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Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses
Lactobacillus helveticus carries many properties such as the ability to survive gastrointestinal transit, modulate the host immune response, accumulate biopeptides in milk, and adhere to the epithelial cells that could contribute to improving host health. In this study, the applicability as function...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833162/ https://www.ncbi.nlm.nih.gov/pubmed/33493208 http://dx.doi.org/10.1371/journal.pone.0245903 |
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author | Zago, Miriam Massimiliano, Lucia Bonvini, Barbara Penna, Giuseppe Giraffa, Giorgio Rescigno, Maria |
author_facet | Zago, Miriam Massimiliano, Lucia Bonvini, Barbara Penna, Giuseppe Giraffa, Giorgio Rescigno, Maria |
author_sort | Zago, Miriam |
collection | PubMed |
description | Lactobacillus helveticus carries many properties such as the ability to survive gastrointestinal transit, modulate the host immune response, accumulate biopeptides in milk, and adhere to the epithelial cells that could contribute to improving host health. In this study, the applicability as functional cultures of four L. helveticus strains isolated from Italian hard cheeses was investigated. A preliminary strain characterization showed that the ability to produce folate was generally low while antioxidant, proteolytic, peptidase, and β-galactosidase activities resulted high, although very variable, between strains. When stimulated moDCs were incubated in the presence of live cells, a dose-dependent release of both the pro-inflammatory cytokine IL-12p70 and the anti-inflammatory cytokine IL-10, was shown for all the four strains. In the presence of cell-free culture supernatants (postbiotics), a dose-dependent, decrease of IL-12p70 and an increase of IL-10 was generally observed. The immunomodulatory effect took place also in Caciotta-like cheese made with strains SIM12 and SIS16 as bifunctional (i.e., immunomodulant and acidifying) starter cultures, thus confirming tests in culture media. Given that the growth of bacteria in the cheese was not necessary (they were killed by pasteurization), the results indicated that some constituents of non-viable bacteria had immunomodulatory properties. This study adds additional evidence for the positive role of L. helveticus on human health and suggests cheese as a suitable food for delivering candidate strains and modulating their anti-inflammatory properties. |
format | Online Article Text |
id | pubmed-7833162 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78331622021-01-26 Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses Zago, Miriam Massimiliano, Lucia Bonvini, Barbara Penna, Giuseppe Giraffa, Giorgio Rescigno, Maria PLoS One Research Article Lactobacillus helveticus carries many properties such as the ability to survive gastrointestinal transit, modulate the host immune response, accumulate biopeptides in milk, and adhere to the epithelial cells that could contribute to improving host health. In this study, the applicability as functional cultures of four L. helveticus strains isolated from Italian hard cheeses was investigated. A preliminary strain characterization showed that the ability to produce folate was generally low while antioxidant, proteolytic, peptidase, and β-galactosidase activities resulted high, although very variable, between strains. When stimulated moDCs were incubated in the presence of live cells, a dose-dependent release of both the pro-inflammatory cytokine IL-12p70 and the anti-inflammatory cytokine IL-10, was shown for all the four strains. In the presence of cell-free culture supernatants (postbiotics), a dose-dependent, decrease of IL-12p70 and an increase of IL-10 was generally observed. The immunomodulatory effect took place also in Caciotta-like cheese made with strains SIM12 and SIS16 as bifunctional (i.e., immunomodulant and acidifying) starter cultures, thus confirming tests in culture media. Given that the growth of bacteria in the cheese was not necessary (they were killed by pasteurization), the results indicated that some constituents of non-viable bacteria had immunomodulatory properties. This study adds additional evidence for the positive role of L. helveticus on human health and suggests cheese as a suitable food for delivering candidate strains and modulating their anti-inflammatory properties. Public Library of Science 2021-01-25 /pmc/articles/PMC7833162/ /pubmed/33493208 http://dx.doi.org/10.1371/journal.pone.0245903 Text en © 2021 Zago et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zago, Miriam Massimiliano, Lucia Bonvini, Barbara Penna, Giuseppe Giraffa, Giorgio Rescigno, Maria Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses |
title | Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses |
title_full | Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses |
title_fullStr | Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses |
title_full_unstemmed | Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses |
title_short | Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses |
title_sort | functional characterization and immunomodulatory properties of lactobacillus helveticus strains isolated from italian hard cheeses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833162/ https://www.ncbi.nlm.nih.gov/pubmed/33493208 http://dx.doi.org/10.1371/journal.pone.0245903 |
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