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Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum

BACKGROUND: It is unclear if amianthoid transformation (AT) of costal cartilage extracellular matrix (ECM) has an impact on the development of pectus excavatum (PE) and pectus carinatum (PC). METHODS: AT foci were examined in intrasurgical biopsy specimens of costal cartilages of children (8–17 year...

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Autores principales: Kurkov, Alexandr, Guller, Anna, Fayzullin, Alexey, Fayzullinа, Nafisa, Plyakin, Vladimir, Kotova, Svetlana, Timashev, Petr, Frolova, Anastasia, Kurtak, Nikita, Paukov, Vyacheslav, Shekhter, Anatoly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833175/
https://www.ncbi.nlm.nih.gov/pubmed/33493174
http://dx.doi.org/10.1371/journal.pone.0245159
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author Kurkov, Alexandr
Guller, Anna
Fayzullin, Alexey
Fayzullinа, Nafisa
Plyakin, Vladimir
Kotova, Svetlana
Timashev, Petr
Frolova, Anastasia
Kurtak, Nikita
Paukov, Vyacheslav
Shekhter, Anatoly
author_facet Kurkov, Alexandr
Guller, Anna
Fayzullin, Alexey
Fayzullinа, Nafisa
Plyakin, Vladimir
Kotova, Svetlana
Timashev, Petr
Frolova, Anastasia
Kurtak, Nikita
Paukov, Vyacheslav
Shekhter, Anatoly
author_sort Kurkov, Alexandr
collection PubMed
description BACKGROUND: It is unclear if amianthoid transformation (AT) of costal cartilage extracellular matrix (ECM) has an impact on the development of pectus excavatum (PE) and pectus carinatum (PC). METHODS: AT foci were examined in intrasurgical biopsy specimens of costal cartilages of children (8–17 years old) with PE (n = 12) and PC (n = 12) and in age-matching autopsy control samples (n = 10) using histological and immunohistochemical staining, atomic force and nonlinear optical microscopy, transmission and scanning electron microscopy, morphometry and statistics. RESULTS: AT areas were identified in the costal cartilage ECM in children with normal chest, PE and PC. Each type of the AT areas (“canonical”, “intertwined”, “fine-fibred” and “intralacunary”) had a unique morphological pattern of thickness and alignment of amianthoid fibers (AFs). AFs were formed via lateral aggregation of collagen type II fibrils in the intact ECM. Foci of the AT were observed significantly more frequently in the PE and PC groups. The AT areas had unique quantitative features in each study group. CONCLUSION: AT is a structurally diverse form of ECM alteration present in healthy and pathological costal cartilage. PE and PC are associated with specific AT disorders.
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spelling pubmed-78331752021-01-26 Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum Kurkov, Alexandr Guller, Anna Fayzullin, Alexey Fayzullinа, Nafisa Plyakin, Vladimir Kotova, Svetlana Timashev, Petr Frolova, Anastasia Kurtak, Nikita Paukov, Vyacheslav Shekhter, Anatoly PLoS One Research Article BACKGROUND: It is unclear if amianthoid transformation (AT) of costal cartilage extracellular matrix (ECM) has an impact on the development of pectus excavatum (PE) and pectus carinatum (PC). METHODS: AT foci were examined in intrasurgical biopsy specimens of costal cartilages of children (8–17 years old) with PE (n = 12) and PC (n = 12) and in age-matching autopsy control samples (n = 10) using histological and immunohistochemical staining, atomic force and nonlinear optical microscopy, transmission and scanning electron microscopy, morphometry and statistics. RESULTS: AT areas were identified in the costal cartilage ECM in children with normal chest, PE and PC. Each type of the AT areas (“canonical”, “intertwined”, “fine-fibred” and “intralacunary”) had a unique morphological pattern of thickness and alignment of amianthoid fibers (AFs). AFs were formed via lateral aggregation of collagen type II fibrils in the intact ECM. Foci of the AT were observed significantly more frequently in the PE and PC groups. The AT areas had unique quantitative features in each study group. CONCLUSION: AT is a structurally diverse form of ECM alteration present in healthy and pathological costal cartilage. PE and PC are associated with specific AT disorders. Public Library of Science 2021-01-25 /pmc/articles/PMC7833175/ /pubmed/33493174 http://dx.doi.org/10.1371/journal.pone.0245159 Text en © 2021 Kurkov et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kurkov, Alexandr
Guller, Anna
Fayzullin, Alexey
Fayzullinа, Nafisa
Plyakin, Vladimir
Kotova, Svetlana
Timashev, Petr
Frolova, Anastasia
Kurtak, Nikita
Paukov, Vyacheslav
Shekhter, Anatoly
Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum
title Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum
title_full Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum
title_fullStr Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum
title_full_unstemmed Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum
title_short Amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum
title_sort amianthoid transformation of costal cartilage matrix in children with pectus excavatum and pectus carinatum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833175/
https://www.ncbi.nlm.nih.gov/pubmed/33493174
http://dx.doi.org/10.1371/journal.pone.0245159
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