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Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum
BACKGROUND: It is unclear if amianthoid transformation (AT) of costal cartilage extracellular matrix (ECM) has an impact on the development of pectus excavatum (PE) and pectus carinatum (PC). METHODS: AT foci were examined in intrasurgical biopsy specimens of costal cartilages of children (8–17 year...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833175/ https://www.ncbi.nlm.nih.gov/pubmed/33493174 http://dx.doi.org/10.1371/journal.pone.0245159 |
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author | Kurkov, Alexandr Guller, Anna Fayzullin, Alexey Fayzullinа, Nafisa Plyakin, Vladimir Kotova, Svetlana Timashev, Petr Frolova, Anastasia Kurtak, Nikita Paukov, Vyacheslav Shekhter, Anatoly |
author_facet | Kurkov, Alexandr Guller, Anna Fayzullin, Alexey Fayzullinа, Nafisa Plyakin, Vladimir Kotova, Svetlana Timashev, Petr Frolova, Anastasia Kurtak, Nikita Paukov, Vyacheslav Shekhter, Anatoly |
author_sort | Kurkov, Alexandr |
collection | PubMed |
description | BACKGROUND: It is unclear if amianthoid transformation (AT) of costal cartilage extracellular matrix (ECM) has an impact on the development of pectus excavatum (PE) and pectus carinatum (PC). METHODS: AT foci were examined in intrasurgical biopsy specimens of costal cartilages of children (8–17 years old) with PE (n = 12) and PC (n = 12) and in age-matching autopsy control samples (n = 10) using histological and immunohistochemical staining, atomic force and nonlinear optical microscopy, transmission and scanning electron microscopy, morphometry and statistics. RESULTS: AT areas were identified in the costal cartilage ECM in children with normal chest, PE and PC. Each type of the AT areas (“canonical”, “intertwined”, “fine-fibred” and “intralacunary”) had a unique morphological pattern of thickness and alignment of amianthoid fibers (AFs). AFs were formed via lateral aggregation of collagen type II fibrils in the intact ECM. Foci of the AT were observed significantly more frequently in the PE and PC groups. The AT areas had unique quantitative features in each study group. CONCLUSION: AT is a structurally diverse form of ECM alteration present in healthy and pathological costal cartilage. PE and PC are associated with specific AT disorders. |
format | Online Article Text |
id | pubmed-7833175 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78331752021-01-26 Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum Kurkov, Alexandr Guller, Anna Fayzullin, Alexey Fayzullinа, Nafisa Plyakin, Vladimir Kotova, Svetlana Timashev, Petr Frolova, Anastasia Kurtak, Nikita Paukov, Vyacheslav Shekhter, Anatoly PLoS One Research Article BACKGROUND: It is unclear if amianthoid transformation (AT) of costal cartilage extracellular matrix (ECM) has an impact on the development of pectus excavatum (PE) and pectus carinatum (PC). METHODS: AT foci were examined in intrasurgical biopsy specimens of costal cartilages of children (8–17 years old) with PE (n = 12) and PC (n = 12) and in age-matching autopsy control samples (n = 10) using histological and immunohistochemical staining, atomic force and nonlinear optical microscopy, transmission and scanning electron microscopy, morphometry and statistics. RESULTS: AT areas were identified in the costal cartilage ECM in children with normal chest, PE and PC. Each type of the AT areas (“canonical”, “intertwined”, “fine-fibred” and “intralacunary”) had a unique morphological pattern of thickness and alignment of amianthoid fibers (AFs). AFs were formed via lateral aggregation of collagen type II fibrils in the intact ECM. Foci of the AT were observed significantly more frequently in the PE and PC groups. The AT areas had unique quantitative features in each study group. CONCLUSION: AT is a structurally diverse form of ECM alteration present in healthy and pathological costal cartilage. PE and PC are associated with specific AT disorders. Public Library of Science 2021-01-25 /pmc/articles/PMC7833175/ /pubmed/33493174 http://dx.doi.org/10.1371/journal.pone.0245159 Text en © 2021 Kurkov et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kurkov, Alexandr Guller, Anna Fayzullin, Alexey Fayzullinа, Nafisa Plyakin, Vladimir Kotova, Svetlana Timashev, Petr Frolova, Anastasia Kurtak, Nikita Paukov, Vyacheslav Shekhter, Anatoly Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum |
title | Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum |
title_full | Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum |
title_fullStr | Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum |
title_full_unstemmed | Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum |
title_short | Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum |
title_sort | amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833175/ https://www.ncbi.nlm.nih.gov/pubmed/33493174 http://dx.doi.org/10.1371/journal.pone.0245159 |
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