (NTR-HBeAg) which could detect HBeAg derived from various HBV genotypes. In contrast to widely used commercial assays, the NTR-HBeAg completely eliminated the cross-reactivity with secreted HBcAg from precore mutant (G1896A) virus in either cell culture or patient sera. The improved specificity of the NTR-HBeAg assay enables its applicability in cccDNA-targeting drug screening in cell culture systems and also provides an accurate tool for clinical HBeAg detection. |
format | Online Article Text |
id | pubmed-7832009 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-78320092021-02-02 Specific determination of hepatitis B e antigen by antibodies targeting precore unique epitope facilitates clinical diagnosis and drug evaluation against hepatitis B virus infection Wang, Shao-Juan Chen, Zi-Min Wei, Min Liu, Jia-Qi Li, Zong-Lin Shi, Tian-Shu Nian, Sheng Fu, Rao Wu, Yang-Tao Zhang, Ya-Li Wang, Ying-Bin Zhang, Tian-Ying Zhang, Jun Xiong, Jun-Hui Tong, Shu-Ping Ge, Sheng-Xiang Yuan, Quan Xia, Ning-Shao Emerg Microbes Infect Hepatitis Hepatitis B e antigen (HBeAg) is a widely used marker both for chronic hepatitis B (CHB) clinical management and HBV-related basic research. However, due to its high amino acid sequence homology to hepatitis B core antigen (HBcAg), most of available anti-HBe antibodies are cross-reactive with HBcAg resulting in high interference against accurate measurement of the status and level of HBeAg. In the study, we generated several monoclonal antibodies (mAbs) targeting various epitopes on HBeAg and HBcAg. Among these mAbs, a novel mAb 16D9, which recognizes the SKLCLG (aa −10 to −5) motif on the N-terminal residues of HBeAg that is absent on HBcAg, exhibited excellent detection sensitivity and specificity in pairing with another 14A7 mAb targeting the HBeAg C-terminus (STLPETTVVRRRGR, aa141 to 154). Based on these two mAbs, we developed a novel chemiluminescent HBeAg immunoassay (NTR-HBeAg) which could detect HBeAg derived from various HBV genotypes. In contrast to widely used commercial assays, the NTR-HBeAg completely eliminated the cross-reactivity with secreted HBcAg from precore mutant (G1896A) virus in either cell culture or patient sera. The improved specificity of the NTR-HBeAg assay enables its applicability in cccDNA-targeting drug screening in cell culture systems and also provides an accurate tool for clinical HBeAg detection. Taylor & Francis 2021-01-17 /pmc/articles/PMC7832009/ /pubmed/33296295 http://dx.doi.org/10.1080/22221751.2020.1862631 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Hepatitis Wang, Shao-Juan Chen, Zi-Min Wei, Min Liu, Jia-Qi Li, Zong-Lin Shi, Tian-Shu Nian, Sheng Fu, Rao Wu, Yang-Tao Zhang, Ya-Li Wang, Ying-Bin Zhang, Tian-Ying Zhang, Jun Xiong, Jun-Hui Tong, Shu-Ping Ge, Sheng-Xiang Yuan, Quan Xia, Ning-Shao Specific determination of hepatitis B e antigen by antibodies targeting precore unique epitope facilitates clinical diagnosis and drug evaluation against hepatitis B virus infection |
title | Specific determination of hepatitis B e antigen by antibodies targeting precore unique epitope facilitates clinical diagnosis and drug evaluation against hepatitis B virus infection |
title_full | Specific determination of hepatitis B e antigen by antibodies targeting precore unique epitope facilitates clinical diagnosis and drug evaluation against hepatitis B virus infection |
title_fullStr | Specific determination of hepatitis B e antigen by antibodies targeting precore unique epitope facilitates clinical diagnosis and drug evaluation against hepatitis B virus infection |
title_full_unstemmed | Specific determination of hepatitis B e antigen by antibodies targeting precore unique epitope facilitates clinical diagnosis and drug evaluation against hepatitis B virus infection |
title_short | Specific determination of hepatitis B e antigen by antibodies targeting precore unique epitope facilitates clinical diagnosis and drug evaluation against hepatitis B virus infection |
title_sort | specific determination of hepatitis b e antigen by antibodies targeting precore unique epitope facilitates clinical diagnosis and drug evaluation against hepatitis b virus infection |
topic | Hepatitis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7832009/ https://www.ncbi.nlm.nih.gov/pubmed/33296295 http://dx.doi.org/10.1080/22221751.2020.1862631 |
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