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An in-well direct lysis method for rapid detection of SARS-CoV-2 by real time RT-PCR in eSwab specimens

BACKGROUND: Diagnostic real time reverse transcription PCR (rRT-PCR) is usually done using nucleic acid (NA) purified from the sample. In the SARS-CoV-2 pandemic reagents and utensils for NA purification has been in short supply. This has generated interest in methods that eliminate the need for NA...

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Detalles Bibliográficos
Autores principales: Jørgensen, Rikke Lind, Pedersen, Martin Schou, Chauhan, Alisha Shazad, Andreasson, Louise Munkholm, Kristiansen, Gitte Qvist, Lisby, Jan Gorm, Rosenstierne, Maiken Worsøe, Schønning, Kristian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833634/
https://www.ncbi.nlm.nih.gov/pubmed/33428990
http://dx.doi.org/10.1016/j.jviromet.2021.114062