Cargando…

Hesperidin Interacts With CREB-BDNF Signaling Pathway to Suppress Pentylenetetrazole-Induced Convulsions in Zebrafish

Hesperidin (3,5,7-trihydroxyflavanone 7-rhamnoglucoside) is a β-7-rutinoside of hesperetin (4′-methoxy-3′,5,7-trihydroxyflavanone), abundantly found in citrus fruits and known to interact with various cellular pathways to show a variety of pharmacological effects. The present study was envisaged to...

Descripción completa

Detalles Bibliográficos
Autores principales: Sharma, Pallavi, Kumari, Savita, Sharma, Jatin, Purohit, Rituraj, Singh, Damanpreet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7832091/
https://www.ncbi.nlm.nih.gov/pubmed/33505312
http://dx.doi.org/10.3389/fphar.2020.607797
_version_ 1783641758282809344
author Sharma, Pallavi
Kumari, Savita
Sharma, Jatin
Purohit, Rituraj
Singh, Damanpreet
author_facet Sharma, Pallavi
Kumari, Savita
Sharma, Jatin
Purohit, Rituraj
Singh, Damanpreet
author_sort Sharma, Pallavi
collection PubMed
description Hesperidin (3,5,7-trihydroxyflavanone 7-rhamnoglucoside) is a β-7-rutinoside of hesperetin (4′-methoxy-3′,5,7-trihydroxyflavanone), abundantly found in citrus fruits and known to interact with various cellular pathways to show a variety of pharmacological effects. The present study was envisaged to understand the anticonvulsant effect of hesperidin in a zebrafish model of pentylenetetrazole (PTZ)-induced convulsions, with the support of in silico docking. Healthy zebrafish larvae were preincubated with hesperidin (1, 5, and 10 µM) for 1 h, before PTZ exposure. Hesperidin treatment significantly increased the seizure latency and minimized PTZ-induced hyperactive responses. A significant reduction in c-fos expression further supported the suppression of neuronal excitation following hesperidin incubation in the larvae exposed to PTZ. The treatment also modulated larval bdnf expression and reduced the expression of il-10. The results of in vivo studies were further supported by in silico docking analysis, which showed the affinity of hesperidin for the N-methyl-d-aspartate receptor, the gamma-aminobutyric acid receptor, Interleukin 10 and the TrkB receptor of brain-derived neurotrophic factor. The results concluded that hesperidin suppresses PTZ-mediated seizure in zebrafish larvae through interaction with the central CREB–BDNF pathway.
format Online
Article
Text
id pubmed-7832091
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-78320912021-01-26 Hesperidin Interacts With CREB-BDNF Signaling Pathway to Suppress Pentylenetetrazole-Induced Convulsions in Zebrafish Sharma, Pallavi Kumari, Savita Sharma, Jatin Purohit, Rituraj Singh, Damanpreet Front Pharmacol Pharmacology Hesperidin (3,5,7-trihydroxyflavanone 7-rhamnoglucoside) is a β-7-rutinoside of hesperetin (4′-methoxy-3′,5,7-trihydroxyflavanone), abundantly found in citrus fruits and known to interact with various cellular pathways to show a variety of pharmacological effects. The present study was envisaged to understand the anticonvulsant effect of hesperidin in a zebrafish model of pentylenetetrazole (PTZ)-induced convulsions, with the support of in silico docking. Healthy zebrafish larvae were preincubated with hesperidin (1, 5, and 10 µM) for 1 h, before PTZ exposure. Hesperidin treatment significantly increased the seizure latency and minimized PTZ-induced hyperactive responses. A significant reduction in c-fos expression further supported the suppression of neuronal excitation following hesperidin incubation in the larvae exposed to PTZ. The treatment also modulated larval bdnf expression and reduced the expression of il-10. The results of in vivo studies were further supported by in silico docking analysis, which showed the affinity of hesperidin for the N-methyl-d-aspartate receptor, the gamma-aminobutyric acid receptor, Interleukin 10 and the TrkB receptor of brain-derived neurotrophic factor. The results concluded that hesperidin suppresses PTZ-mediated seizure in zebrafish larvae through interaction with the central CREB–BDNF pathway. Frontiers Media S.A. 2021-01-11 /pmc/articles/PMC7832091/ /pubmed/33505312 http://dx.doi.org/10.3389/fphar.2020.607797 Text en Copyright © 2021 Singh, Sharma, Kumari, Sharma and Purohit http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Sharma, Pallavi
Kumari, Savita
Sharma, Jatin
Purohit, Rituraj
Singh, Damanpreet
Hesperidin Interacts With CREB-BDNF Signaling Pathway to Suppress Pentylenetetrazole-Induced Convulsions in Zebrafish
title Hesperidin Interacts With CREB-BDNF Signaling Pathway to Suppress Pentylenetetrazole-Induced Convulsions in Zebrafish
title_full Hesperidin Interacts With CREB-BDNF Signaling Pathway to Suppress Pentylenetetrazole-Induced Convulsions in Zebrafish
title_fullStr Hesperidin Interacts With CREB-BDNF Signaling Pathway to Suppress Pentylenetetrazole-Induced Convulsions in Zebrafish
title_full_unstemmed Hesperidin Interacts With CREB-BDNF Signaling Pathway to Suppress Pentylenetetrazole-Induced Convulsions in Zebrafish
title_short Hesperidin Interacts With CREB-BDNF Signaling Pathway to Suppress Pentylenetetrazole-Induced Convulsions in Zebrafish
title_sort hesperidin interacts with creb-bdnf signaling pathway to suppress pentylenetetrazole-induced convulsions in zebrafish
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7832091/
https://www.ncbi.nlm.nih.gov/pubmed/33505312
http://dx.doi.org/10.3389/fphar.2020.607797
work_keys_str_mv AT sharmapallavi hesperidininteractswithcrebbdnfsignalingpathwaytosuppresspentylenetetrazoleinducedconvulsionsinzebrafish
AT kumarisavita hesperidininteractswithcrebbdnfsignalingpathwaytosuppresspentylenetetrazoleinducedconvulsionsinzebrafish
AT sharmajatin hesperidininteractswithcrebbdnfsignalingpathwaytosuppresspentylenetetrazoleinducedconvulsionsinzebrafish
AT purohitrituraj hesperidininteractswithcrebbdnfsignalingpathwaytosuppresspentylenetetrazoleinducedconvulsionsinzebrafish
AT singhdamanpreet hesperidininteractswithcrebbdnfsignalingpathwaytosuppresspentylenetetrazoleinducedconvulsionsinzebrafish