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Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors
The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has devastated the global economy and claimed more than 1.7 million lives, presenting an urgent global health crisis. To identify host factors required for infection by SARS-CoV-2 and seasonal coronaviruses, we designe...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833927/ https://www.ncbi.nlm.nih.gov/pubmed/33357464 http://dx.doi.org/10.1016/j.chom.2020.12.009 |
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author | Hoffmann, H.-Heinrich Sánchez-Rivera, Francisco J. Schneider, William M. Luna, Joseph M. Soto-Feliciano, Yadira M. Ashbrook, Alison W. Le Pen, Jérémie Leal, Andrew A. Ricardo-Lax, Inna Michailidis, Eleftherios Hao, Yuan Stenzel, Ansgar F. Peace, Avery Zuber, Johannes Allis, C. David Lowe, Scott W. MacDonald, Margaret R. Poirier, John T. Rice, Charles M. |
author_facet | Hoffmann, H.-Heinrich Sánchez-Rivera, Francisco J. Schneider, William M. Luna, Joseph M. Soto-Feliciano, Yadira M. Ashbrook, Alison W. Le Pen, Jérémie Leal, Andrew A. Ricardo-Lax, Inna Michailidis, Eleftherios Hao, Yuan Stenzel, Ansgar F. Peace, Avery Zuber, Johannes Allis, C. David Lowe, Scott W. MacDonald, Margaret R. Poirier, John T. Rice, Charles M. |
author_sort | Hoffmann, H.-Heinrich |
collection | PubMed |
description | The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has devastated the global economy and claimed more than 1.7 million lives, presenting an urgent global health crisis. To identify host factors required for infection by SARS-CoV-2 and seasonal coronaviruses, we designed a focused high-coverage CRISPR-Cas9 library targeting 332 members of a recently published SARS-CoV-2 protein interactome. We leveraged the compact nature of this library to systematically screen SARS-CoV-2 at two physiologically relevant temperatures along with three related coronaviruses (human coronavirus 229E [HCoV-229E], HCoV-NL63, and HCoV-OC43), allowing us to probe this interactome at a much higher resolution than genome-scale studies. This approach yielded several insights, including potential virus-specific differences in Rab GTPase requirements and glycosylphosphatidylinositol (GPI) anchor biosynthesis, as well as identification of multiple pan-coronavirus factors involved in cholesterol homeostasis. This coronavirus essentiality catalog could inform ongoing drug development efforts aimed at intercepting and treating coronavirus disease 2019 (COVID-19) and help prepare for future coronavirus outbreaks. |
format | Online Article Text |
id | pubmed-7833927 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78339272021-01-26 Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors Hoffmann, H.-Heinrich Sánchez-Rivera, Francisco J. Schneider, William M. Luna, Joseph M. Soto-Feliciano, Yadira M. Ashbrook, Alison W. Le Pen, Jérémie Leal, Andrew A. Ricardo-Lax, Inna Michailidis, Eleftherios Hao, Yuan Stenzel, Ansgar F. Peace, Avery Zuber, Johannes Allis, C. David Lowe, Scott W. MacDonald, Margaret R. Poirier, John T. Rice, Charles M. Cell Host Microbe Resource The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has devastated the global economy and claimed more than 1.7 million lives, presenting an urgent global health crisis. To identify host factors required for infection by SARS-CoV-2 and seasonal coronaviruses, we designed a focused high-coverage CRISPR-Cas9 library targeting 332 members of a recently published SARS-CoV-2 protein interactome. We leveraged the compact nature of this library to systematically screen SARS-CoV-2 at two physiologically relevant temperatures along with three related coronaviruses (human coronavirus 229E [HCoV-229E], HCoV-NL63, and HCoV-OC43), allowing us to probe this interactome at a much higher resolution than genome-scale studies. This approach yielded several insights, including potential virus-specific differences in Rab GTPase requirements and glycosylphosphatidylinositol (GPI) anchor biosynthesis, as well as identification of multiple pan-coronavirus factors involved in cholesterol homeostasis. This coronavirus essentiality catalog could inform ongoing drug development efforts aimed at intercepting and treating coronavirus disease 2019 (COVID-19) and help prepare for future coronavirus outbreaks. Elsevier Inc. 2021-02-10 2020-12-16 /pmc/articles/PMC7833927/ /pubmed/33357464 http://dx.doi.org/10.1016/j.chom.2020.12.009 Text en © 2020 Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Resource Hoffmann, H.-Heinrich Sánchez-Rivera, Francisco J. Schneider, William M. Luna, Joseph M. Soto-Feliciano, Yadira M. Ashbrook, Alison W. Le Pen, Jérémie Leal, Andrew A. Ricardo-Lax, Inna Michailidis, Eleftherios Hao, Yuan Stenzel, Ansgar F. Peace, Avery Zuber, Johannes Allis, C. David Lowe, Scott W. MacDonald, Margaret R. Poirier, John T. Rice, Charles M. Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors |
title | Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors |
title_full | Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors |
title_fullStr | Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors |
title_full_unstemmed | Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors |
title_short | Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors |
title_sort | functional interrogation of a sars-cov-2 host protein interactome identifies unique and shared coronavirus host factors |
topic | Resource |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833927/ https://www.ncbi.nlm.nih.gov/pubmed/33357464 http://dx.doi.org/10.1016/j.chom.2020.12.009 |
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