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Growth and Spatial Control of Murine Neural Stem Cells on Reflectin Films
[Image: see text] Stem cells have attracted significant attention due to their regenerative capabilities and their potential for the treatment of disease. Consequently, significant research effort has focused on the development of protein- and polypeptide-based materials as stem cell substrates and...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833438/ https://www.ncbi.nlm.nih.gov/pubmed/33455403 http://dx.doi.org/10.1021/acsbiomaterials.9b00824 |
_version_ | 1783642066029379584 |
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author | Kautz, Rylan Phan, Long Arulmoli, Janahan Chatterjee, Atrouli Kerr, Justin P. Naeim, Mahan Long, James Allevato, Alex Leal-Cruz, Jessica E. Le, LeAnn Derakhshan, Parsa Tombola, Francesco Flanagan, Lisa A. Gorodetsky, Alon A. |
author_facet | Kautz, Rylan Phan, Long Arulmoli, Janahan Chatterjee, Atrouli Kerr, Justin P. Naeim, Mahan Long, James Allevato, Alex Leal-Cruz, Jessica E. Le, LeAnn Derakhshan, Parsa Tombola, Francesco Flanagan, Lisa A. Gorodetsky, Alon A. |
author_sort | Kautz, Rylan |
collection | PubMed |
description | [Image: see text] Stem cells have attracted significant attention due to their regenerative capabilities and their potential for the treatment of disease. Consequently, significant research effort has focused on the development of protein- and polypeptide-based materials as stem cell substrates and scaffolds. Here, we explore the ability of reflectin, a cephalopod structural protein, to support the growth of murine neural stem/progenitor cells (mNSPCs). We observe that the binding, growth, and differentiation of mNSPCs on reflectin films is comparable to that on more established protein-based materials. Moreover, we find that heparin selectively inhibits the adhesion of mNSPCs on reflectin, affording spatial control of cell growth and leading to a >30-fold change in cell density on patterned substrates. The described findings highlight the potential utility of reflectin as a stem cell culture material. |
format | Online Article Text |
id | pubmed-7833438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American
Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-78334382021-01-26 Growth and Spatial Control of Murine Neural Stem Cells on Reflectin Films Kautz, Rylan Phan, Long Arulmoli, Janahan Chatterjee, Atrouli Kerr, Justin P. Naeim, Mahan Long, James Allevato, Alex Leal-Cruz, Jessica E. Le, LeAnn Derakhshan, Parsa Tombola, Francesco Flanagan, Lisa A. Gorodetsky, Alon A. ACS Biomater Sci Eng [Image: see text] Stem cells have attracted significant attention due to their regenerative capabilities and their potential for the treatment of disease. Consequently, significant research effort has focused on the development of protein- and polypeptide-based materials as stem cell substrates and scaffolds. Here, we explore the ability of reflectin, a cephalopod structural protein, to support the growth of murine neural stem/progenitor cells (mNSPCs). We observe that the binding, growth, and differentiation of mNSPCs on reflectin films is comparable to that on more established protein-based materials. Moreover, we find that heparin selectively inhibits the adhesion of mNSPCs on reflectin, affording spatial control of cell growth and leading to a >30-fold change in cell density on patterned substrates. The described findings highlight the potential utility of reflectin as a stem cell culture material. American Chemical Society 2020-01-22 2020-03-09 /pmc/articles/PMC7833438/ /pubmed/33455403 http://dx.doi.org/10.1021/acsbiomaterials.9b00824 Text en This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Kautz, Rylan Phan, Long Arulmoli, Janahan Chatterjee, Atrouli Kerr, Justin P. Naeim, Mahan Long, James Allevato, Alex Leal-Cruz, Jessica E. Le, LeAnn Derakhshan, Parsa Tombola, Francesco Flanagan, Lisa A. Gorodetsky, Alon A. Growth and Spatial Control of Murine Neural Stem Cells on Reflectin Films |
title | Growth and Spatial Control of Murine Neural Stem Cells
on Reflectin Films |
title_full | Growth and Spatial Control of Murine Neural Stem Cells
on Reflectin Films |
title_fullStr | Growth and Spatial Control of Murine Neural Stem Cells
on Reflectin Films |
title_full_unstemmed | Growth and Spatial Control of Murine Neural Stem Cells
on Reflectin Films |
title_short | Growth and Spatial Control of Murine Neural Stem Cells
on Reflectin Films |
title_sort | growth and spatial control of murine neural stem cells
on reflectin films |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833438/ https://www.ncbi.nlm.nih.gov/pubmed/33455403 http://dx.doi.org/10.1021/acsbiomaterials.9b00824 |
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