Cargando…

Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses

Lactobacillus helveticus carries many properties such as the ability to survive gastrointestinal transit, modulate the host immune response, accumulate biopeptides in milk, and adhere to the epithelial cells that could contribute to improving host health. In this study, the applicability as function...

Descripción completa

Detalles Bibliográficos
Autores principales: Zago, Miriam, Massimiliano, Lucia, Bonvini, Barbara, Penna, Giuseppe, Giraffa, Giorgio, Rescigno, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833162/
https://www.ncbi.nlm.nih.gov/pubmed/33493208
http://dx.doi.org/10.1371/journal.pone.0245903
_version_ 1783642002080923648
author Zago, Miriam
Massimiliano, Lucia
Bonvini, Barbara
Penna, Giuseppe
Giraffa, Giorgio
Rescigno, Maria
author_facet Zago, Miriam
Massimiliano, Lucia
Bonvini, Barbara
Penna, Giuseppe
Giraffa, Giorgio
Rescigno, Maria
author_sort Zago, Miriam
collection PubMed
description Lactobacillus helveticus carries many properties such as the ability to survive gastrointestinal transit, modulate the host immune response, accumulate biopeptides in milk, and adhere to the epithelial cells that could contribute to improving host health. In this study, the applicability as functional cultures of four L. helveticus strains isolated from Italian hard cheeses was investigated. A preliminary strain characterization showed that the ability to produce folate was generally low while antioxidant, proteolytic, peptidase, and β-galactosidase activities resulted high, although very variable, between strains. When stimulated moDCs were incubated in the presence of live cells, a dose-dependent release of both the pro-inflammatory cytokine IL-12p70 and the anti-inflammatory cytokine IL-10, was shown for all the four strains. In the presence of cell-free culture supernatants (postbiotics), a dose-dependent, decrease of IL-12p70 and an increase of IL-10 was generally observed. The immunomodulatory effect took place also in Caciotta-like cheese made with strains SIM12 and SIS16 as bifunctional (i.e., immunomodulant and acidifying) starter cultures, thus confirming tests in culture media. Given that the growth of bacteria in the cheese was not necessary (they were killed by pasteurization), the results indicated that some constituents of non-viable bacteria had immunomodulatory properties. This study adds additional evidence for the positive role of L. helveticus on human health and suggests cheese as a suitable food for delivering candidate strains and modulating their anti-inflammatory properties.
format Online
Article
Text
id pubmed-7833162
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-78331622021-01-26 Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses Zago, Miriam Massimiliano, Lucia Bonvini, Barbara Penna, Giuseppe Giraffa, Giorgio Rescigno, Maria PLoS One Research Article Lactobacillus helveticus carries many properties such as the ability to survive gastrointestinal transit, modulate the host immune response, accumulate biopeptides in milk, and adhere to the epithelial cells that could contribute to improving host health. In this study, the applicability as functional cultures of four L. helveticus strains isolated from Italian hard cheeses was investigated. A preliminary strain characterization showed that the ability to produce folate was generally low while antioxidant, proteolytic, peptidase, and β-galactosidase activities resulted high, although very variable, between strains. When stimulated moDCs were incubated in the presence of live cells, a dose-dependent release of both the pro-inflammatory cytokine IL-12p70 and the anti-inflammatory cytokine IL-10, was shown for all the four strains. In the presence of cell-free culture supernatants (postbiotics), a dose-dependent, decrease of IL-12p70 and an increase of IL-10 was generally observed. The immunomodulatory effect took place also in Caciotta-like cheese made with strains SIM12 and SIS16 as bifunctional (i.e., immunomodulant and acidifying) starter cultures, thus confirming tests in culture media. Given that the growth of bacteria in the cheese was not necessary (they were killed by pasteurization), the results indicated that some constituents of non-viable bacteria had immunomodulatory properties. This study adds additional evidence for the positive role of L. helveticus on human health and suggests cheese as a suitable food for delivering candidate strains and modulating their anti-inflammatory properties. Public Library of Science 2021-01-25 /pmc/articles/PMC7833162/ /pubmed/33493208 http://dx.doi.org/10.1371/journal.pone.0245903 Text en © 2021 Zago et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zago, Miriam
Massimiliano, Lucia
Bonvini, Barbara
Penna, Giuseppe
Giraffa, Giorgio
Rescigno, Maria
Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses
title Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses
title_full Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses
title_fullStr Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses
title_full_unstemmed Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses
title_short Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses
title_sort functional characterization and immunomodulatory properties of lactobacillus helveticus strains isolated from italian hard cheeses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833162/
https://www.ncbi.nlm.nih.gov/pubmed/33493208
http://dx.doi.org/10.1371/journal.pone.0245903
work_keys_str_mv AT zagomiriam functionalcharacterizationandimmunomodulatorypropertiesoflactobacillushelveticusstrainsisolatedfromitalianhardcheeses
AT massimilianolucia functionalcharacterizationandimmunomodulatorypropertiesoflactobacillushelveticusstrainsisolatedfromitalianhardcheeses
AT bonvinibarbara functionalcharacterizationandimmunomodulatorypropertiesoflactobacillushelveticusstrainsisolatedfromitalianhardcheeses
AT pennagiuseppe functionalcharacterizationandimmunomodulatorypropertiesoflactobacillushelveticusstrainsisolatedfromitalianhardcheeses
AT giraffagiorgio functionalcharacterizationandimmunomodulatorypropertiesoflactobacillushelveticusstrainsisolatedfromitalianhardcheeses
AT rescignomaria functionalcharacterizationandimmunomodulatorypropertiesoflactobacillushelveticusstrainsisolatedfromitalianhardcheeses