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Cytotoxicity and proliferation evaluation on fibroblast after combining calcium hydroxide and ellagic acid

Calcium hydroxide induces chronic inflammation and pulp tissue necrosis due to its high pH value. Ellagic acid is an anti-inflammatory and antioxidant flavonoid. Therefore, the effect of combining calcium hydroxide and ellagic acid must be researched to reduce cell damage due to the application of c...

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Autores principales: Nirwana, Intan, Munadziroh, Elly, Yogiartono, R. Mohammad, Thiyagu, Candrika, Ying, Chong Sin, Dinaryanti, Aristika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7832190/
https://www.ncbi.nlm.nih.gov/pubmed/33532351
http://dx.doi.org/10.4103/japtr.JAPTR_154_20
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author Nirwana, Intan
Munadziroh, Elly
Yogiartono, R. Mohammad
Thiyagu, Candrika
Ying, Chong Sin
Dinaryanti, Aristika
author_facet Nirwana, Intan
Munadziroh, Elly
Yogiartono, R. Mohammad
Thiyagu, Candrika
Ying, Chong Sin
Dinaryanti, Aristika
author_sort Nirwana, Intan
collection PubMed
description Calcium hydroxide induces chronic inflammation and pulp tissue necrosis due to its high pH value. Ellagic acid is an anti-inflammatory and antioxidant flavonoid. Therefore, the effect of combining calcium hydroxide and ellagic acid must be researched to reduce cell damage due to the application of calcium hydroxide. The objective of the study was to determine the cytotoxicity and proliferation of fibroblasts after combining calcium hydroxide and ellagic acid with ratios of 99:1, 98:2, 97:3, 96:4, and 95:5. Calcium hydroxide and ellagic acid with different ratios were mixed with water and stirred. Rat gingival fibroblasts were prepared and incubated in two 96-well microplates. The control group and treatment groups (16 samples) were placed in the microplate and incubated for 1 and 3 days. An MTT assay test was performed, and the absorbance was observed using the ELISA reader with a wavelength of 540 nm. Following that, the cell viability was calculated. The results were tabulated and analyzed using a one-way ANOVA. For all treatment groups, the fibroblast cells showed a viability of higher than 50%. There was a significant increase (P < 0.05) in the fibroblast cell proliferation after combining calcium hydroxide and ellagic acid with ratios of 99:1 and 97:3. The combination of calcium hydroxide and ellagic acid is nontoxic. The treatment groups with ratios of 99:1 and 97:3 showed increased fibroblast cell proliferation.
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spelling pubmed-78321902021-02-01 Cytotoxicity and proliferation evaluation on fibroblast after combining calcium hydroxide and ellagic acid Nirwana, Intan Munadziroh, Elly Yogiartono, R. Mohammad Thiyagu, Candrika Ying, Chong Sin Dinaryanti, Aristika J Adv Pharm Technol Res Original Article Calcium hydroxide induces chronic inflammation and pulp tissue necrosis due to its high pH value. Ellagic acid is an anti-inflammatory and antioxidant flavonoid. Therefore, the effect of combining calcium hydroxide and ellagic acid must be researched to reduce cell damage due to the application of calcium hydroxide. The objective of the study was to determine the cytotoxicity and proliferation of fibroblasts after combining calcium hydroxide and ellagic acid with ratios of 99:1, 98:2, 97:3, 96:4, and 95:5. Calcium hydroxide and ellagic acid with different ratios were mixed with water and stirred. Rat gingival fibroblasts were prepared and incubated in two 96-well microplates. The control group and treatment groups (16 samples) were placed in the microplate and incubated for 1 and 3 days. An MTT assay test was performed, and the absorbance was observed using the ELISA reader with a wavelength of 540 nm. Following that, the cell viability was calculated. The results were tabulated and analyzed using a one-way ANOVA. For all treatment groups, the fibroblast cells showed a viability of higher than 50%. There was a significant increase (P < 0.05) in the fibroblast cell proliferation after combining calcium hydroxide and ellagic acid with ratios of 99:1 and 97:3. The combination of calcium hydroxide and ellagic acid is nontoxic. The treatment groups with ratios of 99:1 and 97:3 showed increased fibroblast cell proliferation. Wolters Kluwer - Medknow 2021 2021-01-09 /pmc/articles/PMC7832190/ /pubmed/33532351 http://dx.doi.org/10.4103/japtr.JAPTR_154_20 Text en Copyright: © 2021 Journal of Advanced Pharmaceutical Technology & Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Nirwana, Intan
Munadziroh, Elly
Yogiartono, R. Mohammad
Thiyagu, Candrika
Ying, Chong Sin
Dinaryanti, Aristika
Cytotoxicity and proliferation evaluation on fibroblast after combining calcium hydroxide and ellagic acid
title Cytotoxicity and proliferation evaluation on fibroblast after combining calcium hydroxide and ellagic acid
title_full Cytotoxicity and proliferation evaluation on fibroblast after combining calcium hydroxide and ellagic acid
title_fullStr Cytotoxicity and proliferation evaluation on fibroblast after combining calcium hydroxide and ellagic acid
title_full_unstemmed Cytotoxicity and proliferation evaluation on fibroblast after combining calcium hydroxide and ellagic acid
title_short Cytotoxicity and proliferation evaluation on fibroblast after combining calcium hydroxide and ellagic acid
title_sort cytotoxicity and proliferation evaluation on fibroblast after combining calcium hydroxide and ellagic acid
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7832190/
https://www.ncbi.nlm.nih.gov/pubmed/33532351
http://dx.doi.org/10.4103/japtr.JAPTR_154_20
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