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Study on the region-specific expression of epididymis mRNA in the rams
The epididymis is divided into three regions including the caput, corpus and cauda. Gene expression profiles in different regions indicate the different functions of epididymis which are crucial for sperm maturation. In this study, three one-year-old rams was used as the experimental animal. Transcr...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833257/ https://www.ncbi.nlm.nih.gov/pubmed/33493206 http://dx.doi.org/10.1371/journal.pone.0245933 |
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author | Wu, Cuiling Wang, Chunxin Zhai, Bo Zhao, Yunhui Zhao, Zhuo Yuan, Zhiyu Fu, Xuefeng Zhang, Mingxin |
author_facet | Wu, Cuiling Wang, Chunxin Zhai, Bo Zhao, Yunhui Zhao, Zhuo Yuan, Zhiyu Fu, Xuefeng Zhang, Mingxin |
author_sort | Wu, Cuiling |
collection | PubMed |
description | The epididymis is divided into three regions including the caput, corpus and cauda. Gene expression profiles in different regions indicate the different functions of epididymis which are crucial for sperm maturation. In this study, three one-year-old rams was used as the experimental animal. Transcriptome sequencing technology was used to sequence mRNA in the caput, corpus and cauda of the epididymis. Based on the spatiotemporal-specific expression pattern in the epididymis, the mRNA expression profiles of the three parts of the epididymis were analysed. Region-specifically expressed genes were analysed by GO and KEGG analyses to screen the key genes involved in sheep sperm maturation. We obtained 129, 54 and 99 specifically expressed genes in the caput, corpus and cauda, respectively. And twenty specific expressed genes related to sperm maturation were used to construct functional networks. The heatmap showed that 6 genes of LCN protein family were highly expressed in the head of epididymis of sheep. We infer that sperm maturation is gradual in the epididymis and that there are significant differences in epididymal gene expression patterns between different species. This provides a data resource for analysing the regulatory mechanism of epididymis genes related to sperm maturation in rams. |
format | Online Article Text |
id | pubmed-7833257 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78332572021-01-26 Study on the region-specific expression of epididymis mRNA in the rams Wu, Cuiling Wang, Chunxin Zhai, Bo Zhao, Yunhui Zhao, Zhuo Yuan, Zhiyu Fu, Xuefeng Zhang, Mingxin PLoS One Research Article The epididymis is divided into three regions including the caput, corpus and cauda. Gene expression profiles in different regions indicate the different functions of epididymis which are crucial for sperm maturation. In this study, three one-year-old rams was used as the experimental animal. Transcriptome sequencing technology was used to sequence mRNA in the caput, corpus and cauda of the epididymis. Based on the spatiotemporal-specific expression pattern in the epididymis, the mRNA expression profiles of the three parts of the epididymis were analysed. Region-specifically expressed genes were analysed by GO and KEGG analyses to screen the key genes involved in sheep sperm maturation. We obtained 129, 54 and 99 specifically expressed genes in the caput, corpus and cauda, respectively. And twenty specific expressed genes related to sperm maturation were used to construct functional networks. The heatmap showed that 6 genes of LCN protein family were highly expressed in the head of epididymis of sheep. We infer that sperm maturation is gradual in the epididymis and that there are significant differences in epididymal gene expression patterns between different species. This provides a data resource for analysing the regulatory mechanism of epididymis genes related to sperm maturation in rams. Public Library of Science 2021-01-25 /pmc/articles/PMC7833257/ /pubmed/33493206 http://dx.doi.org/10.1371/journal.pone.0245933 Text en © 2021 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wu, Cuiling Wang, Chunxin Zhai, Bo Zhao, Yunhui Zhao, Zhuo Yuan, Zhiyu Fu, Xuefeng Zhang, Mingxin Study on the region-specific expression of epididymis mRNA in the rams |
title | Study on the region-specific expression of epididymis mRNA in the rams |
title_full | Study on the region-specific expression of epididymis mRNA in the rams |
title_fullStr | Study on the region-specific expression of epididymis mRNA in the rams |
title_full_unstemmed | Study on the region-specific expression of epididymis mRNA in the rams |
title_short | Study on the region-specific expression of epididymis mRNA in the rams |
title_sort | study on the region-specific expression of epididymis mrna in the rams |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833257/ https://www.ncbi.nlm.nih.gov/pubmed/33493206 http://dx.doi.org/10.1371/journal.pone.0245933 |
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