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Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori

The canonical chemotaxis network modulates the bias for a particular direction of rotation in the bacterial flagellar motor to help the cell migrate toward favorable chemical environments. How the chemotaxis network in Helicobacter pylori modulates flagellar functions is unknown, which limits our un...

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Detalles Bibliográficos
Autores principales: Antani, Jyot D, Sumali, Anita X, Lele, Tanmay P, Lele, Pushkar P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834020/
https://www.ncbi.nlm.nih.gov/pubmed/33493107
http://dx.doi.org/10.7554/eLife.63936
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author Antani, Jyot D
Sumali, Anita X
Lele, Tanmay P
Lele, Pushkar P
author_facet Antani, Jyot D
Sumali, Anita X
Lele, Tanmay P
Lele, Pushkar P
author_sort Antani, Jyot D
collection PubMed
description The canonical chemotaxis network modulates the bias for a particular direction of rotation in the bacterial flagellar motor to help the cell migrate toward favorable chemical environments. How the chemotaxis network in Helicobacter pylori modulates flagellar functions is unknown, which limits our understanding of chemotaxis in this species. Here, we determined that H. pylori swim faster (slower) whenever their flagella rotate counterclockwise (clockwise) by analyzing their hydrodynamic interactions with bounding surfaces. This asymmetry in swimming helped quantify the rotational bias. Upon exposure to a chemo-attractant, the bias decreased and the cells tended to swim exclusively in the faster mode. In the absence of a key chemotaxis protein, CheY, the bias was zero. The relationship between the reversal frequency and the rotational bias was unimodal. Thus, H. pylori’s chemotaxis network appears to modulate the probability of clockwise rotation in otherwise counterclockwise-rotating flagella, similar to the canonical network.
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spelling pubmed-78340202021-01-27 Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori Antani, Jyot D Sumali, Anita X Lele, Tanmay P Lele, Pushkar P eLife Physics of Living Systems The canonical chemotaxis network modulates the bias for a particular direction of rotation in the bacterial flagellar motor to help the cell migrate toward favorable chemical environments. How the chemotaxis network in Helicobacter pylori modulates flagellar functions is unknown, which limits our understanding of chemotaxis in this species. Here, we determined that H. pylori swim faster (slower) whenever their flagella rotate counterclockwise (clockwise) by analyzing their hydrodynamic interactions with bounding surfaces. This asymmetry in swimming helped quantify the rotational bias. Upon exposure to a chemo-attractant, the bias decreased and the cells tended to swim exclusively in the faster mode. In the absence of a key chemotaxis protein, CheY, the bias was zero. The relationship between the reversal frequency and the rotational bias was unimodal. Thus, H. pylori’s chemotaxis network appears to modulate the probability of clockwise rotation in otherwise counterclockwise-rotating flagella, similar to the canonical network. eLife Sciences Publications, Ltd 2021-01-25 /pmc/articles/PMC7834020/ /pubmed/33493107 http://dx.doi.org/10.7554/eLife.63936 Text en © 2021, Antani et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Physics of Living Systems
Antani, Jyot D
Sumali, Anita X
Lele, Tanmay P
Lele, Pushkar P
Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori
title Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori
title_full Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori
title_fullStr Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori
title_full_unstemmed Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori
title_short Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in Helicobacter pylori
title_sort asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias in helicobacter pylori
topic Physics of Living Systems
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834020/
https://www.ncbi.nlm.nih.gov/pubmed/33493107
http://dx.doi.org/10.7554/eLife.63936
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